刘芝余;翟毓秀;姚琳;江艳华;李风铃;王联珠;尚德荣;杨元昊;郭萌萌;谭志军;

• 1:中国水产科学研究院黄海水产研究所农业部水产品质量安全检测与评价重点实验室
• 2:南京农业大学无锡渔业学院
• 3:陕西省水产研究所中国水产科学研究院黄河水产研究所

摘要(Abstract)：

采用半静态毒性实验方法,将菲律宾蛤仔(Ruditapes philippinarum)分别暴露于0.2、2、20μg·L-1的全氟辛酸(perfluorooctanoic acid,PFOA)中,在处理后第1、3、6、10、15、21天分别取样,测定整体组织中超氧化物歧化酶(SOD)、过氧化氢酶(CAT)、过氧化物酶(POD)、谷胱甘肽-S-转移酶(GST)、7-乙氧基异吩噁唑酮脱乙基酶(EROD)活性和过氧化脂质(LPO)含量。酶活性分析结果显示:PFOA对菲律宾蛤仔组织SOD、CAT和POD活性均呈现先促进后抑制的作用;低浓度组SOD活性在暴露第1天达到最高,显著高于对照组(P<0.01);中高浓度组SOD活性在暴露第6天达到最低;暴露1~15 d,低浓度组CAT活性均显著高于对照组(P<0.05);高浓度组CAT活性在暴露第6天得到显著诱导,其余时间基本处于抑制状态;中浓度组POD活性在暴露第3天即达到最高,高浓度组POD活性基本一直处于抑制状态;随着PFOA暴露时间的延长,菲律宾蛤仔组织LPO含量呈现了先降低后升高的趋势;各浓度组中EROD的活力都显著被诱导(P<0.01),与处理浓度呈正相关;中高浓度组的GST活性在胁迫期间变化比较显著,呈现诱导-抑制的变化规律。研究表明,PFOA暴露能够引起菲律宾蛤仔组织抗氧化酶和生物转化酶的变化,可以与其他敏感性指标一起作为指示早期海洋PFOA污染的生物标志物。

关键词(KeyWords)： PFOA;菲律宾蛤仔;毒性效应;抗氧化酶系;生物转化酶

Abstract:

Keywords:

基金项目(Foundation): 山东省自然科学基金项目(ZR2014DQ025);; 国家科技基础性工作专项(2014FY230100);; 陕西省水利科技计划项目(2015SLKJ-22)

作者(Author): 刘芝余;翟毓秀;姚琳;江艳华;李风铃;王联珠;尚德荣;杨元昊;郭萌萌;谭志军;

Email:

