海狗油脂肪乳对大鼠围产期毒性试验的研究The Toxic Effects of Seal Oil Emulsion on Rats during Perinatal Period
林飞;于燕;梁怿;周立娜;徐康森;
摘要(Abstract)：
观察了海狗油脂肪乳对大鼠妊娠晚期、分娩期、哺乳期及胚胎和胎仔出生后生长发育、学习能力以及生殖能力的影响.于大鼠妊娠D15至哺乳D21,连续皮下注射给予250、500和1000mg·kg-1剂量的海狗油脂肪乳,同时设0.9%氯化钠注射液为对照组,观察各组大鼠和胎仔的生长、发育、生殖能力等项指标.结果表明:给药后F0代母鼠体重增长明显减慢,摄食量显著减少,未见生殖、胚胎毒性反应;F1代仔鼠的出生体重和哺乳期体重增长减慢,生理达标、新生反射等发育迟缓;停药后F1代大鼠的体重增长、摄食量、脏器系数、同笼交配率、妊娠率及胚胎发育全过程未见明显生殖、发育等毒性变化.由此结论,海狗油脂肪乳可使母鼠和F1代仔鼠体重增长缓慢,生理发育迟缓,但不影响F1代仔鼠器官发育、生殖能力以及停药后体重增长和摄食量.
关键词(KeyWords)： 海狗油脂肪乳;大鼠;仔鼠;生长发育;生殖毒性
基金项目(Foundation):
作者(Author): 林飞;于燕;梁怿;周立娜;徐康森;
Email:
DOI:
参考文献(References)：
- Cao C P,Huang Z N,Yan D P,Shi M H.1997.Perinataltoxicity strudy of antibiotic89-07[J].Chinese Journal of Antibiotics,22(5):377-381(in Chinese)
- Fang L,Zhang J M,Yu H.2004.Influence of aiyincuiru liquidon the development of young puerperal mice[J].Drug Evaluation,1(4):287-289(in Chinese)
- Jiang H,Jiang Z M,Cai W,Tao Y X,Wang X R.2007.Emulsion[J].Chinese Journal of Clinical Nutrition,15(1):17-19(in Chinese)
- Li H,Lin F,Liang Y,Xu K S.2008.Teratogenicity of seal oilemulsion in rats[J].Carcinogenesis,Teratogenesis&Mutagenesis,20(6):477-480(in Chinese)
- Li Z J,Lin F,Le J J,Yan A Z,Xu K S.2003.Inhibitingeffect of seal oilω-3polyunsaturated fatty acids on experimentalbenign prostatic hyperplasia[J].Chinese Journal of PharmaceuticalAnalysis,23(3):176-180(in Chinese)
- Lin F,Liang Y,Song R X,Xu K S.2008.Examination of themutagenetic effect in the fat emulsion of seal oil[J].Journal ofPractical Oncology,22(4):313-316(in Chinese)
- Lin F,Yu Y,Liang Y,Hu S,Sun X.2007.The toxic effectsof ethaselen during perinatal period in rats[J].Carcinogenesis,Teratogenesis&Mutagenesis,19(4):320-323(in Chinese)
- Lin L,Xin Y,Xu K S.2002.Development of polyumsaturatedfatty acid from seal oil[J].Progress in Pharmaceutical Sciences,26(1):30-32(in Chinese)
- State Food and Drug Administration.2006.Guiding Principle forTechnology of Drug Reproductive Toxicity Study[S].Beijing:State Food and Drug Administration(in Chinese)
- Sun J.2005.Basis of Laboratory Animal Science[M].Beijing:Science&Technology Press,52-57(in Chinese)
- Yu J C,Guo W D.2006.Biological characteristics and researchstatus on fish oil emulsion[J].Journal of Clinical Surgery,14(4):243-245(in Chinese)
- Yu T L,Jun L G,Wu Z L,Wang J K.2007.Study on liverspleen and lung toxicity of the fat emulsion injection in rats[J].Pharmaceutical Journal of Chinese People’s Liberation Army,23(1):43-45(in Chinese)
- Yuan B J,Wang Z Q.1997.Preclinical safety evaluation andpractice of pharmaceuticals[J].Beijing:Military Medical Press,117-151(in Chinese)
- Zhou H J.2001.The International Technical Requirements ofDrug Registration-the Safety Part[M].Beijing:People’s MedicalPublishing House,87-113(in Chinese)
- 曹采蘋,黄正南,颜丹平,史民华.1997.抗生素89-07围产期毒性试验[J].中国抗生素杂志,22(5):377-381
- 方铝,张俊明,余华.2004.爱婴催乳液对围产期仔鼠生长、发育的影响[J].药品评价,1(4):287-289
- 国家食品药品监督管理局.2006.药物生殖毒性研究技术指导原则[S].北京:国家食品药品监督管理局
- 江华,蒋朱明,蔡威,陶晔璇,王秀荣.2007.脂肪乳[J].中国临床营养杂志,15(1):17-19
- 李华,林飞,梁怿,徐康森.2008.海狗油脂肪乳对大鼠致畸毒性研究[J].癌变.畸变.突变,20(6):477-480
- 李湛军,林飞,乐嘉静,阎安庄,徐康森.2003.海狗油ω-3多不饱和脂肪酸对实验性前列腺增生的抑制作用[J].药物分析杂志,23(3):176-180
- 林飞,梁怿,宋瑞霞,徐康森.2008.海狗油脂肪乳的致突变试验研究[J].实用肿瘤学杂志,22(4):313-316
- 林飞,于燕,梁怿,胡森科,孙晓英.2007.乙烷硒啉对大鼠围产期毒性的研究[J].癌变.畸变.突变,19(4):320-323
- 林兰,忻余,徐康森.2002.海狗油多烯脂肪酸的开发利用[J].药学进展,26(1):30-32
- 孙靖.2005.实验动物学基础[M].北京:北京科学技术出版社,52-57
- 于健春,郭卫东.2006.鱼油脂肪乳剂的生物学特性及其研究现状[J].临床外科杂志,14(4):243-245
- 喻涛,刘俊,李纲,吴子伦,王家葵.2007.脂肪乳注射液对大鼠肝脾肺的毒性研究[J].解放军药学学报,23(1):43-45
- 袁伯俊,王治乔.1997.新药临床前安全性评价与实践[M].北京:军事医科出版社,117-151
- 周海钧.2001.药品注册的国际技术要求(安全性部分)[M]北京:人民卫生出版社,87-113