非暴露式气管滴注3种典型纳米材料对大鼠肝、肾的毒性效应Hepatotoxicity and Nephrotoxicity of Rats Induced by Intratracheal Injection of Three Typical Nanomaterials
马力;林治卿;张华山;闫竣;张伟;林本成;刘焕亮;张英鸽;袭著革;
摘要(Abstract):
为探讨纳米二氧化硅(Nano-SiO2)、纳米四氧化三铁(Nano-Fe3O4)和单壁碳纳米管(SWCNTs)对大鼠肝、肾的毒性效应,将49只雄性Wistar大鼠随机分为7组,包括生理盐水对照组,以及3种纳米材料的低剂量组(2mg·mL-1)和高剂量组(10mg·mL-1),采用非暴露式气管滴注法染毒,每2d染毒1次,每次每只0.2mL,共染毒5周,眼眶取血后处死大鼠,称肝、肾重量计算脏器系数,测定大鼠血清中反映肝、肾功能的生化指标,并对肝、肾进行病理学观察.结果表明,1)3种纳米材料均可导致大鼠体重明显降低,但对肝、肾脏器系数无明显影响;2)病理学观察发现,3种纳米材料均可导致大鼠肝细胞轻度脂肪变性,肾脏则无明显改变;3)3种纳米材料均可导致大鼠肝功能异常,部分肝功能指标如谷草转氨酶(AST)、碱性磷酸酶(ALP)、谷丙转氨酶(ALT)等出现显著降低;4)3种纳米材料均可导致大鼠肾功能异常,部分肾功能指标如尿酸(UA)、肌酐(CREA)、尿素氮(BUN)等出现显著升高或降低.以上结果提示,经呼吸道染毒的Nano-SiO2、Nano-Fe3O4和SWCNTs均可对大鼠肝、肾产生一定的毒性效应.
关键词(KeyWords): 纳米二氧化硅;纳米四氧化三铁;单壁碳纳米管;肝毒性;肾毒性
基金项目(Foundation): 国家自然科学基金项目(No.90406024);; 国家高技术研究发展规划(863)项目(No.2006AA03Z330)
作者(Author): 马力;林治卿;张华山;闫竣;张伟;林本成;刘焕亮;张英鸽;袭著革;
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