参考文献(References)：

• [1]Prevedouros K,Cousins I T,Buck R C,et al.Sources,fate and transport of perfluorocarboxylates[J].Environmental Science&Technology,2006,40(1):32-44
• [2]Paul A G,Jones K C,Sweetman A J.A first global production,emission,and environmental inventory for perfluorooctane sulfonate[J].Environmental Science&Technology,2009,43(2):386-392
• [3]Shoeib M,Harner T,Webster G M,et al.Indoor sources of poly-and perfluorinated compounds(PFCS)in Vancouver,Canada:Implications for human exposure[J].Environmental Science&Technology,2011,45(19):7999-8005
• [4]Boulanger B,Vargo J,Schnoor J L,et al.Detection of perfluorooctane surfactants in great lakes water[J].Environmental Science&Technology,2004,38(15):4064-4070
• [5]Post G B,Louis J B,Lippincott R L,et al.Occurrence of perfluorinated compounds in raw water from New Jersey public drinking water systems[J].Environmental Science&Technology,2013,47(23):13266-13275
• [6]Lam N H,Cho C R,Lee J S,et al.Perfluorinated alkyl substances in water,sediment,plankton and fish from Korean rivers and lakes:A nationwide survey[J].Science of the Total Environment,2014,491-492:154-162
• [7]Route W T,Russell R E,Lindstrom A B,et al.Spatial and temporal patterns in concentrations of perfluorinatedcompounds in bald eagle nestlings in the upper Midwestern United States[J].Environmental Science&Technology,2014,48(12):6653-6560
• [8]Zhou Z,Shi Y,Vestergren R,et al.Highly elevated serum concentrations of perfluoroalkyl substances in fishery employees from Tangxun Lake,China[J].Environmental Science&Technology,2014,48(7):3864-3874
• [9]Zhang Y,Beesoon S,Zhu L,et al.Biomonitoring of perfluoroalkyl acids in human urine and estimates of biological half-life[J].Environmental Science&Technology,2013,47(18):10619-10627
• [10]Olsen G W,Burris J M,Ehresman D J,et al.Half-life of serum elimination of perfluorooctanesulfonate,perfluorohexanesulfonate,and perfluorooctanoate in retired fluorochemical production workers[J].Environmental Health Perspectives,2007,115(9):1298-1305
• [11]胡存丽,仲来福.全氟辛烷磺酸和全氟辛酸毒理学研究进展[J].中国工业医学杂志,2006,19(6):354-358Hu C L,Zhong L F.Progress of toxicological study on PFOS and PFOA[J].Chinese Journal of Industrial Medicine,2006,19(6):354-358(in Chinese)
• [12]周启星,胡献刚.PFOS/PFOA环境污染行为与毒性效应及机理研究进展[J].环境科学,2007,28(10):2153-2162Zhou Q X,Hu X G.Researching progresses in environmental pollution behavior,toxic effects and mechanisms of PFOS/PFOA[J].Environmental Science,2007,28(10):2153-2162(in Chinese)
• [13]金一和,汤先伟,曹秀娟,等.全球性全氟辛烷磺酰基化合物环境污染及其生物效应[J].自然杂志,2002,24(6):344-348Jin Y H,Tang X W,Cao X J,et al.Global environmental pollution and biological effects of perfluorooctane sulfonate[J].Chinese Journal of Nature,2002,24(6):344-348(in Chinese)
• [14]肖国强,张炯明,邵艳卿,等.苯并芘B[a]P对泥蚶组织EROD、GST酶活力和MDA含量的影响[J].海洋科学,2013,37(8):28-34Xiao G Q,Zhang J M,Shao Y Q,et al.Effect of benzo[a]pyrene on two enzymes activity and lipid peroxidation MAD in Tegillarca granosa[J].Marine Sciences,2013,37(8):28-34(in Chinese)
• [15]杨涛,陈海刚,蔡文贵,等.翡翠贻贝内脏团抗氧化酶活性及脂质过氧化物含量对苯并(b)荧蒽胁迫的生物响应研究[J].生态毒理学报,2011,6(5):539-545Yang T,Chen H G,Cai W G,et al.Response of antioxidant enzymes activities and lipid peroxidation levels in visceral mass of green-lipped mussel(Perna viridis)to benzo[b]fluoranthene stress[J].Asian Journal of Ecotoxicology,2011,6(5):539-545(in Chinese)
