重金属Cr(VI)、Pb及Cu胁迫对双齿围沙蚕体腔细胞的DNA损伤DNA Damage of Coelomocyte in Perinereis aibuhitensis Induced by Chromium( VI),Lead and Copper
张来军;陈永敢;杭瑜瑜;
摘要(Abstract):
为探讨重金属Cr(VI)、Pb以及Cu对沙蚕体腔细胞DNA的毒性效应,以双齿围沙蚕为受试动物,重金属按不同剂量水平,Cr(VI):10、100和200 mg·L~(-1),Pb:5、50和100 mg·L~(-1),Cu:1、10和20 mg·L~(-1),分别胁迫沙蚕24 h,以不加任何重金属离子的海水为对照,采用单细胞凝胶电泳技术,检测其体腔细胞DNA损伤程度。结果表明,与空白对照组相比,3种重金属离子的各浓度组都能引起沙蚕体腔细胞DNA损伤,且3种重金属胁迫浓度与细胞DNA损伤程度之间存在显著的剂量-效应关系。双齿围沙蚕可以作为单细胞凝胶电泳的实验材料用于重金属所致环境污染的生物监测指示生物。
关键词(KeyWords): 重金属;双齿围沙蚕;遗传毒性;DNA损伤;体腔细胞;彗星实验
基金项目(Foundation): 国家自然科学基金(31260139);; 海南省自然科学基金(313050)
作者(Author): 张来军;陈永敢;杭瑜瑜;
Email:
DOI:
参考文献(References):
- [1]唐博,龙江平,金路,等.珠江口和北部湾附近海域沉积物重金属生态风险比较[J].热带海洋学报,2015,34(3):75-81Tang B,Long J P,Jin L,et al.The contrast of heavy metals’ecological risk in marine sediments between the Beibu Gulf and the Pearl River Delta[J].Journal of Tropical Oceanography,2015,34(3):75-81(in Chinese)
- [2]蔡继翔,刘烨,梁明才,等.单细胞凝胶电泳应用研究进展[J].生物信息学,2016,14(2):95-99Cai J X,Liu Y,Liang M C,et al.Research progress about the application of single cell gel electrophoresis[J].Chinese Journal of Bioinformatics,2016,14(2):95-99(in Chinese)
- [3]卞方杰,邢美燕,杨健,等.彗星实验技术在水生与土壤环境中的毒理学应用[J].生态毒理学报,2015,10(3):63-70Bian F J,Xing M Y,Yang J,et al.The toxicologic applications of the comet assay in the aquatic and soil environment[J].Asian Journal of Ecotoxicology,2015,10(3):63-70(in Chinese)
- [4]Woo S,Kim S,Yum S,et al.Comet assay for the detection of genotoxicity in blood cells of flounder(Paralichthys olivaceus)exposed to sediments and polycyclic aromatic hydrocarbons[J].Marine Pollution Bulletin,2006,52(12):1768-1775
- [5]王晓艳,蒋凤华,冯丽娟,等.石油烃对栉孔扇贝血淋巴细胞DNA损伤的初步研究[J].生态毒理学报,2012,7(3):305-311Wang X Y,Jiang F H,Feng L J,et al.Effect of petroleum hydrocarbons on DNA damage of hemolymph cells inscallop Chlamys farreri:A preliminary research[J].Asian Journal of Ecotoxicology,2012,7(3):305-311(in Chinese)
- [6]SoléM,Kopecka-Pilarczyk J,Blasco J.Pollution biomarkers in two estuarine invertebrates,Nereis diversicolor and Scrobicularia plana,from a Marsh ecosystem in SW Spain[J].Environment International,2009,35(3):523-531
- [7]Pérez E,Blasco J,SoléM.Biomarker responses to pollution in two invertebrate species:Scrobicularia plana and Nereis diversicolor from the Cádiz bay(SW Spain)[J].Marine Environmental Research,2004,58(2-5):275-279
- [8]孙福红,周启星,张倩如.石油烃、Cu2+对沙蚕的毒性效应及对其抗氧化酶系统的影响[J].环境科学,2006,27(7):1415-1419Sun F H,Zhou Q X,Zhang Q R.Toxic effect of petroleum hydrocarbons and copper on polychaete Nereis diversicolor and on its antioxidant enzyme systems[J].Environmental Science,2006,27(7):1415-1419(in Chinese)
- [9]王晶,周启星,张倩茹,等.沙蚕暴露于石油烃、Cu2+和Cd2+毒性效应及乙酰胆碱酯酶活性的响应[J].环境科学,2007,28(8):1796-1801Wang J,Zhou Q X,Zhang Q R,et al.Toxic effects petroleum hydrocarbons,copper and cadmium on polychaete Perinereis aibuhitensis Grube and on its responses in acetycholinesterase activity[J].Environmental Science,2007,28(8):1796-1801(in Chinese)
- [10]Lewis C,Galloway T.Genotoxic damage in polychaetes:A study of species and cell-type sensitivities[J].Mutation Research,2008,654(1):69-75
