王晓科;石清清;邓代莉;蒲生彦;

• 1:地质灾害防治与地质环境保护国家重点实验室(成都理工大学)
• 2:国家环境保护水土污染协同控制与联合修复重点实验室
• 3:香港理工大学土木及环境工程学系

摘要(Abstract)：

随着纳米科技与工业的高速发展,大量的纳米材料被广泛应用并最终汇聚到土壤环境中,对土壤生态和人体健康造成潜在影响。由于土壤生物具有多样性,选择具有代表性、敏感性并便于获取的土壤模式生物作为实验受体进行纳米材料的生物安全评估及环境毒理效应研究尤为重要。较为系统地回顾和总结了几种典型土壤模式生物的特点,为纳米材料毒理研究中受试生物的选择提供参考,在此基础上整理了大量基于典型土壤模式生物的纳米材料毒性研究资料,归纳了不同层次的研究方法,分析探索了纳米材料毒性机理,并展望了未来的研究重点。

关键词(KeyWords)： 纳米材料;模式生物;生物毒性;秀丽线虫;蚯蚓

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41772264);; 国家环境基准委托业务费择优资助项目(2016-BZ-003-W-17)

作者(Author): 王晓科;石清清;邓代莉;蒲生彦;

Email:

DOI:

参考文献(References)：

• [1]汪冰,丰伟悦,赵宇亮,等.纳米材料生物效应及其毒理学研究进展[J].中国科学:化学,2005,35(1):1-10
• [2]Service R F.Nanomaterials show signs of toxicity[J].Science,2003,300(5617):243
• [3]Masciangioli T,Zhang W X.Environmental technologies at the nanoscale[J].Environmental Science&Technology,2003,37(5):102A
• [4]张靖楠,李琪,梁文举.土壤线虫生态毒理学研究现状及展望[J].生态毒理学报,2009,4(3):305-314Zhang J N,Li Q,Liang W J.Present situation and prospect of soil nematode ecotoxicology[J].Asian Journal of Ecotoxicology,2009,4(3):305-314(in Chinese)
• [5]Choi J,Tsyusko O V,Unrine J M,et al.A micro-sized model for thein vivo study of nanoparticle toxicity:What has Caenorhabditis elegans taught us?[J].Environmental Chemistry,2014,11(3):227-246
• [6]张燕芬,王大勇.利用模式动物秀丽线虫建立环境毒物毒性的评估研究体系[J].生态毒理学报,2008,3(4):313-322Zhang Y F,Wang D Y.Establishment of toxicity evaluation system using model organism of Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2008,3(4):313-322(in Chinese)
• [7]沈敏,Coady K,董晶,等.化学品生态毒性测试鱼类模式生物的应用与展望[J].生态毒理学报,2017,12(2):34-43Shen M,Coady K,Dong J,et al.Application and outlook of various fish models used in chemical ecotoxicity test[J].Asian Journal of Ecotoxicology,2017,12(2):34-43(in Chinese)
• [8]陈晓雪,于振洋,尹大强.环境浓度水平的镍对不同生命阶段秀丽线虫(Caenorhabditis elegans)体长与运动的刺激效应[J].生态毒理学报,2014,9(2):299-305Chen X X,Yu Z Y,Yin D Q.Stimulations of nickel at environmental concentrations on locomotion and growth of Caenorhabditis elegans at different life stages[J].Asian Journal of Ecotoxicology,2014,9(2):299-305(in Chinese)
• [9]史志明,徐莉,胡锋.蚯蚓生物标记物在土壤生态风险评价中的应用[J].生态学报,2014,34(19):5369-5379Shi Z M,Xu L,Hu F.Progress in earthworm biomarker studies and theirs applications in soil pollution risk assessment[J].Acta Ecologica Sinica,2014,34(19):5369-5379(in Chinese)
• [10]徐冬梅,刘文丽,刘维屏.外源污染物对蚯蚓毒理作用研究进展[J].生态毒理学报,2009,4(1):21-27Xu D M,Liu W L,Liu W P.Research advances in toxicological effects of external pollutants on earthworms[J].Asian Journal of Ecotoxicology,2009,4(1):21-27(in Chinese)
• [11]Patricia C S,Nerea G V,Erik U,et al.Responses to silver nanoparticles and silver nitrate in a battery of biomarkers measured in coelomocytes and in target tissues of Eisenia fetida earthworms[J].Ecotoxicology and Environmental Safety,2017,141:57-63