• [16]郭勤单,王有基,吕为群.温度和盐度对褐牙鲆幼鱼渗透生理及抗氧化水平的影响[J].水生生物学报,2014,38(1):58-67Guo Q D,Wang Y G,Lv W Q.Combined effects of temperature and salinity on the physiological osmotic induction and antioxidant responses in the juvenile Japanse flounder(Paralichthys olivaceus)[J].Acta Hydrobiologica Sinica,2014,38(1):58-67(in Chinese)
• [17]Winston G W,Giulio R T D.Prooxidant and antioxidant mechanisms in aquatic organisms[J].Aquatic Toxicology,1991,19(2):137-161
• [18]于红瑶,刘利,刘薇,等.PFOS致大鼠肝脏氧化损伤及对脂褐质含量影响[J].中国公共卫生,2009,25(5):578-579Yu H Y,Liu L,Liu W,et al.Oxidative damage of perfluorooctane sulfonate on lipofuscin levels in rat livers[J].Chinese Journal of Public Health,2009,25(5):578-579(in Chinese)
• [19]孙云之,刘缠民.全氟辛烷磺酰基化合物对无齿蚌鳃保护酶系的影响[J].安徽农业科学,2010,38(21):11150-11151Sun Y Z,Liu C M.Effect of perfloorooctane sulfonate on the protective enzyme activities in the gill of Anodonta woodiana[J].Journal of Anhui Agricultural Sciences,2010,38(21):11150-11151(in Chinese)
• [20]方雪梅,王建设,戴家银.全氟类有机污染物的污染状况及其生态毒理研究进展[J].地球科学进展,2010,25(5):543-551Fang X M,Wang J S,Dai J Y.The progress in environmental distributions and toxicological effects of perfluoroalkyl acids[J].Advance in Earth Science,2010,25(5):543-551(in Chinese)
• [21]Beyer J,Sandvik M,Hylland K,et al.Contaminant accumulation and biomarker responses in flounder(Platichthys flesus L.)and Atlantic cod(Gadus morhua L.)exposed by caging to polluted sediments in Srfjorden,Norway[J].Aquatic Toxicology,1996,36(1-2):75-98
• [22]Kopecka-Pilarczyk J,Correia A D.Biochemical response in gilthead seabream(Sparus aurata)to in vivo exposure to a mix of selected PAHs[J].Journal of Experimental Marine Biology&Ecology,2009,72(4):1296-1302
• [23]王淑红,王新红,陈荣,等.荧蒽、菲、芘对菲律宾蛤仔(Ruditapes philippinarum)超氧化物歧化酶的影响[J].厦门大学学报:自然科学版,2000,39(4):504-508Wang S H,Wang X H,Chen R,et al.The Effects of fluoranthene,phenaphthene and pyrene on superoxide dis-mutase(SOD)of Ruditapes philippinarum[J].Journal of Xiamen University:Natural Science,2000,39(4):504-508(in Chinese)
• [24]胡莹莹,李爱,叶赛,等.氯霉素对菲律宾蛤仔的急性和亚急性毒性作用[J].中国环境科学,2006,26(z1):125-128Hu Y Y,Li A,Ye S,et al.The acute and subacute toxicity action of chloramphenicol on Ruditapes philippinarum[J].China Environmental Science,2006,26(z1):125-128(in Chinese)
• [25]王琳,潘鲁青,许超群,等.Cd2+-B[α]P复合污染对菲律宾蛤仔急性毒性和解毒代谢酶活力的影响[J].水生生物学报,2011,35(1):37-44Wang L,Pan L Q,Xu C Q,et al.Combination effects of cadmium and benzo[α]pyrene on acute toxicity and metabolic enzymes activities of Ruditapes philippinarum[J].Acta Hydrobiologica Sinica,2011,35(1):37-44(in Chinese)
• [26]Hannam M L,Bamber S D,Moody J A,et al.Immune function in the Arctic Scallop,Chlamys islandica,following dispersed oil exposure[J].Aquatic Toxicology,2009,92(3):187-194
• [27]唐学玺,张培玉.蒽对黑鮶超氧化物歧化酶活性的影响[J].水产学报,2000,24(3):217-220Tang X X,Zhang P Y.Effects of anthracene on activity of superoxide dismutase in Sebastodes fuscescens[J].Journal of Fisheries of China,2000,24(3):217-220(in Chinese)
• [28]Stebbing A R.Hormesis--The stimulation of growth by low levels of inhibitors[J].Science of the Total Environment,1982,22(3):213-234
