陈敏杰;钱懿宏;于青燕;郭倩;洪智程;徐冬梅;

• 1:浙江树人大学生物与环境工程学院

摘要(Abstract)：

四环素类抗生素(TCs)在畜禽养殖中的大量使用甚至滥用,导致其在动物粪便中高浓度残留。随着畜禽粪便有机肥的农田施用,TCs持续进入土壤并且不断累积,由此带来的土壤生态危害和健康风险值得关注。以四环素(TC)、土霉素(OTC)为研究对象,采用室内培养试验法,考察2种典型TCs对土壤微生物、酶活性的影响及对植物生长的毒性作用。结果表明,低浓度TC和OTC作用下,土壤细菌和真菌数量即显著降低,土壤细菌较真菌对TCs的污染更为敏感。除TC对土壤酸性磷酸酶和OTC对土壤过氧化氢酶活性主要表现为激活作用外,总体上TC、OTC作用后土壤酶活性呈低浓度促进、高浓度抑制的变化趋势。80 mg·L~(-1)的TC、OTC暴露下,绿豆芽芽伸长被显著抑制,并且随着抗生素浓度的增大,绿豆芽伸长抑制率大幅升高。相同浓度、相同暴露时间条件下的TC对绿豆芽伸长的毒性大于OTC。

关键词(KeyWords)： 四环素类抗生素;微生物;土壤;植物生长

Abstract:

Keywords:

基金项目(Foundation): 浙江省自然科学基金项目(LY18B070005);; 国家级大学生创新创业训练计划项目(201811842020);; 浙江树人大学中青年学术团队项目

作者(Author): 陈敏杰;钱懿宏;于青燕;郭倩;洪智程;徐冬梅;

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DOI:

