五元氨基甲酸酯类农药混合物体系对青海弧菌的毒性特点Toxicity Characterstics of Five-Carbamate Pesticide Mixture System towards Vibrio qinghaiensis sp. Q67
张瑾;董欣琪;陈敏;周睿;桂荣洁;
摘要(Abstract):
以5种氨基甲酸酯类农药涕灭威(ALD)、残杀威(BAY)、呋喃丹(CAR)、灭多威(MET)和抗蚜威(PIR)为研究对象,应用均匀设计射线法设计五元混合物体系共6条射线(U1,U2,…,U6),应用基于发光菌青海弧菌Q67的微板毒性分析法(MTA)系统地考察了5种农药及其混合物的毒性,以浓度加和(CA)为参考模型分析混合物毒性相互作用(协同或拮抗作用)。结果表明,Logti和Weibull函数能较好地拟合5种氨基甲酸酯农药及其混合物对发光菌Q67的浓度-效应数据(R2>0.99,RMSE<0.032);以EC50的负对数值pEC50为毒性指标,5种农药的毒性顺序为BAY(pEC50=2.87)>CAR(pEC50=2.67)>ALD(pEC50=2.00)>MET(pEC50=1.99)>PIR(pEC50=1.79);依据CA,五元氨基甲酸酯类农药的6条混合物射线中,有2条呈加和作用,4条呈拮抗作用,其中U2和U4在整条浓度-效应曲线上呈现了明显的拮抗作用,而U3和U6的弱拮抗作用分别发生在混合物浓度的中高浓度区和中低浓度区;五元氨基甲酸酯类农药混合物的毒性与组分灭多威(MET)的浓度比呈良好的负相关关系(r=-0.9238),且线性模型对混合物毒性具有良好的预测能力。
关键词(KeyWords): 氨基甲酸酯类农药;青海弧菌;毒性特点;拮抗作用;线性模型
基金项目(Foundation): 国家自然科学基金(No.21677001,21207002);; 安徽省自然科学基金(No.1708085MB50);; 国家级大学生科技创新项目
作者(Author): 张瑾;董欣琪;陈敏;周睿;桂荣洁;
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