全氟辛酸(PFOA)对菲律宾蛤仔体内酶活性的影响The Effects of Perfluorooctanoic Acid(PFOA) on Enzyme Activities in Ruditapes philippinarum
刘芝余;翟毓秀;姚琳;江艳华;李风铃;王联珠;尚德荣;杨元昊;郭萌萌;谭志军;
摘要(Abstract):
采用半静态毒性实验方法,将菲律宾蛤仔(Ruditapes philippinarum)分别暴露于0.2、2、20μg·L-1的全氟辛酸(perfluorooctanoic acid,PFOA)中,在处理后第1、3、6、10、15、21天分别取样,测定整体组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽-S-转移酶(GST)、7-乙氧基异吩噁唑酮脱乙基酶(EROD)活性和过氧化脂质(LPO)含量。酶活性分析结果显示:PFOA对菲律宾蛤仔组织SOD、CAT和POD活性均呈现先促进后抑制的作用;低浓度组SOD活性在暴露第1天达到最高,显著高于对照组(P<0.01);中高浓度组SOD活性在暴露第6天达到最低;暴露1~15 d,低浓度组CAT活性均显著高于对照组(P<0.05);高浓度组CAT活性在暴露第6天得到显著诱导,其余时间基本处于抑制状态;中浓度组POD活性在暴露第3天即达到最高,高浓度组POD活性基本一直处于抑制状态;随着PFOA暴露时间的延长,菲律宾蛤仔组织LPO含量呈现了先降低后升高的趋势;各浓度组中EROD的活力都显著被诱导(P<0.01),与处理浓度呈正相关;中高浓度组的GST活性在胁迫期间变化比较显著,呈现诱导-抑制的变化规律。研究表明,PFOA暴露能够引起菲律宾蛤仔组织抗氧化酶和生物转化酶的变化,可以与其他敏感性指标一起作为指示早期海洋PFOA污染的生物标志物。
关键词(KeyWords): PFOA;菲律宾蛤仔;毒性效应;抗氧化酶系;生物转化酶
基金项目(Foundation): 山东省自然科学基金项目(ZR2014DQ025);; 国家科技基础性工作专项(2014FY230100);; 陕西省水利科技计划项目(2015SLKJ-22)
作者(Author): 刘芝余;翟毓秀;姚琳;江艳华;李风铃;王联珠;尚德荣;杨元昊;郭萌萌;谭志军;
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