多氯联苯对我国土壤微生物的生态毒理效应Ecotoxicity of Polychlorinated Biphenyls( PCBs) to Microorganisms in Chinese Udic-ferrosols and Aquic-cambosols
程金金;宋静;吕明超;王兴祥;
摘要(Abstract):
作为持久性有机污染物(POPs),多氯联苯(PCBs)一旦进入土壤将长期存留并对土栖生物产生潜在危害。土壤微生物是土壤生态系统重要组成部分,研究外源PCBs对土壤微生物的生态毒理效应,筛选出指示PCBs污染的敏感指标并获取可靠的生态毒理数据十分重要。研究以江西红壤和天津潮土为供试土壤,在室内25℃连续培养28 d的条件下进行了生态毒理实验,选择了微生物量碳、呼吸强度、代谢熵、硝化作用、脱氢酶活性、脲酶活性和微生物群落功能多样性为微生物指标。结果显示:1)在28 d培养时间内,多氯联苯(PCBs)的毒性作用随培养时间的延长而增强,且在红壤中的毒性作用强于在潮土中,表明PCBs对土壤微生物的毒性作用存在时间效应并受土壤性质的影响。2)各微生物指标的敏感性不同,微生物量碳、脲酶活性和微生物功能多样性对PCBs污染反应不够敏感,而土壤呼吸强度、代谢熵、硝化作用和脱氢酶活性对PCBs污染反应敏感。3)14 d时,红壤中PCBs对脱氢酶活性、呼吸强度和代谢熵的EC10值分别为1.20、3.18和1.09 mg·kg-1,而在潮土中分别为6.31、4.73和>50 mg·kg-1;28 d时,红壤中PCBs对硝化作用、脱氢酶活性、呼吸强度和代谢熵的EC10值分别为2.32、0.77、0.51和0.71mg·kg-1,而在潮土中分别为5.91、1.65、3.00和>50 mg·kg-1。综合考虑经济和实际需要等因素,建议将呼吸强度、硝化作用和脱氢酶活性作为PCBs污染土壤生态毒理评价中的首选敏感指标,并建议培养时间设置为28 d。
关键词(KeyWords): 多氯联苯(PCBs);红壤;潮土;土壤微生物;生态毒理
基金项目(Foundation): 环保公益性行业科研专项经费项目(201009032);; 国家高新技术研究发展计划(863计划)项目(2009AA063104)
作者(Author): 程金金;宋静;吕明超;王兴祥;
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参考文献(References):
- [1]杨佳佳,吴淑琪,佟玲.多氯联苯的环境特性及分析测试进展[J].岩矿测试,2009,28(5):444-451Yang J J,Wu S Q,Tong L.Environmental characteristics and the progress on analytical methods of polychlorinated biphenyls[J].Rock and Mineral Analysis,2009,28(5):444-451(in Chinese)
- [2]Chander K,Brookes P C.Microbial biomass dynamics following addition of metal-enriched sewage sludge to a sandy loam[J].Soil Biology and Biochemistry,1995,27(11):1409-1421
- [3]Renella G,Mench M,Gelsomin A,et al.Functional activity and microbial community structure in soil amended with bimetallic sludges[J].Soil Biology and Biochemistry,2005,37(8):1498-1506
- [4]Van Beelen P,Doelman P.Significance and application of microbial toxicity tests in assessing ecotoxicological risks of contaminants in soil and sediment[J].Chemosphere,1997,34(3):455-499
- [5]Anan'eva N D,Khakimov F I,Deeva N F,et al.The influence of polychlorinated biphenyls on the microbial biomass and respiration in gray forest soil[J].Eurasian Soil Science,2005,38(7):770-775
- [6]Dusek L,TesaˇrováM.Influence of polychlorinated biphenyls on microbial biomass and its activity in grassland soil[J].Biology and Fertility of Soils,1996,22(3):243-247
- [7]孙红斌,刘亚云,陈桂珠.PCBs对红树林沉积物中微生物及酶活性的影响[J].生态学报,2007,27(12):5398-5407Sun H B,Liu Y Y,Chen G Z.Effect of PCBs on microbial and enzyme activities in vegetated and non-vegetated mangrove sediments[J].Acta Ecologica Sinica,2007,27(12):5398-5407(in Chinese)
- [8]Ding N,Hayat T,Wang J,et al.Responses of microbial community in rhizosphere soils when ryegrass was subjected to stress from PCBs[J].Journal of Soils and Sediments,2011,11(8):1355-1362
- [9]Safe S.Toxicology,structure-function relationship,and human and environmental health impacts of polychlorinated biphenyls:Progress and problems[J].Environmental Health Perspectives,1993,100:259-268
- [10]王祥云,邓勋飞,杨洪达,等.金华城区土壤中7种指示性多氯联苯(PCBs)的分布特征和来源分析[J].农业环境科学学报,2012,31(8):1512-1518Wang X Y,Deng X F,Yang H D,et al.The distribution and source analysis of the seven indicator polychlorinated biphenyls(PCBs)in urban soils of Jinhua City,China[J].Journal of Agro-Environment Science,2012,31(8):1512-1518(in Chinese)
- [11]OECD.OECD Guidelines for the Testing of ChemicalsSoil Microorganisms:Nitrogen Transformation Test.OECD Method 216.[S].Paris:OECD,2000