• [29]王贺威,马胜伟,张喆,等.全氟辛烷磺酸盐(PFOS)胁迫对翡翠贻贝抗氧化酶的影响[J].生态毒理学报,2012,7(5):508-516Wang H W,Ma S W,Zhang Z,et al.Effects of perfluorooctanesulfonate(PFOS)exposure on antioxidant enzymes of Perna viridis[J].Asian Journal of Ecotoxicology,2012,7(5):508-516(in Chinese)
• [30]于庆云,王悠,徐彦,等.镉和铅对菲律宾蛤仔脂质过氧化及抗氧化酶活性的影响[J].生态毒理学报,2013,8(4):504-512Yu Q Y,Wang Y,Xu Y,et al.Effects of cadmium and lead on the lipid peroxidation and levels of antioxidant enzymes in Ruditapes philippinarum[J].Asian Journal of Ecotoxicology,2013,8(4):504-512(in Chinese)
• [31]秦洁芳,陈海刚,蔡文贵,等.邻苯二甲酸二乙基己酯(DEHP)胁迫下红鳍笛鲷不同组织生化指标的变化[J].农业环境科学学报,2011,30(3):409-415Qin J F,Chen H G,Cai W G,et al.The change of biochemical indexes in different tissue of crimson snapper(Lutjanus erythropterus)exposed to di-(2-ethylhexyl)phthalate(DEHP)[J].Journal of Agro-Environment Science,2011,30(3):409-415(in Chinese)
• [32]Porte C,SoléM,Albaigés J,et al.Responses of mixedfunction oxygenase and antioxidant enzyme system of Mytilus sp.to organic pollution[J].Comparative Biochemistry&Physiology Part C:Comparative Pharmacology&Toxicology,1991,100(1-2):183-186
• [33]Richardson B J,Mak E,De Luca-Abbott S B,et al.Antioxidant responses to polycyclic aromatic hydrocarbons and organochlorine pesticides in green-lipped mussels(Perna viridis):Do mussels"integrate"biomarker responses?[J].Marine Pollution Bulletin,2008,57(6-12):503-514
• [34]Habig W H,Pabst M J,Jakoby W B.Glutathione Stransferases.The first enzymatic step in mercapturic acid formation[J].Journal of Biological Chemistry,1974,249(22):7130-7139
• [35]Bradford M M.A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding[J].Analytical Biochemistry,1976,72(1-2):248-254
• [36]贾守菊,应雪萍,陈艳乐,等.中华绒蟹不同发育阶段腹肢粘液腺同工酶的比较[J].海洋湖沼通报,2004(4):52-60Jia S J,Ying X P,Chen Y L,et al.A study on isozymes changes during veriant development periods of Eriocheir sinensis[J].Transaction of Oceanology&Limnology,2004(4):52-60(in Chinese)
• [37]张喆,马胜伟,王贺威.十溴联苯醚(BDE-209)对菲律宾蛤仔外套膜抗氧化酶活性的影响[J].生态学杂志,2013,32(1):122-128Zhang Z,Ma S W,Wang H W.Effects of decabromodiphenyl ether(BDE-209)on the antioxidant enzyme activities of Lutjanus argentimaculatus mantle membrane[J].Chinese Journal of Ecology,2013,32(1):122-128(in Chinese)
• [38]Lewis D F,Ioannides C,Parke D V.Cytochromes P450and species differences in xenobiotic metabolism and activation of carcinogen[J].Environmental Health Perspectives,1998,106(10):633-641
• [39]Shirani M,Mirvaghefi A,Farahmand H,et al.Biomarker responses in mudskipper(Periophthalmus waltoni)from the coastal areas of the Persian Gulf with oil pollution[J].Environmental Toxicology and Pharmacology,2012,34(3):705-713
• [40]孙虎山,李光友.栉孔扇贝血淋巴中超氧化物歧化酶和过氧化氢酶活性及其性质的研究[J].海洋与湖沼,2000,31(3):259-265Sun H S,Li G Y.Activities and properties of superoxide dismutase and catalase in the haemolymph of Chlamys farreri[J].Oceanologia et Limnologia Sinica,2000,31(3):259-265(in Chinese)
• [41]王贺威,马胜伟,张喆,等.全氟辛烷磺酸对真鲷鳃抗氧化酶和组织损伤的影响[J].生态与农村环境学报,2013,29(1):98-105Wang H W,Ma S W,Zhang Z,et al.Effects of perfluorooctane sulfonate(PFOS)on antioxidant enzyme in the gill of Pagrosomus major and damage to its tissues[J].Journal of Ecology and Rural Environment,2013,29(1):98-105(in Chinese)
• [42]洪美玲,陈立侨,顾顺樟,等.不同温度胁迫方式对中华绒蟹免疫化学指标的影响[J].应用与环境生物学报,2007,13(6):818-822Hong M L,Chen L Q,Gu S Z,et al.Effect of temperature change on immunochemical indexes of Eriocheir sinensis[J].Chinese Journal of Applied&Environmental Biology,2007,13(6):818-822(in Chinese)
