张春雷;李洪志;刘洁婷;包海花;吴丹;初彦辉;赵冰海;

• 1:黑龙江省抗纤维化生物治疗重点实验室 牡丹江医学院医药研究中心

摘要(Abstract)：

为研究全氟辛烷磺酸盐(PFOS)暴露对胎鼠肺部损伤的诱导作用,孕期SD大鼠在5～20mg·kg-1剂量范围内的PFOS中处理7d,取胎鼠全肺并分析其发育所受的影响。通过形态学比较,发现随着PFOS浓度的增加,胎鼠体长和体重均显著降低,高剂量暴露会导致胎鼠死亡。通过组织学检测,发现胎鼠肺的发育受到PFOS暴露的抑制。通过WesternBlot检测肺泡Ⅰ/Ⅱ型细胞的发育,发现肺泡Ⅰ型细胞特异蛋白Podoplanin表达显著减少(p<0.05),肺泡Ⅱ型细胞特异蛋白SP-C表达减少但未出现显著差异,此外,与对照组相比高剂量暴露会引起血管内皮生长因子(VEGF)表达显著减少(p<0.01)。实验结果说明,PFOS暴露会导致胎鼠肺部发育出现损伤,这种损伤可能是肺泡Ⅰ型细胞及肺部血管发育受抑制引起胎鼠肺部气体交换功能破坏。

关键词(KeyWords)： 全氟辛烷磺酸盐;胎鼠;肺泡细胞;血管内皮生长因子;发育毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(81102149-H2607);; 黑龙江省卫生厅科研课题项目(2010-235,2010-238);; 牡丹江医学院科学技术研究项目(2011-16)

作者(Author): 张春雷;李洪志;刘洁婷;包海花;吴丹;初彦辉;赵冰海;

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