成组生物毒性测试法综合评价典型工业废水毒性Synthetic Evaluation on Toxicity of Typical Industrial Effluents Using a Battery of Bioassays
邹叶娜;蔡焕兴;薛银刚;徐东炯;
摘要(Abstract):
为了更加准确地评估典型工业废水的综合生物毒性以及处理工艺对废水毒性的削减情况,采用发光细菌急性毒性实验、大型溞急性毒性实验和单细胞凝胶电泳实验,结合潜在生态毒性效应探测(potential ecotoxic effects probe,PEEP)指数对常州市7种典型工业废水的综合生物毒性进行了评价。结果表明,7种工业原水都表现出了急性毒性或遗传毒性,综合生物毒性强度的排序为电子厂>电镀厂>综合污水处理厂>印染厂>化工厂>食品厂>制药厂。而7种工业废水的处理后出水综合生物毒性强度的排序为印染厂>化工厂>电子厂>综合污水处理厂>食品厂>制药厂>电镀厂。其中,印染和化工厂出水综合生物毒性高于原水,分别增加了43.3%和38.7%,PEEP评价结果显示分别属于剧毒和高毒,而电镀、电子、综合污水处理和食品厂出水的综合生物毒性削减明显,分别比原水削减了76.9%、53.1%、48.3%和26.6%,PEEP结果表明基本无毒。建立在成组生物毒性实验基础上的PEEP评价方法可全面反映工业废水的综合生物毒性,进而更客观地评价废水对水生态系统乃至人类健康的潜在影响。
关键词(KeyWords): 成组生物毒性测试;工业废水;PEEP;发光细菌;大型溞;单细胞凝胶电泳
基金项目(Foundation): 江苏省环保科研课题(2009027),江苏省环保科研课题(2012065);; 江苏省环境监测科研基金项目(1106)
作者(Author): 邹叶娜;蔡焕兴;薛银刚;徐东炯;
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DOI:
参考文献(References):
- [1]Mankiewicz-Boczek J,Nalecz-Jawecki G,Drobniewska A,et al.Application of a microbiotests battery for complete toxicity assessment of rivers[J].Ecotoxicology and Environmental Safety,2008,71(3):830-836
- [2]Rao S S.Impact Assessment of Hazardous Aquatic Contaminants:Concepts and Approaches[M].Boca Raton:Lewis Publishers,1999:161-181
- [3]Blinova I.The perspective of microbiotests application to surface water monitoring and effluent control in Estonia[J].Environmental Toxicology,2000,15(5):385-389
- [4]Kaza M,Mankiewicz-Boczek J,Izydorczyk K,et al.Toxicity assessment of water samples from rivers in Central Poland using a battery of microbiotests—A pilot study[J].Polish Journal of Environmental Studies,2007,16(1):81-89
- [5]Blaise C,Férard J F.Small-Scale Freshwater Toxicity Investigations[M].Dordrecht:Springer Publishers,2005:115-137,233-255
- [6]Castillo G C,Vila I C,Neild E.Ecotoxicity assessment of metals and wastewater using multitrophic assays[J].Environmental Toxicology,2000,15(5):370-375
- [7]Blaise C,Kusui T.Acute toxicity assessment of industrial effluents with a microplate-based Hydra attenuata assay[J].Environmental Toxicology and Water Quality,1997,12(1):53-60
- [8]Birkholz D,Belton K,Guidotti T.Toxicological evaluation for the hazard assessment of tire crumb for use in public playgrounds[J].Journal of Air&Waste Management Association,2003,53(7):903-907
- [9]王茜,张辰佳,刘占峰,等.工业污水对多刺裸腹潘心率、体长和抗氧化酶系的影响[J].生态学杂志,2009,28(2):300-303Wang Q,Zhang C J,Liu Z F,et al.Effects of industrial wastewater on heart rate,body length and antioxidases of Moina macrocopa[J].Chinese Journal of Ecology,2009,28(2):300-303(in Chinese)
- [10]李丽君,刘振乾,徐国栋,等.工业废水的鱼类急性毒性效应研究[J].生态科学,2006,25(1):43-47Li L J,Liu Z Q,Xu G D,et al.Study on acute-toxicity of six kind industrial wastewaters to zebrafish[J].Journal of Ecological Science,2006,25(1):43-47(in Chinese)