- [12]OECD.OECD Guidelines for the Testing of ChemicalsSoil Microorganisms:Carbon Transformation Test.OECD Method 217.[S].Paris:OECD,2000
- [13]ISO.Soil Quality-Biological Methods-Determination of Nitrogen Mineralization and Nitrifi cation in Soils and the Infiuence of Chemicals on These Processes.ISO Method14238.[S].Geneva:ISO,1997
- [14]ISO.Soil Quality-Determination of Soil Microbial Biomass.ISO Method 14240.[S].Geneva:ISO,1997
- [15]ISO.Soil Quality-Determination of Potential Nitrification and Inhibition of Nitrification-Rapid Test by Ammonium Oxidation.ISO Method 15685.[S].Geneva:ISO,2012
- [16]ISO.Soil Quality-Laboratory Methods for Determination of Microbial Soil Respiration.ISO Method 16072.[S].Geneva:ISO,2002
- [17]ISO.Soil Quality-Determination of Dehydrogenase Activity in Soil.ISO Method 23753.[S].Geneva:ISO,2005
- [18]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 27854-2011,化学品-土壤微生物-氮转化试验[S].北京:中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会,2011
- [19]中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会.GB/T 27855-2011,化学品-土壤微生物-碳转化试验[S].北京:中华人民共和国国家质量监督检验检疫总局,中国国家标准化管理委员会,2011
- [20]Hoekstra J A,van Ewijk P H.Alternatives for the no-observed-effect level[J].Environmental Toxicology and Chemistry,1993,12(1):187-195
- [21]Pires A M,Branco J A,Picado A,et al.Models for the estimation of a"no effect concentration"[J].Environmetrics,2002,13(1):15-27
- [22]关松荫.土壤酶及其研究法[M].北京:农业出版社,1986:268-278
- [23]郑华,欧阳志云,方治国,等.BIOLOG在土壤微生物群落功能多样性研究中的应用[J].土壤学报,2004,41(3):456-461Zheng H,Ouyang Z Y,Fang Z G,et al.Application of BIOLOG to study on soil microbial community functional diversity[J].Acta Pedologica Sinica,2004,41(3):456-461(in Chinese)
- [24]高军,骆永明,滕应,等.多氯联苯污染土壤的微生物生态效应研究[J].农业环境科学学报,2009 28(2):228-233Gao J,Luo Y M,Teng Y,et al.Microbial ecological effects in soil contaminated with polychlorinated biphenyls[J].Journal of Agro-Environment Science,2009,28(2):228-233(in Chinese)
- [25]Deng S P,Tabatabai M A.Effect of tillage and residue management on enzyme activities in soils:III.Phosphatase and arylsulfatase[J].Biology and Fertility of Soils,1997,24(2):141-146
- [26]Klose S,Tabatabai M A.Arylsulfatase activity of microbial biomass in soils as affected by cropping systems[J].Biology and Fertility of Soils,1999,29(1):46-54
- [27]Li X Z,Sarah P.Arylsulfatase activity of soil microbial biomass along a Mediterranean-arid transect[J].Soil Biology and Biochemistry,2003,35(7):925-934
- [28]Bierkens J,Klein G,Corbisier P,et al.Comparative sensitivity of 20 bioassays for soil quality[J].Chemosphere,1998,37(14-15):2935-2947
- [29]Preston-Mafham J,Boddy L,Randerson P F.Analysis of microbial community functional diversity using sole-car-bon-source utilisation profiles-A critique[J].FEMS Microbiology Ecology,2002,42(1):1-14
- [30]陈承利,廖敏,曾路生.污染土壤微生物群落结构多样性及功能多样性测定方法[J].生态学报,2006,26(10):3404-3412Chen C L,Liao M,Zeng L S.Methods to measure the microbial community structure and functional diversity in polluted soils[J].Acta Ecologica Sinica,2006,26(10):3404-3412(in Chinese)
- [31]张文清,吕伟娇,陈强,等.不同分子量壳聚糖对土壤碳、氮及呼吸的影响[J].生态学报,2006,26(4):1280-1284Zhang W Q,Lv W J,Chen Q,et al.The effect of molecular weight of chitosan on soil’s carbon,nitrogen and soil respiration[J].Acta Ecologica Sinica,2006,26(4):1280-1284(in Chinese)
- [32]Anderson T H,Domsch K H.The metabolic quotient for CO2(qCO2)as a specific activity parameter to assess the effects of environmental conditions,such as pH,on the microbial biomass of forest soils[J].Soil Biology and Biochemistry,1993,25(3):393-395