• [43]Martínez-lvarez R M,Morales A E,Sanz A.Antioxidant defenses in fish:Biotic and abiotic factors[J].Reviews in Fish Biology and Fisheries,2005,15(1-2):75-88
• [44]Shin H S,Yoo J H,Min T S,et al.The effects of quercetin on physiological characteristics and oxidative stress resistance in olive flounder,Paralichthys olivaceus[J].Asian-Australasian Journal of Animal Sciences,2010,23(5):588-597
• [45]Viarengo A,Canesi L,Martinez P G,et al.Pro-oxidant processes and antioxidant defence systems in the tissues of the Antarctic scallop(Adamussium colbecki)compared with the Mediterranean scallop(Pecten jacobaeus)[J].Comparative Biochemistry&Physiology Part B:Biochemistry&Molecular Biology,1995,111(1):119-126
• [46]任加云,苗晶晶,李学平,等.石油烃暴露对栉孔扇贝(Chlamys farreri)组织生物转化酶及DNA损伤的影响[J].海洋湖沼通报,2016(3):46-54Ren J Y,Miao J J,Li X P,et al.Effects of petroleum hydrocarbon exposure on biotransformation enzymes activities and DNA damage of scallop Chlamys farreri[J].Transaction of Oceanology&Limnology,2016(3):46-54(in Chinese)
• [47]丁秀蓉,李正炎,王波,等.壬基酚对牙鲆肝脏EROD和GST酶活性的影响[J].中国海洋大学学报:自然科学版,2007(s1):101-104Ding X R,Li Z Y,Wang B,et al.Effects of nonylphenol on EROD and GST activity in the liver of Paralichthys olivaceus[J].Periodical of Ocean University of China,2007(s1):101-104(in Chinese)
• [48]王静,潘鲁青,苗晶晶.多环芳烃化合物对栉孔扇贝组织芳烃羟化酶活力的影响[J].海洋科学,2007,31(12):19-23Wang J,Pan L Q,Miao J J.Effects of polycyclic aromatic hydrocarbons on aryl hydrocarbon hydroxylase activities of marine scallop Chlamys farreri[J].Marine Sciences,2007,31(12):19-23(in Chinese)
• [49]费来华,邵雁群,李赟.栉孔扇贝不同组织SOD、CAT和MPO活力的比较分析[J].海洋湖沼通报,2008(3):128-132Fei L H,Shao Y Q,Li Y.Comparitive analysis of the activity of SOD,CAT and MPO in different tissues of the scallop Chlamys farreri[J].Transactions of Oceanology&Limnology,2008(3):128-132(in Chinese)
• [50]苗晶晶,潘鲁青,王静.苯并[a]芘对栉孔扇贝(Chlamys farreri)组织显微和超微结构的影响[J].环境科学学报,2007,27(9):1497-1503Miao J J,Pan L Q,Wang J.Effects of B[a]P on micro and ultrastructure of gill and digestive gland of Chlamys farreri[J].Acta Scientiae Circumstantiae,2007,27(9):1497-1503(in Chinese)
• [51]Fitzpatrick P J,Sheehan D,Livingstone D R.Studies on isoenzymes of glutathione S-transferase in the digestive gland of Mytilus galloprovincialis with exposure to pollution[J].Marine Environmental Research,1995,39(1-4):241-244
• [52]Wang C,Zhao Y,Zheng R,et al.Effects of tributyltin,benzo[a]pyrene,and their mixture on antioxidant defense systems in Sebastiscus marmoratus[J].Ecotoxicology and Environmental Safety,2006,65(3):381-387
• [53]穆景利,王新红,林建清,等.苯并[a]芘对黑鲷肝脏GST活性的影响及其与肝脏代谢酶和胆汁代谢产物之间的变化关系[J].生态毒理学报,2009,4(4):516-523Mu J L,Wang X H,Lin J Q,et al.Effects of benzoapyrene exposure on hepatic GST activity in black porgy(Sparus macrocephalus)and variation relationships with hepatic metabolic enzymes and biliary metabolites[J].Asian Journal of Ecotoxicology,2009,4(4):516-523(in Chinese)
• [54]王重刚,郑微云,余群,等.苯并(a)芘和芘的混合物暴露对梭鱼肝脏抗氧化酶活性的影响[J].环境科学学报,2002,22(4):529-533Wang C G,Zheng W Y,Yu Q,et al.Effects of mixture of benzo(a)pyrene and pyrene exposure on hepatic antioxidant enzymes activities in Mugil soiuy[J].Acta Scientiae Circumstantiae,2002,22(4):529-533(in Chinese)