• [12]保琼莉,李文华,黄益宗,等.土壤Cd污染对跳虫Folsomia candida的生态毒性[J].生态毒理学报,2017,12(2):169-176Bao Q L,Li W H,Huang Y Z,et al.Toxicity of Cd to springtails(Folsomia candida)in soil[J].Asian Journal of Ecotoxicology,2017,12(2):169-176(in Chinese)
• [13]苗秀莲,刘传栋,贾少波,等.中国5种土壤跳虫对重金属镍的毒性响应[J].生态毒理学报,2017,12(1):268-276Miao X L,Liu C D,Jia S B,et a1.Toxicity responses of five species of Chinese soil Collembola to Ni2+[J].Asian Journal of Ecotoxicology,2017,12(1):268-276(in Chinese)
• [14]张倩倩,乔敏,蔡爱芳,等.五氯酚对土壤跳虫代谢转化酶基因和蜕皮相关基因表达的影响[J].生态毒理学报,2017,12(5):72-78Zhang Q Q,Qiao M,Cai A F,et a1.Effects of pentachlorophenol on expression of metabolic enzyme genes and molt-related genes of soil Collembolan[J].Asian Journal of Ecotoxicology,2017,12(5):72-78(in Chinese)
• [15]Novak S,Drobne D,Menard A.Prolonged feeding of terrestrial isopod(Porcellio scaber,Isopoda,Crustacea)on Ti O2nanoparicles.Absence of toxic effect[J].Zookeys,2012,176:261-273
• [16]Lesly T B,Verbal O J.Caenorhabditis elegans,a biological model for research in toxicology[J].Reviews of Environmental Contamination&Toxicology,2016,237:1-35
• [17]王春花,李朝品,何梅.乐果对秀丽隐杆线虫生活史特征的影响[J].生态毒理学报,2015,10(2):332-337Wang C H,Li C P,He M.Effects of dimethoate on lifecycle traits of Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2015,10(2):332-337(in Chinese)
• [18]郭肖颖,王磊,李敏,等.基于秀丽隐杆线虫的微量水样环境毒理学研究[J].生态毒理学报,2016,11(1):345-352Guo X Y,Wang L,Li M,et a1.Environmental toxicologystudy of trace water samples with Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2016,11(1):345-352(in Chinese)
• [19]Mitani S.Nematode,an experimental animal in the national bioresource project[J].Experimental Animals,2009,58(4):351-356
• [20]杨振东,王加生,唐莉莉,等.玉米赤霉烯酮暴露对秀丽线虫生殖系统的损伤作用[J].生态毒理学报,2016,11(6):171-176Yang Z D,Wang J S,Tang L L,et al.The reproductive toxic effects of zearalenone on Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2016,11(6):171-176(in Chinese)
• [21]高上吉,钟晓霞,刘婉莹,等.基于彗星实验的洛克沙胂对秀丽隐杆线虫胚胎细胞DNA损伤的研究[J].生态毒理学报,2016,11(3):167-172Gao S J,Zhong X X,Liu W Y,et al.Comet assay study on DNA damage of embryonic cells in Caenorhabditis elegans induced by roxarsone[J].Asian Journal of Ecotoxicology,2016,11(3):167-172(in Chinese)
• [22]梁爽,于振洋,尹大强.环境浓度下磺胺混合物对秀丽线虫(Caenorhabditis elegans)生长、饮食、抗氧化酶及其调控基因表达水平的影响[J].生态毒理学报,2015,10(4):88-95Liang S,Yu Z Y,Yi D Q.Effects of sulfonamide mixtures at environmental concentrations on growth,feeding,catalase activity and the gene expression levels of Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2015,10(4):88-95(in Chinese)
• [23]Chen P H,Hsiao K M,Chou C C.Molecular characterization of toxicity mechanism of single-walled carbon nanotubes[J].Biomaterials,2013,34(22):5661-5669
• [24]Kim S W,Nam S H,An Y J.Interaction of silver nanoparticles with biological surfaces of Caenorhabditis elegans[J].Ecotoxicology&Environmental Safety,2012,77:64-70
• [25]Zanni E,Bellis G D,Bracciale M P,et al.Graphite nanoplatelets and Caenorhabditis elegans:Insights from an in vivo model[J].Nano Letters,2012,12(6):2740-2744