- [33]吴宇澄,骆永明,滕应,等.多氯联苯污染农田土壤的细菌群落结构差异及其影响因素[J].土壤学报,2007,44(5):854-859Wu Y C,Luo Y M,Teng Y,et al.Variation of microbial communities in PCBs-contaminated agricultural soils and influencing factors[J].Acta Pedologica Sinica,2007,44(5):854-859(in Chinese)
- [34]夏月,朱永官.硝化作用作为生态毒性指标评价土壤重金属污染生态风险[J].生态毒理学报,2007,2(3):273-279Xia Y,Zhu Y G.Nitrification as ecotoxicological endpoint in risk assessment of soil heavy metal pollution[J].Asian Journal of Ecotoxicology,2007,2(3):273-279(in Chinese)
- [35]Duek L.Activity of nitrifying populations in grassland soil polluted by polychlorinated biphenyls[J].Plant and Soil,1995,176(2):273-282
- [36]Maliszewska-Kordybach B,Klimkowicz-Pawlas A,Smreczak B,et al.Ecotoxic effect of phenanthrene on nitrifying bacteria in soils of different properties[J].Journal of Environmental Quality,2007,36(6):1635-1645
- [37]Dick R P.Soil enzyme activities as integrative indicators of soil health[M]//Pankhurst C E,Doube B M,Gupta V V S R(Eds.).Biological Indicators of Soil Health.Wallingford:CAB International,1997:121-156
- [38]姚超英.痕量有机污染物DDT和PCBs复合共存时对农田土壤氧化还原酶活性的影响[J].农业环境科学学报,2008,27(3):909-913Yao C Y.Influence of coexisting trace organic pollutants,DDT and PCBs on the activity of oxidation reduction enzyme in farmland soil[J].Journal of Agro-Environment Science,2008,27(3):909-913(in Chinese)
- [39]逄焕成,严慧峻,闵九康.土壤有机氯污染的生物修复和土壤酶活性的关系[J].土壤肥料,2002(1):30-33Pang H C,Yan H J,Min J K.Study on bioremediation of organochlorinic contaminated soil and its relation to soil enzyme activity[J].Soils and Fertilizers,2002(1):30-33(in Chinese)
- [40]鄂有幸,王覃,李蕾,等.多氯联苯类化合物分子结构的理论研究[J].北京化工大学学报,2006,33(3):81-84E Y X,Wang T,Li L,et al.A theoretical study of the molecular structure of polychlorinated biphenyls[J].Journal of Beiiing University of Chemical Technology,2006,33(3):81-84(in Chinese)
- [41]滕应,郑茂坤,骆永明,等.长江三角洲典型地区农田土壤多氯联苯空间分布特征[J].环境科学,2008,29(12):3477-3482Teng Y,Zheng M K,Luo Y M,et al.Spatial distribution of soil PCBs congeners in typical area of Yangtze River Delta Region[J].Environmental Science,2008,29(12):3477-3482(in Chinese)
- [42]江萍,赵平,万洪富,等.珠江三角洲典型地区表层农田土壤中多氯联苯残留状况[J].土壤,2011,43(6):948-953Jiang P,Zhao P,Wan H F,et al.Concentrations of PCBs in agricultural soils of typical regions in Pearl River Delta[J].Soils,2011,43(6):948-953(in Chinese)
- [43]Weissenfels W D,Klewer H J,Langhoff J.Adsorption of polycyclic aromatic hydrocarbons(PAHs)by soil particles:Influence on biodegradability and biotoxicity[J].Applied Microbiology and Biotechnology,1992,36(5):689-696
- [44]Dang C K,Chauvet E,Gessner M O.Magnitude and variability of process rates in fungal diversity-litter decomposition relationships[J].Ecology Letters,2005,8(11):1129-1137
- [45]李晶,刘玉荣,贺纪正,等.土壤微生物对环境胁迫的响应机制[J].环境科学学报,2013,33(4):959-967Li J,Liu Y R,He J Z,et al.Insights into the responses of soil microbial community to the environmental disturbances[J].Acta Scientiae Circumstantiae,2013,33(4):959-967(in Chinese)
- [46]Kim J,Rhee G Y.Population dynamics of polychlorinated biphenyl-dechlorinating microorganisms in contaminated sediments[J].Applied and Environmental Microbiology,1997,63(5):1771-1776
- [47]Tiedje J M,Quensen J F,Chee-Sanford J,et al.Microbial reductive dechlorination of PCBs[J].Bioremediation,1993,4(4):231-240