酸胁迫对铜藻早期生长阶段的影响Effect of Acid Stress on the Early Life Stages of Sargassum horneri
陈自强;孙庆海;陈全震;孙建璋;曾江宁;
摘要(Abstract):
为探究低pH胁迫对大型藻类早期生长的影响,以铜藻Sargassum horneri为研究对象,设置pH=3.5、4.5、5.5、6.5处理组和自然海水对照组,以不同处理时间分别对铜藻孢子和幼体进行胁迫实验。孢子实验中,于铜藻孢子附着前、后对其进行15、30、60、120 min的酸胁迫,以附着率和脱落率表征低pH对孢子附着的影响;幼体实验中,持续将铜藻幼体暴露于酸性条件下,以脱落死亡率表征pH对幼体的致死效应。结果显示,在短时间酸胁迫下,酸度对铜藻孢子附着的影响显著,附着率随pH值的下降而降低;胁迫时间对孢子的附着影响显著,附着率随胁迫时间的延长而降低,pH值与胁迫时间无交互作用;而当孢子已充分附着后,短期酸胁迫并没有显著影响孢子体的存活。在长时间酸胁迫下,与对照组相比,所有处理组铜藻幼体的存活率均显著降低。研究表明,低pH会对铜藻孢子、幼体的存活生长产生不利影响,并且这种影响在铜藻早期发育的不同阶段有所不同。
关键词(KeyWords): 低pH;铜藻;孢子;附着;幼体;早期发育
基金项目(Foundation): 国家“973”项目(2010CB428903);; 浙江省海水养殖重点科技创新团队(2010R50025-6);; 浙江省海洋环保项目“温州海域大型海藻资源调查与藻类资源恢复示范”;; 国家海洋局第二海洋研究所基本科研业务专项(JG0920)
作者(Author): 陈自强;孙庆海;陈全震;孙建璋;曾江宁;
Email:
DOI:
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