毒死蜱、马拉硫磷和氰戊菊酯对赤子爱胜蚓(Eisenia foetida)的急性毒性Acute Toxicological Dosages of Chlorpyrifos, Malathion and Fenvalerate on Earthworm Eisenia foetida
刘伟;朱鲁生;王军;谢慧;王金花;
摘要(Abstract):
采用滤纸接触法和土壤培养法研究了毒死蜱(Chlorpyrifos)、马拉硫磷(Malathion)和氰戊菊酯(Fenvalerate)对赤子爱胜蚓(Eisenia foetida)的急性毒性效应.滤纸接触法试验浓度分别设置为,毒死蜱:3.14、6.28、9.42、12.56、15.70、18.84μg·cm-2;马拉硫磷:3.10、6.20、12.40、18.60、24.80、31.40μg·cm-2;氰戊菊酯:0.31、0.62、1.24、1.86、2.48、3.14μg·cm-2.土壤培养法试验浓度分别设置为,毒死蜱:100、125、150、175、200、225、250mg·kg-1;马拉硫磷:350、400、450、500、550、600mg·kg-1;氰戊菊酯:20、30、40、50、60、70、80mg·kg-1.结果表明:采用滤纸接触法测得的各农药对蚯蚓48h的半数致死浓度(LC50)分别为毒死蜱12.26μg·cm-2、马拉硫磷19.61μg·cm-2、氰戊菊酯1.03μg·cm-2;采用土壤培养法测得的各农药对蚯蚓7d的LC50分别为毒死蜱427.67mg·kg-1、马拉硫磷742.53mg·kg-1、氰戊菊酯81.81mg·kg-1,14d的LC50分别为毒死蜱182.21mg·kg-1、马拉硫磷497.29mg·kg-1、氰戊菊酯37.46mg·kg-1.根据《化学农药环境安全评价试验准则》,这3种农药对蚯蚓的毒性均为低毒级.
关键词(KeyWords): 毒死蜱;马拉硫磷;氰戊菊酯;赤子爱胜蚓;急性毒性
基金项目(Foundation): 国家自然科学基金项目(No.20477022;No.40801203)
作者(Author): 刘伟;朱鲁生;王军;谢慧;王金花;
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DOI:
参考文献(References):
- Arnaud C,Saint-Denis M,Narbonne J F,Soler P,Ribera D.2000.Influences of different standardised test methods onbiochemical responses in the earthworm Eisenia fetida andrei[J].Soil Biology&Biochemistry,32(1):67-73
- Cortez J,BouchéM.2001.Decomposition of mediterranean leaflitters by Nicodrilus meridionalis(Lumbricidae)in laboratory andfield experiments[J].Soil Biology and Biochemistry,33(15):2023-2035
- Gao M L,Dai S G,Zhang P.2006.Single and binary-combined toxicity of chlorotoluron and atrazine on earthworm[J].Ecology and Environment,15(3):525-528(in Chinese)
- Institute for the Control of Agrochemicals,Ministry of Agriculture,The People’s of Republic of China.2004.Experimental Criterionfor Environmental Safety Assessment of Chemical Pesticide[Z](in Chinese)
- Li S Y,Zhang X K,Feng J F,Sun C,Wang L S.2002.Influence of fenvalerate and its metabolites on catalase activity insoil[J].China Environmental Science,22(2):154-157(inChinese)
- Li Y S,Zeng Z L,Chen Z L,Qiu J P.2004.Acute toxicityof veterinary pharmaceutical-roxarsone to earthworms[J].Journalof Shanghai Jiaotong University(Agricultural Science),22(2):115-119(in Chinese)
- Liang J D,Zhou Q X.2003.Single and binary-combinedtoxicity of methamidophos,acetochlor and Cu on earthwormEisenia foetida[J].Chinese Journal of Applied Ecology,14(4):593-596(in Chinese)
- Liu W.2006.Study on the Toxic Effect of Chlorpyrifos,Malathionand Fenvalerates on Earthworm and Broad Bean[D].Taian:Shandong Agricultural University(in Chinese)