- [11]陈莉.电催化氧化杀藻降解藻毒素中水质毒性变化的研究[D].北京:北京林业大学,2010:51-90Chen L.Study on toxicity variation of water quality during the electrocatalytic oxidation of algae and MCLR[D].Beijing:Beijing Forestry University,2010:51-90(in Chinese)
- [12]宋晓威,徐建,张孝飞,等.废弃农药厂污染场地浅层地下水生态毒性诊断研究[J].农业环境科学学报,2011,30(1):42-48Song X W,Xu J,Zhang X F,et al.Ecology toxicity diagnostic research on an abandoned pesticide factory contaminated site of shallow groundwater[J].Journal of Agro-Environment Science,2011,30(1):42-48(in Chinese)
- [13]Costan G,Bermingham N,Blaise C,et al.Potential ecotoxic effects probe(PEEP):A novel index to assess and compare the toxic potential of industrial effluents[J].Environmental Toxicology and Water Quality,1993,8(2):115-140
- [14]祝威,沈捷,黄翔峰,等.生物法/人工湿地工艺处理采油废水及其生态毒性削减研究[J].环境污染与防治,2009,31(11):53-57Zhu W,Shen J,Huang X F,et al.Treatment and ecotoxicity reduction of oilfield produced water by biological/constructed wetland process[J].Environmental Pollution and Control,2009,31(11):53-57
- [15]查金苗,王子健.利用日本青鳉早期发育阶段暴露评估排水的急、慢性毒性和内分泌干扰效应[J].环境科学学报,2005,25(12):1682-1686Zha J M,Wang Z J.Assessment acute and chronic toxicity and endocrine disruption effects of whole effluent based on early stage development of Japanese medaka(Oryzias latipes)[J].Acta Scientiae Circumstantiae,2005,25(12):1682-1686(in Chinese)
- [16]刘宛,李培军,周启星,等.环境污染条件下生物体内DNA损伤的生物标记物研究进展[J].应用与环境生物学报,2005,11(2):251-255Liu W,Li P J,Zhou Q X,et al.Research advances of biomarkers of DNA damage in organisms under environmental pollution[J].Chinese Journal of Applied and Environmental Biology,2005,11(2):251-255(in Chinese)
- [17]金小伟,査金苗,许宜平,等.3种氯酚类化合物对河蚬的毒性和氧化应激[J].生态毒理学报,2009,4(6):816-822Jin X W,Zha J M,Xu Y P,et al.Toxicity and oxidative stress of three chlorophenols to freshwater clam Corbicula fluminea[J].Asian Journal of Ecotoxicology,2009,4(6):816-822(in Chinese)
- [18]刘芸,李娜,马梅,等.酚类化合物雌激素效应的比较研究[J].中国环境科学,2009,29(8):873-878Liu Y,Li N,Ma M,et al.Comparative study of phenolic compounds estrogenic and anti-estrogenic activities[J].China Environmental Science,2009,29(8):873-878(in Chinese)
- [19]冯承莲,许宜平,何悦,等.十溴联苯醚(BDE209)在虹鳟体内的羟基代谢产物及其对甲状腺激素水平影响的初步研究[J].生态毒理学报,2010,5(3):327-333Feng C L,Xu Y P,He Y,et al.Preliminary research on hydroxylated metabolites of decabromodiphenyl ether and their effects on thyroid hormone in rainbow trout(Oncorhynchus mykiss)[J].Asian Journal of Ecotoxicology,2010,5(3):327-333(in Chinese)
- [20]高磊,李兆利,陈辉辉,等.染织排水对日本青鳉幼鱼和胚胎的毒性效应[J].生态毒理学报,2011,6(3):303-309Gao L,Li Z L,Chen H H,et al.Toxicity of dye effluent on Japanese medaka(Oryzias latipes)embryo and larava[J].Asian Journal of Ecotoxicology,2011,6(3):303-309(in Chinese)