参考文献(References)：

• [1]朱永官,欧阳纬莹,吴楠,等.抗生素耐药性的来源与控制对策[J].中国科学院院刊, 2015, 30(4):509-516Zhu Y G, Ouyang W Y, Wu N, et al. Antibiotic resistance:Sources and mitigation[J]. Bulletin of Chinese Academy of Sciences, 2015, 30(4):509-516(in Chinese)
• [2]Tasho R P, Cho J Y. Veterinary antibiotics in animal waste, its distribution in soil and uptake by plants:A review[J]. Science of the Total Environment, 2016, 563-564:366-376
• [3]Zhang Q Q, Ying G G, Pan C G, et al. Comprehensive evaluation of antibiotics emission and fate in the river basins of China:Source analysis, multimedia modeling, and linkage to bacterial resistance[J]. Environmental Science and Technology, 2015, 49(11):6772-6782
• [4]Yu B H, Zakaria M P, Latif P A, et al. Occurrence of veterinary antibiotics and progesterone in broiler manure and agricultural soil in Ma Iaysia[J]. Science of the Total Environment, 2014, 488-489:261-267
• [5]赵方凯,杨磊,李守娟,等.长三角典型城郊土壤抗生素空间分布的影响因素研究[J].环境科学学报, 2018,38(3):1163-1171Zhao F K, Yang L, Li S J, et al. Affecting factors of the spatial distribution of antibiotics in soils in typical peri-urban area of Yangtze River Delta[J]. Acta Scientiae Circumstantiae, 2018, 38(3):1163-1171(in Chinese)
• [6]Chee-Sanford J C, Mackie R I, Koike S, et al. Fate and transport of antibiotic residues and antibiotic resistance genes following land application of manure waste[J].Journal of Environmental Quality, 2009, 38(3):1086-1108
• [7]Wei R, Ge F, Zhang L, et al. Occurrence of 13 veterinary drugs in animal manure-amended soils in Eastern China[J]. Chemosphere, 2016, 144:2377-2383
• [8]Li Y W, Wu X L, Mo C H, et al. Investigation of sulfonamide, tetracycline, and quinolone antibiotics in vegetable farmland soil in the Pearl River Delta Area, Southern China[J]. Journal of Agricultural and Food Chemistry, 2011,59(13):7268-7276
• [9]阮琳琳,林辉,马军伟,等.土壤中单一及复合抗生素的降解及微生物响应[J].中国环境科学, 2018, 38(3):1081-1089Ruan L L, Lin H, Ma J W, et al. Behavior and ecotoxicity of single and mixed antibiotics in soils:Degradation and changes in microbial community[J]. China Environmental Science, 2018, 38(3):1081-1089(in Chinese)
• [10]Riaz L, Mahmood T, Coyne M S, et al. Physiological and antioxidant response of wheat(Triticum aestivum)seedlings to fluoroquinolone antibiotics[J]. Chemosphere,2017, 177:250-257
• [11]徐一兰,唐海明,肖小平,等.长期施肥对双季稻田土壤微生物学特性的影响[J].生态学报, 2016, 36(18):5847-5855Xu Y L, Tang H M, Xiao X P, et al. Effects of different long-term fertilization regimes on the soil microbiological properties of apaddy field[J]. Acta Ecologica Sinica,2016, 36(18):5847-5855(in Chinese)
• [12]关松荫.土壤酶及其研究法[M].北京:中国农业出版社, 1986:260-360
• [13]赵天瑶,张亚宏,金涛,等.绿豆萌发过程中蛋白组分及亚基变化[J].中国农业科学, 2018, 51(9):1783-1794Zhao T Y, Zhang Y H, Jin T, et al. The changes of protein components and subunits in process of mung bean germination[J]. Scientia Agricultura Sinica, 2018, 51(9):1783-1794(in Chinese)
• [14]罗蓉,杨苗,余旋,等.沙棘人工林土壤微生物群落结构及酶活性的季节变化[J].应用生态学报, 2018, 29(4):1163-1169Luo R, Yang M, Yu X, et al. Seasonal dynamics of soil microbial community and enzyme activities in Hippophae rhamnoides plantation[J]. Chinese Journal of Applied Ecology, 2018, 29(4):1163-1169(in Chinese)
• [15]Rodriguez-Rojas A, Rodriguez-Beltran J, Couce A, et al.Antibiotics and antibiotic resistance:A bitter fight against evolution[J]. International Journal of Medical Microbiology, 2013, 303(6-7):293-297
• [16]王佳宁,徐永平,李晓宇,等.畜禽粪便抗生素残留及其对环境的影响[J].畜牧与兽医, 2017, 49(10):140-144Wang J N, Xu Y P, Li X Y, et al. Environmental impact of livestock and poultry manures with antibiotic residues[J].Animal Husbandry&Veterinary Medicine, 2017, 49(10):140-144(in Chinese)
• [17]张慧敏,章明奎,顾国平.浙北地区畜禽粪便和农田土壤中四环素类抗生素残留[J].生态与农村环境学报,2008, 24(3):69-73Zhang H M, Zhang M K, Gu G P. Residues of tetracyclines in livestock and poultry manures and agricultural soils from North Zhejiang Province[J]. Journal of Ecology&Rural Environment, 2008, 24(3):69-73(in Chinese)
• [18]Hamscher G, Sczesny S, H?per H, et al. Determination of persistent tetracycline residues in soil fertilized with liquid manure by high-performance liquid chromatography with electrospray ionization tandem mass spectrometry[J]. Analytical Chemistry, 2002, 74(7):1509-1518
• [19]成玉婷,吴小莲,向垒,等.广州市典型有机蔬菜基地土壤中磺胺类抗生素污染特征及风险评价[J].中国环境科学, 2017, 37(3):1154-1161Cheng Y T, Wu Y L, Xiang L, et al. Distribution and risk assessment of sulfonamide antibiotics in soil from organic vegetable farms in Guangzhou[J]. China Environmental Science, 2017, 37(3):1154-1161(in Chinese)
• [20]Yang Q X, Zhang J, Zhu K F, et al. Influence of oxytetracycline on the structure and activity of microbial community in wheat rhizosphere soil[J]. Journal of Environmental Sciences, 2009, 21(7):954-959
• [21]徐晨光,张奇春,侯昌萍.外源抗生素对茶园土壤微生物群落结构的影响[J].浙江大学学报:农业与生命科学版, 2014, 40(1):75-84Xu C G, Zhang Q C, Hou C P. Effect of exogenous antibiotics on soil microbial community structure in tea garden[J]. Journal of Zhejian University:Agriculture&Life Sciences, 2014, 40(1):75-84(in Chinese)
• [22]张浩,罗义,周启星.四环素类抗生素生态毒性研究进展[J].农业环境科学学报, 2008, 27(2):407-413Zhang H, Luo Y, Zhou Q X. Research advancement of eco-toxicity of tetracycline antibiotics[J]. Journal of Agro-Environment Science, 2008, 27(2):407-413(in Chinese)
• [23]杨青华,韩锦峰.棉田不同覆盖方式对土壤微生物和酶活性的影响[J].土壤学报, 2005, 42(2):348-351Yang Q H, Han J F. Effects of mulching on soil microorganisms and enzyme activities in cotton field[J]. Acta Pedologica Sinica, 2005, 42(2):348-351(in Chinese)
• [24]Kong W D, Zhu Y G, Liang Y C, et al. Uptake of oxytetracycline and its phytotoxicity to alfalfa[J]. Environmental Pollution, 2007, 147(1):187-193
• [25]刘娣,卢玲丽,罗伟君,等.土壤中四环素类抗生素降解及对小白菜根系生长发育的影响[J].环境科学学报,2017, 37(5):1957-1966Liu D, Lu L L, Luo W J, et al. Degradation of tetracyclines in soils and their effects on root growth of Chinese cabbages(Brassica campestris L.)[J]. Acta Scientiae Circumstantiae, 2017, 37(5):1957-1966(in Chinese)
• [26]Farkas M H, Berry J O, Aga D S. Chlortetracycline detoxification in maize via induction of glutathione S-transferases after antibiotic exposure[J]. Environmental Science&Technology, 2007, 41(4):1450-1456