- Luo Y,Zang Y,Zhong Yuan,Kong Z M.1999.Toxicologicalstudy of two novel pesticides on earthworm Eisenia foetida[J].Chemosphere,39(13):2347-2356
- McInerney M,Little D J,Bolger T.2001.Effect of earthwormcast formation on the stabilization of organic matter in fine soilfractions[J].European Journal of Soil Biology,37(4):251-254
- OECD.1984.OECD Guideline for Testing of Chemicals,No.207:Earthworm Acute Toxicity Tests[S].Paris:OECD
- Puurtinen H M,Martikainen E.1997.Effect of soil moisture onpesticide toxicity to an enchytraeid worm,enchytraeus sp.[J].Archives of Environmental Contamination and Toxicology,33(1):34-41
- Shi Y J,LüY L,Wang X,Zhang X.2009.Effects of lindaneand carbofuran on mortality,growth,and reproduction of earthworms(Eisenia foetida)[J].Asian Journal of Ecotoxicology,4(1):101-107(in Chinese)
- Zhang R W,Li Z X,Bai Q Y,Cai D J.1985.Application ofa standardized method for determining acute toxicity of somepesticides in earthworms(Eisenia foetida)[J].Acta ScientiaeCircumstantiae,3(3):327-333(in Chinese)
- Zhou S P,Duan C Q,Han Q H,Zhang Z W,Sun C Y,Hu YH.2005.Effect of chlorpyrifos on soil sucrase activity[J].Ecology and Environment,14(5):672-674(in Chinese)
- Zhu L S,Fan D F,Wang Y J.1999.Toxicity and evaluationof phoxim,fenpropathrin and their mixture to earthworm[J].Journal of Zhejiang Agricultural University,25(1):77-80(inChinese)
- Zhu L S,Xu Y X,Wang Y J,Li G D,Zhang Y F.2000.Effects of malathion,fenvalerate and its mixture on respiration ofsoil microbe[J].Journal of Shandong Agricultural University(Natural Science),31(1):35-37(in Chinese)
- Zuo H G,Lin Y S,Gong R Z.2005.Single and combinedtoxicity of carbofuran and dimehypo to earthworms[J].RuralEco-Environment,21(1):69-71(in Chinese)
- 高敏苓,戴树桂,张平.2006.绿麦隆、阿特拉津单一与复合污染对蚯蚓的毒性效应研究[J].生态环境,15(3):525-528
- 李时银,张晓昆,冯建昉,孙成,王连生.2002.氰戊菊酯及代谢物对土壤过氧化氢酶活性的影响[J].中国环境科学,22(2):154-157
- 李银生,曾振灵,陈杖榴,邱江平.2004.洛克沙胂对蚯蚓的急性毒性试验[J].上海交通大学学报(农业科学版),22(2):115-119
- 梁继东,周启星.2003.甲胺磷、乙草胺和铜单一与复合污染对蚯蚓的毒性效应研究[J].应用生态学报,14(4):593-596
- 刘伟.2006.毒死蜱、马拉硫磷和氰戊菊酯对蚯蚓、蚕豆毒性效应的研究[D].泰安:山东农业大学
- 农业部农药检定所.2004.化学农药环境安全评价试验准则[Z]
- 史雅娟,吕永龙,王昕,张翔.2009.林丹和呋喃丹对赤子爱胜蚓存活、生长和繁殖能力的影响[J].生态毒理学报,4(1):101-107
- 张壬午,李治祥,白清云,蔡道基.1985.应用标准方法测定农药对蚯蚓的毒性[J].环境科学学报,3(3):327-333
- 周世萍,段昌群,韩青辉,张正旺,孙超云,胡艳红.2005.毒死蜱对土壤蔗糖酶活性的影响[J].生态环境,14(5):672-674
- 朱鲁生,樊德方,王玉军.1999.辛硫磷、甲氰菊酯及其混剂对蚯蚓的毒性及安全性评价[J].浙江大学学报(农业与生命科学版),25(1):77-80
- 朱鲁生,徐玉新,王玉军,李光德,张玉风.2000.马拉硫磷、氰戊菊酯及其混剂对土壤微生物影响研究[J].山东农业大学学报(自然科学版),31(1):35-37
- 左海根,林玉锁,龚瑞忠.2005.呋喃丹、杀虫双对蚯蚓(Eisennia foetida)的单一和复合毒性[J].农村生态环境,21(1):69-71