16S rDNA克隆文库解析江汉平原高砷地下水系统中的细菌多样性Bacterial Diversity in High Arsenic Groundwater System of Jianghan Plain by 16S rDNA Clone Library
罗艳;谢作明;周义芳;王焰新;甘义群;
摘要(Abstract):
为研究江汉平原高砷地下水系统中细菌多样性和种群结构,以2011年11月采自江汉平原仙桃市沙湖镇高砷含水层沉积物为研究对象,沉积物采自同一50m钻井,分别选取埋深9.9~10.1m和15.7~16.0m样品。提取沉积物中总DNA,采用16SrDNA克隆文库方法,分析高砷含水层沉积物中土壤细菌的多样性。结果发现,伯克氏菌目(Burkholderiales)、假单胞杆菌目(Pseudomonadales)和肠杆菌目(Enterobacteriales)构成了高砷系统中抗砷的优势菌群。结果表明,高砷含水层中生活有大量微生物,这些微生物在砷的地球化学循环中扮演着重要角色。
关键词(KeyWords): 砷;微生物;细菌多样性
基金项目(Foundation): 国家自然科学基金(41172219);国家自然科学基金国际重大合作项目(41120124003);; 863计划项目(2012AA062602)
作者(Author): 罗艳;谢作明;周义芳;王焰新;甘义群;
Email:
DOI:
参考文献(References):
- [1]谢先军,王焰新,李俊霞,等.大同盆地高砷地下水稀土元素特征及其指示意义[J].地球科学(中国地质大学学报),2012,37(2):381-390Xie X J,Wang Y X,Li J X,et al.Characteristics and implications of rare earth elements in high arsenic groundwater from the Datong Basin[J].Earth Science(Journal of China University of Geosciences),2012,37(2):381-390(in Chinese)
- [2]World Health Organization.Guidelines for Drinking Water Quality.Recommendations[R].Geneva:World Health Or-ganization,2004
- [3]王国荃,吴顺华.地方性砷中毒的研究进展[J].新疆医科大学学报,2004,27(1):18-20
- [4]Rosado J L,Ronquillo D,Kordas K,et al.Arsenic expo-sure and cognitive performance in Mexican schoolchil-dren[J].Environmental Health Perspectives,2007115(9):1371-1375
- [5]赵伦山,武胜,周继华,等.大同盆地砷、氟中毒地方病生态地球化学研究[J].地学前缘,2007,14(2):225-235Zhao L S,Wu S,Zhou J H,et al.Eco-geochemical in-vestigation on the endemic As and F poisoning in Da-tong Basin[J].Earth Science Frontiers,2007,14(2)225-235(in Chinese)
- [6]何薪,马腾,王焰新,等.内蒙古河套平原高砷地下水赋存环境特征[J].中国地质,2010,37(3):781-788He X,Ma T,Wang Y X,et al.Geochemical characteris-tics of the As-bearing aquifer in the Hetao Plain,Inner Mongolia[J].Geology in China,2010,37(3):781-788(in Chinese)
- [7]罗艳丽,余艳华,郑春霞,等.新疆奎屯垦区土壤砷含量及耐砷植物的筛选[J].干旱区资源与环境,2010,24(2):192-194Luo Y L,Yu Y H,Zheng C X,et al.Arsenic concentra-tions in soils and plants in Kuitun Farm,XinJiang[J].Journal of Arid Land Resources and Environment,2010,24(2):192-194(in Chinese)
- [8]郁冬梅,吴学华,崔向红.从土壤地球化学看宁夏平罗县砷、氟中毒地方病的起因与分布[J].农业科学研究,2011,32(4):45-47Yu D M,Wu X H,Cui X H.Causes and distribution of endemic disease about arseniasis and fluorosis in Pingluo Country from a view of geochemistry[J].Journal of Agricultural Sciences,2011,32(4):45-47(in Chinese)
- [9]Lu K L,Liu C W,Wang S W,et al.Assessing the char-acteristics of groundwater quality of arsenic contamina-ted aquifers in the blackfoot disease endemic area[J].Journal of Hazardous Materials,2011,185(2-3):1458-1466
- [10]陈兴平,邓云华,张裕曾,等.湖北南洪村饮水砷含量及砷中毒调查[J].中国地方病防治杂志,2007,22(4):281-282Chen X P,Deng Y H,Zhang Y Z,et al.The epidemio-logical survey and analysis to arsenism in Nanhong Vil-lage of Plain Hubei Province[J].Chinese Journal of Control of Endemic Diseases,2007,22(4):281-282(in Chinese)
