张春雷;李洪志;刘洁婷;包海花;吴丹;初彦辉;赵冰海;

• 1:黑龙江省抗纤维化生物治疗重点实验室 牡丹江医学院医药研究中心

摘要(Abstract)：

为研究全氟辛烷磺酸盐(PFOS)暴露对胎鼠肺部损伤的诱导作用,孕期SD大鼠在5～20mg·kg-1剂量范围内的PFOS中处理7d,取胎鼠全肺并分析其发育所受的影响。通过形态学比较,发现随着PFOS浓度的增加,胎鼠体长和体重均显著降低,高剂量暴露会导致胎鼠死亡。通过组织学检测,发现胎鼠肺的发育受到PFOS暴露的抑制。通过WesternBlot检测肺泡Ⅰ/Ⅱ型细胞的发育,发现肺泡Ⅰ型细胞特异蛋白Podoplanin表达显著减少(p<0.05),肺泡Ⅱ型细胞特异蛋白SP-C表达减少但未出现显著差异,此外,与对照组相比高剂量暴露会引起血管内皮生长因子(VEGF)表达显著减少(p<0.01)。实验结果说明,PFOS暴露会导致胎鼠肺部发育出现损伤,这种损伤可能是肺泡Ⅰ型细胞及肺部血管发育受抑制引起胎鼠肺部气体交换功能破坏。

关键词(KeyWords)： 全氟辛烷磺酸盐;胎鼠;肺泡细胞;血管内皮生长因子;发育毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81102149-H2607);; 黑龙江省卫生厅科研课题项目(2010-235,2010-238);; 牡丹江医学院科学技术研究项目(2011-16)

作者(Author): 张春雷;李洪志;刘洁婷;包海花;吴丹;初彦辉;赵冰海;

Email:

DOI:

参考文献(References)：

• [1]Lau C,Thibodeaux J R,Hanson R G,et al.Exposure to perfluorooctane sulfonate during pregnancy in rat and mouse.II:Postnatal evaluation[J].Toxicological Sci-ences,2003,74(2):382-392
• [2]Giesy J P,Kannan K.Global distribution of perfluo-rooctane sulfonate in wildlife[J].Environmental Science&Technology,2001,35(7):1339-1342
• [3]Pelley J.Canada moves to eliminate PFOS stain repel-lents[J].Environmental Science&Technology,2004,38(23):452A
• [4]Chen T,Zhang L,Yue J Q,et al.Prenatal PFOS expo-sure induces oxidative stress and apoptosis in the lung of rat off-spring[J].Reproductive Toxicology,2012,33(4):538-545
• [5]Lau C,Anitole K,Hodes C,et al.Perfluoroalkyl acids:A review of monitoring and toxicological findings[J].Toxicological Sciences,2007,99(2):366-394
• [6]Naile J E,Khim J S,Wang T,et al.Perfluorinated compounds in water,sediment,soil and biota from estu-arine and coastal areas of Korea[J].Environmental Pollution,2010,158(5):1237-1244
• [7]Inoue K,Okada F,Ito R,et al.Perfluorooctane sulfon-ate(PFOS)and related perfluorinated compounds in hu-man maternal and cord blood samples:Assessment of PFOS exposure in a susceptible population during preg-nancy[J].Environmental Health Perspectives,2004,112(11):1204-1207
• [8]Dai J,Li M,Jin Y,et al.Perfluorooctanesulfonate and periluorooctanoate in red panda and giant panda from China[J].Environmental Science&Technology,2006,40(18):5647-5652
• [9]Tao L,Kannan K,Kajiwara N,et al.Perfluorooctane-sulfonate and related fluorochemicals in albatrosses,ele-phant seals,penguins,and polar skuas from the South-ern Ocean[J].Environmental Science&Technology,2006,40(24):7642-7648
• [10]Olivero-Verbel J,Tao L,Johnson-Restrepo B,et al.Perfluorooctanesulfonate and related fluorochemicals in biological samples from the north coast of Colombia[J].Environmental Pollution,2006,142(2):367-372
• [11]Kovarova J,Svobodova Z.Perfluorinated compounds:Occurrence and risk profile[J].Neuro Endocrinology Letters,2008,29(5):599-608
• [12]余明东,袁萍,何杰颖,等.全氟辛烷磺酸对SD大鼠的免疫损伤作用[J].中南医学科学杂志.2011,39(2):220-223Yu M,Yuan P,He J,et al.The immunologic injury ef-fection of PFOS on SD rats[J].Journal of Medical Sci-ence in Central South China,2011,39(2):220-223(in Chinese)
• [13]文红玲,李梓民,周艳,等.全氟辛烷磺酸(PFOS)对小鼠免疫功能的影响[J].南华大学学报(医学版),2010,38(1):45-47Wen H,Li Z,Zhou Y,et al.Effect of perfluorooctane sulfonate(PFOS)on immunity in mice[J].Journal of University of South China:Medical Edition,2010,38(1):45-47(in Chinese)
• [14]叶露,吴玲玲,蒋雨希,等.PFOS/PFOA对斑马鱼(Da-nio rerio)胚胎致毒效应研究[J].环境科学,2009,30(6):1727-1732Ye L,Wu L,Jiang Y,et al.Toxicological Study of PFOS/PFOA to zebrafish(Danio rerio)embryos[J].Environmental Science,2009,30(6):1727-1732(in Chinese)
• [15]Mulkiewicz E,Jastorff B,Skladanowski A C,et al.E-valuation of the acute toxicity of perfluorinated car-boxylic acids using eukaryotic cell lines,bacteria and enzymatic assays[J].Environmental Toxicology and Pharmacology,2007,23(3):279-285
• [16]郑丽,董光辉,于一凡,等.全氟辛烷磺酸对小鼠脾细胞NO分泌水平影响[J].中国公共卫生,2010,26(4):431-432Zheng L,Dong G,Yu Y,et al.Effect of perfluo-rooctane sulfonate(PFOS)on nitric oxide secretion in C57BL/6mice[J].Chinese Journal of Public Health,2010,26(4):431-432(in Chinese)
• [17]张小梅,宋锦兰,金一和,等.全氟辛烷磺酸对雄性鹌鹑生殖毒性影响[J].生态毒理学报,2011,6(2):143-148Zhang X,Song J,Jin Y,et al.Study on reproductive toxicity of perfiuorooctane sulfonate(PFOS)in male quail[J].Asian Journal of Ecotoxicology,2011,6(2):143-148(in Chinese)
• [18]Lau C,Thibodeaux J R,Hanson R G,et al.Effects of perfluorooctanoic acid exposure during pregnancy in the mouse[J].Toxicological Sciences.2006,90(2):510-518
• [19]仇敬运,屈艾,胡文静,等.全氟辛烷磺酸钾对小鼠肝脏的氧化性损伤研究[J].毒理学杂志.2011,25(4):265-268Qiu J,Qu A,Hu W,et al.Study on oxidative damage on liver of mice induced by PFOS-K[J].Journal of Toxi-cology.2011,25(4):265-268(in Chinese)
• [20]Han X,Kemper R A,Jepson G W.Subcellular distribu-tion and protein binding of perfluorooctanoic acid in rat liver and kidney[J].Drug and Chemical Toxicology,2005,28(2):197-209
• [21]So M K,Yamashita N,Taniyasu S,et al.Health risks in infants associated with exposure to perfluorinated com-pounds in human breast milk from Zhoushan,China[J].Environmental Science&Technology,2006,40(9):2924-2929
• [22]Olsen G W,Burris J M,Burlew M M,et al.Epidemio-logic assessment of worker serum perfluorooctanesul-fonate(PFOS)and perfluorooctanoate(PFOA)concen-trations and medical surveillance examinations[J].Journal of Occupational and Environmental Medicine,2003,45(3):260-270
• [23]Han X,Snow T A,Kemper R A,et al.Binding of per-fluorooctanoic acid to rat and human plasma proteins[J].Chemical Research in Toxicology.2003,16(6):775-781
• [24]Cui L,Zhou Q F,Liao C Y,et al.Studies on the toxi-cological effects of PFOA and PFOS on rats using his-tological observation and chemical analysis[J].Ar-chives of Environment Contamination and Toxicolo-gy,2009,56(2):338-349