镉诱导大鼠肾细胞凋亡及其机理的研究Study on the Mechanisms of NRK Cells Apoptosis Induced by Cadmium
贾广乐;王众;吴绍强;林祥梅;韩雪清;刘建;梅琳;张旻;
摘要(Abstract)：
为探讨镉(Cd)致大鼠肾细胞(NRK cell)凋亡的死亡受体途径,分别将终浓度为0、10、20、40、60μmol·L-1的CdCl2添加到培养液中,对NRK细胞暴露6h.应用流式细胞仪检测NRK细胞凋亡率;分光光度计检测Caspase-8蛋白活性;半定量RT-PCR检测Caspase-8、Fas的mRNA表达.结果表明,随着CdCl2暴露浓度的升高,NRK细胞凋亡率升高,Caspase-8蛋白活性增强,Caspase-8、Fas的mRNA表达增强,并呈现剂量-效应关系.说明镉可以通过死亡受体这条通路来引起NRK细胞凋亡.
关键词(KeyWords)： 镉;NRK细胞;死亡受体途径
基金项目(Foundation): 国家科研院所基本科研业务费资助项目(No. 2007JK022)
作者(Author): 贾广乐;王众;吴绍强;林祥梅;韩雪清;刘建;梅琳;张旻;
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参考文献(References)：
- Eichler T,Ma Q,Kelly C,Mishra J,Parikh S,Ransom R F,Devarajan P,Smoyer W E.2006.Single and combination toxic metal exposures induce apoptosis in cultured murine podocytes exclusively via the extrinsic caspase8pathway[J].Toxicological Sciences,90:392-399
- Friberg L,Elinder C G,Kjellstr-m T,Nordberg G F.1986.Cadmium and Healthy:A Toxicological and Epidemiological Approach[M].Boca Raton,FL:CRC
- Ishido M,Homma-Takeda S,Tohyama C,Suzuki T.1998.Apoptosis in rat renal proximal tubular cells induced by cadmium[J].Journal of Toxicology and Environmental Health Part A,55:1-12
- Lee W K,Abouhamed M,Thevenod F.2006.Caspase-dependent and-independent pathways for cadmium-induced apoptosis in cultured kidney proximal tubule cells[J].American Journal of Physiology-Renal Physiology,291:823-832
- Liu G W,Gong S L.2004.Progress study on signal transmission of apoptosis[J].Journal of Jilin University(Medicine Edition),30:485-488(in Chinese)
- Liu Y,Templeton D M.2007.Cadmium activates CaMK-Ⅱand initiates CaMK-Ⅱ-dependent apoptosis in mesangial cells[J].FEBS Letters,581:1481-1486
- Liu Z Q,Zhan J M.2006.Effect of cadmium on early apoptosis ofNRK cells and gene expression of bax[J].China Occupational Medicine,33:190-192(in Chinese)
- Mao W P,Ye J L,Guan Z B,Zhao J M Zhang C,Zhang N N,Jiang P,Tian T.2007.Cadmium induces apoptosis in human embryonic kidney(HEK)293cells by caspase-dependent and-independent pathways acting on mitochondria[J].Toxicology in Vitro,21:343-354
- Pham T N,Marion M,Denizeau F,Jumarie C.2006.Cadmium-induced apoptosis in rat hepatocytes does not necessarily involve caspase-dependent pathways[J].Toxicology in Vitro,20:1331-1342
- Thévenod F,Friedmann J M,Katsen A D,Hauser I A.2000.Up-regulation of multidrug resistance P-glycoprotein via nuclear factor-kappaB activation protects kidney proximal tubule cells from cadmium-and reactive oxygen species-induced apoptosis[J].The Journal of Biological Chemistry,275:1887-1896
- Wang W Z,Xu Z F,Yang J H,Pei X C.2004.Effect of cadmium on apoptosis of renal cells in mice[J].Chinese Journal of Industrial Medicine,17:4-6(in Chinese)
- 刘光伟,龚守良.2004.细胞凋亡信号传导途径的研究进展[J].吉林大学学报(医学版),30:485-488
- 刘占旗,战景明.2006.镉诱导大鼠肾细胞早期凋亡及bax基因表达的实验研究[J].中国职业医学,33:190-192
- 刘占旗,战景明,杨涛,窦岩.2005.氯化镉诱导大鼠肾细胞系细胞凋亡的研究[J].中国公共卫生,21:184-185