马力;林治卿;张华山;闫竣;张伟;林本成;刘焕亮;张英鸽;袭著革;

• 1:军事医学科学院卫生学环境医学研究所
• 2:国家纳米科学中心军事医学科学院纳米药理毒理协作实验室

摘要(Abstract)：

为探讨纳米二氧化硅(Nano-SiO2)、纳米四氧化三铁(Nano-Fe3O4)和单壁碳纳米管(SWCNTs)对大鼠肝、肾的毒性效应,将49只雄性Wistar大鼠随机分为7组,包括生理盐水对照组,以及3种纳米材料的低剂量组(2mg·mL-1)和高剂量组(10mg·mL-1),采用非暴露式气管滴注法染毒,每2d染毒1次,每次每只0.2mL,共染毒5周,眼眶取血后处死大鼠,称肝、肾重量计算脏器系数,测定大鼠血清中反映肝、肾功能的生化指标,并对肝、肾进行病理学观察.结果表明,1)3种纳米材料均可导致大鼠体重明显降低,但对肝、肾脏器系数无明显影响;2)病理学观察发现,3种纳米材料均可导致大鼠肝细胞轻度脂肪变性,肾脏则无明显改变;3)3种纳米材料均可导致大鼠肝功能异常,部分肝功能指标如谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷丙转氨酶(ALT)等出现显著降低;4)3种纳米材料均可导致大鼠肾功能异常,部分肾功能指标如尿酸(UA)、肌酐(CREA)、尿素氮(BUN)等出现显著升高或降低.以上结果提示,经呼吸道染毒的Nano-SiO2、Nano-Fe3O4和SWCNTs均可对大鼠肝、肾产生一定的毒性效应.

关键词(KeyWords)： 纳米二氧化硅;纳米四氧化三铁;单壁碳纳米管;肝毒性;肾毒性

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.90406024);; 国家高技术研究发展规划(863)项目(No.2006AA03Z330)

作者(Author): 马力;林治卿;张华山;闫竣;张伟;林本成;刘焕亮;张英鸽;袭著革;

Email:

参考文献(References)：

• Borm P J,Robbins D,Haubold S,Kuhlbusch T,Fissan H,Donaldson K,Schins R,Stone V,Kreyling W,Lademann J,Krutmann J,Warheit D,Oberdrster E.2006.The potential risksof nanomaterials:a review carried out for ECETOC[J].Particleand Fibre Toxicology,3:11
• Brumfiel G.2003.Nanotechnology:A little knowledge[J].Nature,424:246-248
• Dreher K L.2004.Health and environmental impact of nanotech-nology:toxicological assessment of manufactured nanoparticles[J].Toxicological Sciences,77:3-5
• Khandoga A,Stampfl A,Takenaka S,Schulz H,Radykewicz R,Kreyling W,Krombach F.2004.Ultrafine particles exertprothrombotic but not inflammatory effects on the hepaticmicrocirculation in healthy mice in vivo[J].Circulation,109:1320-1325
• Kwon J T,Hwang S K,Jin H,Kim D S,Minai-Tehrani A,Yoon H J,Choi M,Yoon T J,Han D Y,Kang Y W,Yoon BI,Lee J K,Cho M H.2008.Body distribution of inhaledfluorescent magnetic nanoparticles in the mice[J].Journal ofOccupational Health,50:1-6
• Lewinski N,Colvin V,Drezek R.2008.Cytotoxicity of nanoparticles[J].Small,4:26-49
• Li N,Xia T,Nel A E.2008.The role of oxidative stress in ambientparticulate matter-induced lung diseases and its implications inthe toxicity of engineered nanoparticles[J].Free Radical Biology&Medicine,44:1689-1699
• Lin B C,Xi Z G,Zhang Y G,Zhang H S,Yang D F,Sun X,Zhang W,Liu H L.2007.Experimental study on the reproductivedamage of male rats induced by micro-nano-scale SiO2[J].Asian Journal of Ecotoxicology,2:195-201(in Chinese)
• Mller W,Felten K,Sommerer K,Scheuch G,Meyer G,MeyerP,Haussinger K,Kreyling W G.2008.Deposition,retentionand translocation of ultrafine particles from the central airwaysand lung periphery[J].American Journal of Respiratory andCritical Care Medicine,177:426-432
• Monteiro-Riviere N A,Nemanich R J,Inman A O,Wang Y Y,Riviere J E.2005.Multi-walled carbon nanotube interactionswith human epidermal keratinocytes[J].Toxicology Letters,155:377-384
• Oberdrster G,Oberdrster E,Oberdrster J.2005.Nanotoxicology:an emerging discipline evolving from studies of ultrafine particles[J].Environmental Health Perspectives,113:823-839
• Renwick L,Brown D,Clouter A,Donaldson K.2004.Increasedinflammation and altered macrophage chemotactic responses causedby two ultrafine particle types[J].Occupational&EnvironmentalMedicine,61:442-447
• Service R F.2003.American Chemical Society meeting.Nanomaterials show signs of toxicity[J].Science,300:243
• Shi J H,Wu X T,Fan M X,Jiang G R.1995.Toxication,distribution and excretion of Fe3O4ultramicron in mice and dogs[J].Chinese Journal of Physical Meicine and Rehabilitation,17:71-73(in Chinese)
• Wang H F,Wang J,Deng X Y,Sun H F,Shi Z J,Gu Z N,Liu Y F,Zhao Y L.2004.Biodistribution of carbon single-wallcarbon nanotubes in mice[J].Journal of Nanoscience andNanotechnology,4:1019-1024
• 林本成,袭著革,张英鸽,张华山,杨丹凤,孙欣,张伟,刘焕亮.2007.微纳尺度SiO2对雄性大鼠生殖功能损伤的实验研究[J].生态毒理学报,2:195-201
• 施建华,吴信陶,樊明信,姜光锐.1995.四氧化三铁超微粒子的毒性及其在小鼠体内的分布和排泄[J].中华物理医学杂志,17:71-73