• [26]Wu Q,Yin L,Li X,et al.Contributions of altered permeability of intestinal barrier and defecation behavior to toxicity formation from graphene oxide in nematode Caenorhabditis elegans[J].Nanoscale,2013,5(20):9934-9943
• [27]Zhao Y,Wu Q,Wang D.An epigenetic signal encoded protection mechanism is activated by graphene oxide to inhibit its induced reproductive toxicity in Caenorhabditis elegans[J].Biomaterials,2016,79:15-24
• [28]Li P,Xu T T,Wu S Y,et al.Chronic exposure to graphene-based nanomaterials induces behavioral deficits and neural damage in Caenorhabditis elegans[J].Journal of Applied Toxicology,2017,37(10):1140-1150
• [29]Zhao Y L,Wang X,Wu Q L,et al.Translocation and neurotoxicity of Cd Te quantum dots in RMEs motor neurons in nematode Caenorhabditis elegans[J].Journal of Hazardous Materials,2015,283:480-489
• [30]Kim S W,Kwak J,An Y J.Fluorescent approach for visually observing quantum dot uptake in living organisms[J].Chemosphere,2016,144:1763-1770
• [31]孔璐,张婷,王大勇,等.纳米二氧化硅对秀丽线虫的毒性作用研究[J].生态毒理学报,2011,6(6):655-660Kong L,Zhang T,Wang D Y,et a1.Toxicity of Si O2nanoparticles to Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2011,6(6):655-660(in Chinese)
• [32]Li Y X,Wang W,Wu Q L,et al.Molecular control of Ti O2-NPs toxicity formation at predicted environmental relevant concentrations by Mn-SODs proteins[J].Plo S One,2012,7(9):e44688
• [33]Khare P,Sonane M,Pandey R,et al.Adverse effects of Ti O2and Zn O nanoparticles in soil nematode,Caenorhabditis elegans[J].Journal of Biomedical Nanotechnology,2011,7(1):116-117
• [34]Fajardo C,SaccàM L,Costa G,et al.Impact of Ag and Al2O3nanoparticles on soil organisms:In vitro and soil experiments[J].Science of the Total Environment,2014,254(3):473-474
• [35]Li Y X,Yu S H,Wu Q L,et al.Chronic Al2O3-nanoparticle exposure causes neurotoxic effects on locomotion behaviors by inducing severe ROS production and disruption of ROS defense mechanisms in nematode Caenorhabditis elegans[J].Journal of Hazardous Materials,2012,219:221-230
• [36]Wu Q,Nouara A,Li Y,et al.Comparison of toxicities from three metal oxide nanoparticles at environmental relevant concentrations in nematode Caenorhabditis elegans[J].Chemosphere,2013,90(3):1123-1131
• [37]孙耀琴,申聪聪,葛源.典型纳米材料的土壤微生物效应研究进展[J].生态毒理学报,2016,11(5):2-13Sun Y Q,Shen C C,Ge Y.Review on microbiological effects of typical nanomaterials in soil ecosystem[J].Asian Journal of Ecotoxicology,2016,11(5):2-13(in Chinese)
• [38]Qu M,Li Y H,Wu Q L,et al.Neuronal ERK signaling in response to graphene oxide in nematode Caenorhabditis elegans[J].Nanotoxicology,2017,11(4):520-533
• [39]Zhao Y L,Zhi L T,Wu Q L,et al.p38 MAPK-SKN-1/Nrf signaling cascade is required for intestinal barrier against graphene oxide toxicity in Caenorhabditis elegans[J].Nanotoxicology,2016,10(10):1469-1479