- [11]Huang S B,Wang Y X,Cao L,et al.Multidimensional spectrofluorometry characterization of dissolved organ-ic matter in arsenic-contaminated shallow groundwater[J].Journal of Environmental Science and Health,Part A:Toxic/Hazardous Substances and Environmental En-gineering2012,47(10):1446-1454
- [12]洪斌.微生物对砷的地球化学行为的影响———暨地下水砷污染机制的最新研究进展[J].地球科学进展,2006,21(1):77-82Hong B.Influence of microbes on biogeochemistry of arsenic—Mechanism of arsenic mobilization in ground-water[J].Advances in Earth Science,2006,21(1):77-82(in Chinese)
- [13]Oremland R S,Stolz J F.The ecology of arsenic[J].Science,2003,300(5621):939-944
- [14]唐小惠,郭华明,刘菲.富砷水环境中微生物及其环境效应的研究现状[J].水文地质工程地质,2008(3):104-107Tang X H,Guo H M,Liu F.Review of microbes and their effects in arsenic-rich waters[J].Hydrogeology and Engineering Geology,2008(3):104-107(in Chi-nese)
- [15]陈云嫩,柴立元.砷在地下水环境中的迁移转化[J].有色金属,2008,60(1):109-112Chen Y N,Chai L Y.Migration and transformation of arsenic in groundwater[J].Nonferrous Metals,2008,60(1):109-112(in Chinese)
- [16]Silver S,Phung L T.Genes and enzymes involved in bacterial oxidation and reduction of inorganic arsenic[J].Applied&Environmental Microbiology,2005,71(2):599-608
- [17]陈双喜,邵宗泽.西南印度洋中脊深海沉积物砷抗性菌的富集分离多样性分析[J].微生物学报,2008,48(10):1351-1355Chen S X,Shao Z Z.Enrichment and diversity analysis of arsenite-resistant bacteria in deep sea sediment sam-ples from southwest Indian Ocean ridge[J].Acta Mi-crobiologica Sinica,2008,48(10):1351-1355(in Chi-nese)
- [18]Yoshinaga M,Cai Y,Rosen B P.Demethylation of methylarsonicacid by a microbial community[J].Envi-ronmental Microbiology,2011,13(5):1205-1215
- [19]Oremland R S,Hoeft S E,Santini J A,et al.Anaerobic oxidation of arsenite in Mono Lake water and by facul-tative,arsenite-oxidizing chemoautotroph,strain ML-HE-1[J].Applied&Environmental Microbiology,2002,68(10):4795-4802
- [20]Rhine E D,Phelps C D,Young L Y.Anaerobic arsenite oxidation by novel denitrifying isolates[J].Environ-mental Microbiology,2006,8(5):899-908
- [21]Sun W J,Sierra R,Field J A.Anoxic oxidation of arse-nite linked to denitrification in sludges and sediments[J].Water Research,2008,42(17):4569-4577
- [22]Sun W J,Sierra-Alvarez R,Fernandez N,et al.Molecu-lar characterization and in situ quantification of anoxic arsenite-oxidizing denitrifying enrichment cultures[J].FEMS Microbiology Ecoligy,2009,68(1):72-85
- [23]Turpeinen R,Kairesalo T,H a¨ggblom M M.Microbial community structure and activity in arsenic-,chromi-um-and copper-contaminated soils[J].FEMS Microbi-ology Ecoligy,2004,47(1):39-50
- [24]Violante A,Huang P M,Gadd G M.Biophysico-Chemi-cal Processes of Heavy Metals and Metalloids in Soil Environments[M].Hoboken:Wiley,2008:313-338
- [25]Islam F S,Gault A G,Boothman C,et al.Role of met-al-reducing bacteria in arsenic release from Bengal delta sediments[J].Nature,2004,430:68-71
- [26]Chow S S,Taillefert M.Effect of arsenic concentration on microbial iron reduction and arsenic speciation in an iron-rich freshwater sediment[J].Geochimica et Cos-mochimica Acta,2009,73(20):6008-6021