谭丽超;程燕;朱昱璇;卜元卿;周军英;单正军;

• 1:环境保护部南京环境科学研究所
• 2:南京信息工程大学江苏省大气环境与装备技术协同创新中心

摘要(Abstract)：

呋虫胺作为新一代烟碱类农药,究竟会给蜜蜂造成何种影响尚不清楚。本研究选取意大利蜜蜂为研究对象,分别测定呋虫胺对蜜蜂的急性毒性、慢性毒性以及幼虫发育毒性,并通过其危害商值(hazard quotient,HQ)初步评价呋虫胺对蜜蜂的生态风险,综合评价呋虫胺对蜜蜂的安全性。结果表明,急性经口毒性48 h半数致死剂量(48 h-LD50)为0.033μg·蜂-1,对蜜蜂高毒;慢性毒性10 d半数致死剂量(10 d-LDD50)为0.010μg·蜂-1,慢性毒作用带比值为3.5,存在慢性中毒的风险;幼虫7 d半数致死剂量为577 ng·幼虫-1,高剂量呋虫胺对蜜蜂幼虫存活率、化蛹率和羽化率均存在影响,处理剂量越高,存活率、化蛹率和羽化率越低,呈高度负相关(r=-0.98,-0.89,-0.80)。风险评价结果表明,呋虫胺对蜜蜂为中等风险到高风险。研究可为呋虫胺的合理使用提供科学参考。

关键词(KeyWords)： 呋虫胺;蜜蜂;急性毒性;慢性毒性;幼虫发育毒性

Abstract:

Keywords:

基金项目(Foundation): 中央级公益性科研院所2016年基本科研业务专项(农药环境污染防治监管制度研究);; 环境保护部农村与农业环境保护监管事业费项目

作者(Author): 谭丽超;程燕;朱昱璇;卜元卿;周军英;单正军;

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参考文献(References)：

• [1]代平礼,周婷,王强,等.吡虫啉对意大利蜜蜂学习行为的影响[J].农药,2013,52(7):512-514Dai P L,Zhou T,Wang Q,et al.Effects of imidacloprid on learning performance of Apis mellifera ligustica[J].Agrochemicals,2013,52(7):512-514(in Chinese)
• [2]Ollerton J,Winfree R,Tarrant S.How many flowering plants are pollinated by animals[J].Oikos,2011,120(3):321-326
• [3]Klein A M,Vaissière B E,Cane J H,et al.Importance of pollinators in changing landscapes for world crops[J].Proceedings Biological Sciences,2007,274(1608):303-313
• [4]Velthuis H H W,van Doorn A.A century of advances in bumblebee domestication and the economic and environmental aspects of its commercialization for pollination[J].Apidologie,2006,37(4):421-451
• [5]Garibaldi L A,Steffan-Dewenter I,Winfree R,et al.Wild pollinators enhance fruit set of crops regardless of honey bee abundance[J].Science,2013,339(6127):1608-1611
• [6]Gill R J,Ramos-Rodriguez O,Raine N E.Combined pesticide exposure severely affects individual-and colonylevel traits in bees[J].Nature,2012,491(7422):105-108
• [7]Farooqui T.A potential link among biogenic aminesbased pesticides,learning and memory,and colony collapse disorder:A unique hypothesis[J].Neurochemistry International,2013,62(1):122-136
• [8]陈秀.呋虫胺对映体对蜜蜂选择毒性及其在黄瓜和土壤中降解行为[D].北京:中国农业科学院,2013:43-44Chen X.Dinotefuran enantiomers:Enantioselective toxicities to honeybees and degradation behaviors in cucumber and soil[D].Beijing:Chinese Academy of Agricultural Sciences,2013:43-44(in Chinese)
• [9]姚香梅.新烟碱类杀虫剂对靶标和非靶标昆虫乙酰胆碱受体毒理学特性研究[D].北京:中国农业科学院,2015:47-59Yao X M.Pharmcological characteristics of neonicotinoid insecticides on nicotinic acetylcholine receptors of target and non-target insects[D].Beijing:Chinese Academy of Agricultural Sciences,2015:47-59(in Chinese)
• [10]Colin M E,Bonmatin J M,Moineau I,et al.A method to quantify and analyze the foraging activity of honey bees:Relevance to the sublethal effects induced by systemic insecticides[J].Archives of Environmental Contamination and Toxicology,2004,47(3):387-395
• [11]Henry M,Béguin M,Requier F,et al.A common pesticide decreases foraging success and survival in honey bees[J].Science,2012,336(6079):348-350
• [12]Williamson S M,Wright G A.Exposure to multiple cholinergic pesticides impairs olfactory learning and memory in honeybees[J].Journal of Experimental Biology,2013,216(Pt 10):1799-1807
• [13]国家质量监督检验检疫总局.GB/T 31270.10—2014化学农药环境安全评价试验准则第10部分:蜜蜂急性毒性试验[S].北京:中国标准出版社,2014
• [14]Kling A,Schmitzer S.Proposal for a new OECD guideline for the testing of chemical on adult honey bees(Apis mellifera L.)in a 10 day chronic feeding test in the laboratory and results of the recent ring test[C].Ghent,Belgium:12th International Symposium Hazards of Pesti-cides to Bees,International Symposium of the ICP-BR Bee Protection Group,2014
• [15]OECD.No.237,OECD guidelines for the testing of chemicals:Honey bee(Apis mellifera)larval toxicity test,single exposure[S].Paris,France:OECD,2013
• [16]谭丽超,卜元卿,程燕,等.吡虫啉对蜜蜂幼虫的室内毒性研究[J].生态毒理学报,2016,11(4):900-905Tan L C,Bu Y Q,Cheng Y,et al.Indoor toxicity research of imidacloprid on honeybee larvae[J].Asian Journal of Ecotoxicology,2016,11(4):900-905(in Chinese)
• [17]丁中海,王喆,潘国隆,等.联苯胺对大型溞(Daphnia magna)的急性和慢性毒性试验[J].应用与环境生物学报,2005,11(1):52-54Ding Z H,Wang Z,Pan G L,et al.Acute and chronic toxicities of benzidine to Daphnia magna[J].Chinese Journal of Applied and Environmental Biology,2005,11(1):52-54(in Chinese)
• [18]季守民,程传英,袁传卫,等.7种新烟碱类杀虫剂对意大利蜜蜂的急性毒性及风险评价[J].农药,2015,54(4):282-285Ji S M,Cheng C Y,Yuan C W,et al.Acute toxicity and risk assessment of seven neonicotinoid insecticides on honeybees(Apis mellifera L.)[J].Agrochemicals,2015,54(4):282-285(in Chinese)