• [40]Zhi L,Ren M,Qu M,et al.Wnt ligands differentially regulate toxicity and translocation of graphene oxide through different mechanisms in Caenorhabditis elegans[J].Scientific Reports,2016,6:39261
• [41]Ren M X,Zhao L,Lv X,et al.Antimicrobial proteins in the response to graphene oxide in Caenorhabditis elegans[J].Nanotoxicology,2017,11(4):578-590
• [42]Wu Q L,Zhou X F,Han X X,et al.Genome-wide identification and functional analysis of long noncoding RNAs involved in the response to graphene oxide[J].Biomaterials,2016,102:277-291
• [43]Zhao L,Wan H,Liu Q,et al.Multi-walled carbon nanotubes-induced alterations in micro RNA let-7 and its targets activate a protection mechanism by conferring a developmental timing control[J].Particle&Fibre Toxicology,2017,14(1):27
• [44]Zhou Y F,Wang Q,Song B,et al.A real-time documentation and mechanistic investigation of quantum dots-induced autophagy in live Caenorhabditis elegans[J].Biomaterials,2015,72:38-48
• [45]Wu Q,Zhi L,Qu Y,et al.Quantum dots increased fat storage in intestine of Caenorhabditis elegans by influencing molecular basis for fatty acid metabolism[J].Nanomedicine-Nanotechnology Biology and Medicine,2016,12(5):1175-1184
• [46]SaccàM L,Fajardo C,Costa G,et al.Integrating classical and molecular approaches to evaluate the impact of nanosized zero-valent iron(n ZVI)on soil organisms[J].Chemosphere,2014,104(3):184-189
• [47]孔璐,唐萌,王大勇,等.不同尺度纳米镍对秀丽线虫的发育毒性[J].生态毒理学报,2013,8(4):623-628Kong L,Tang M,Wang D Y,et a1.Developmental toxicity of two size of nickel nanopartieles to Caenorhabditis elegans[J].Asian Journal of Ecotoxicology,2013,8(4):623-628(in Chinese)
• [48]Luo X,Xu S,Yang Y,et al.A novel method for assessing the toxicity of silver nanoparticles in Caenorhabditis elegans[J].Chemosphere,2017,168:648-657
• [49]Zhao Y,Wu Q,Li Y,et al.Translocation,transfer,and in vivo safety evaluation of engineered nanomaterials in the non-mammalian alternative toxicity assay model of nematode Caenorhabditis elegans[J].RSC Advances,2012,3(17):5741-5757
• [50]罗洁文,黄玫英,殷丹阳,等.蚯蚓在土壤污染风险评价中的应用研究进展[J].江苏农业科学,2016,44(8):24-29
• [51]张倩倩,乔敏,池海峰.土壤生态毒性测试方法综述[J].生态毒理学报,2017,12(4):76-97Zhang Q Q,Qiao M,Chi H F.Overview of soil ecotoxicity tests[J].Asian Journal of Ecotoxicology,2017,12(4):76-97(in Chinese)
• [52]van der Ploeg M J C,Baveco J M,van der Hout A,et al.Effects of C60 nanoparticle exposure on earthworms(Lumbricus rubellus)and implications for population dynamics[J].Environmental Pollution,2011,159(1):198-203
• [53]Zhao S L,He L,Lu Y F,et al.The impact of modified nano-carbon black on the earthworm Eisenia fetida under turfgrass growing conditions:Assessment of survival,biomass,and antioxidant enzymatic activities[J].Journal of Hazardous Materials,2017,338:218-223