- [11]Eyambe G S,Goven A J,Fitzpatrick L C,et al.A non-invasive technique for sequential collection of earthworm(Lumbricus terrestris)leukocytes during subchronic immunotoxicity studies[J].Laboratory Animals,1991,25:61-67
- [12]Zhang L J,Jia J F,Hao J G,et al.A modified protocol for the comet assay allowing the processing of multiple samples[J].Mutation Research,2011,721:153-156
- [13]田雨露.双齿围沙蚕(Perinereis aibuhitensis)的形态学研究及其对Pb2+的毒性响应[D].青岛:中国海洋大学,2014:1-8Tian Y L.Morphological study and toxic effects of Pb2+on polychaete Perinereis aibuhitensis[D].Qingdao:Ocean University of China,2014:1-8(in Chinese)
- [14]朱江.镉-菲复合污染对安德爱胜蚓(Eisenia andrei)和白线蚓(Fridericia bulbosa)的生态毒理效应研究[D].上海:上海交通大学,2008:92-97Zhu J.Study on ecotoxicological effects of combined pollution between cadmium and phenanthrene on earthworm(Eisenia andrei)and enchytraeid(Fridericia bulbosa)in soil[D].Shanghai:Shanghai Jiao Tong University,2008:92-97(in Chinese)
- [15]Le D0-Lacoste M,Akcha F,Dévier M H,et al.Comparative study of different exposure routes on the biotransformation and genotoxicity of PAHs in the flatfish species,Scophthalmus maximus[J].Environmental Science and Pollution Research International,2013,20(2):690-707
- [16]Bhagat J,Ingole B S.Genotoxic potency of mercuric chloride in gill cells of marine gastropod Planaxis sulcatus using comet assay[J].Environmental Science and Pollution Research International,2015,22(14):10758-10768
- [17]Dallas L J,Cheung V V,Fisher A S,et al.Relative sensitivity of two marine bivalves for detection of genotoxic and cytotoxic effects:A field assessment in the Tamar Estuary,South West England[J].Environmental Monitoring and Assessment,2013,185(4):3397-3412
- [18]Devi V J,Nagarani N,Babu M Y,et al.A study of proteotoxicity and genotoxicity induced by the pesticide and fungicide on marine invertebrate(Donax faba)[J].Chemosphere,2013,90(3):1158-1166
- [19]Devi V J,Nagarani N,Babu M Y,et al.Genotoxic effects of profenofos on the marine fish,Therapon jarbua[J].Toxicology Mechanisms and Methods,2012,22(2):111-117
- [20]Barranger A,Heude-Berthelin C,Rouxel J,et al.Parental exposure to the herbicide diuron results in oxidative DNA damage to germinal cells of the Pacific oyster Crassostrea gigas[J].Comparative Biochemistry and Physiology C:Toxicology&Pharmacology,2016,180:23-30
- [21]Nagarani N,Devi V J,Kumaraguru A K.Identification of DNA damage in marine fish Therapon jarbua by comet assay technique[J].Journal of Environmental Biology,2012,33(4):699-703
- [22]Al-Subiai S N,Moody A J,Mustafa S A,et al.A multiple biomarker approach to investigate the effects of copper on the marine bivalve mollusk,Mytilus edulis[J].Ecotoxicology and Environmental Safety,2011,74(7):1913-1920
- [23]Galloway T,Lewis C,Dolciotti I,et al.Sublethal toxicity of nano-titanium dioxide and carbon nanotubes in a sediment dwelling marine polychaete[J].Environmental Pollution,2010,158(5):1748-1755
- [24]Catalano B,Moltedo G,Martuccio G.Can Hediste diversicolor(Nereidae,Polychaete)be considered a good candidate in evaluating PAH contamination?A multimarker approach[J].Chemosphere,2012,86(9):875-882◆