• [54]Brami C,Glover A R,Butt K R,et al.Effects of silver nanoparticles on survival,biomass change and avoidance behaviour of the endogeic earthworm Allolobophora chlorotica[J].Ecotoxicology&Environmental Safety,2017,141:64-69
• [55]Wilson S W A,Reinsch B C,Tsyusko O V,et al.Role of particle size and soil type in toxicity of silver nanoparticles to earthworms[J].Soil Science Society of America Journal,2011,75(2):365-377
• [56]Heckmann L H,Hovgaard M B,Sutherland D S,et al.Limit-test toxicity screening of selected inorganic nanoparticles to the earthworm Eisenia fetida[J].Ecotoxicology,2011,20(1):226-233
• [57]Liang J,Xia X,Zhang W,et al.The biochemical and toxicological responses of earthworm(Eisenia fetida)following exposure to nanoscale zerovalent iron in a soil system[J].Environmental Science&Pollution Research,2017,24(3):1-8
• [58]Coleman J G,Johnson D R,Stanley J K,et al.Assessing the fate and effects of nano aluminum oxide in the terrestrial earthworm,Eisenia fetida[J].Environmental Toxicology&Chemistry,2010,29(7):1575-1580
• [59]Mcshane H,Sarrazin M,Whalen J K,et al.Reproductiveand behavioral responses of earthworms exposed to nanosized titanium dioxide in soil[J].Environmental Toxicology&Chemistry,2012,31(1):184-193
• [60]Khalil A M.Neurotoxicity and biochemical responses in the earthworm Pheretima hawayana,exposed to Ti O2NPs[J].Ecotoxicology&Environmental Safety,2015,122:455-461
• [61]Ca1as J E,Qi B,Li S,et al.Acute and reproductive toxicity of nano-sized metal oxides(Zn O and Ti O2)to earthworms(Eisenia fetida)[J].Journal of Environmental Monitoring,2011,13(12):3351-3357
• [62]刘玉荣,贺纪正,郑袁明.跳虫在土壤污染生态风险评价中的应用[J].生态毒理学报,2008,3(4):323-330Liu Y R,He J Z,Zheng Y M.A review of application of springtails in ecological risk assessment of contaminated soils[J].Asian Journal of Ecotoxicology,2008,3(4):323-330(in Chinese)
• [63]Tourinho P S,Gestel C A M V,Lofts S,et al.Metalbased nanoparticles in soil:Fate,behavior,and effects on soil invertebrates[J].Environmental Toxicology&Chemistry,2012,31(8):1679-1692
• [64]Waalewijn-Kool P L,Ortiz M D,Lofts S,et al.The effect of p H on the toxicity of zinc oxide nanoparticles to Folsomia candida in amended field soil[J].Environmental Toxicology&Chemistry,2013,32(10):2349-2355
• [65]Waalewijn-Kool P L,Klein K,Forniés R M,et al.Bioaccumulation and toxicity of silver nanoparticles and silver nitrate to the soil arthropod Folsomia candida[J].Ecotoxicology,2014,23(9):1629-1637
• [66]Novak S,Drobne D,Valant J,et al.Internalization of consumed Ti O2nanoparticles by a model invertebrate organism[J].Journal of Nanomaterials,2012,2012:1-8
• [67]Drobne D,Jemec A,Tkalec Z P.In vivo screening to determine hazards of nanoparticles:Nanosized Ti O2[J].Environmental Pollution,2009,157(4):1157-1164
• [68]Jemec A,Drobne D,Remskar M,et al.Effects of ingested nano-sized titanium dioxide on terrestrial isopods(Porcellio scaber)[J].Environmental Toxicology&Chemistry,2008,27(9):1904-1914