张燕芬;王大勇;

• 1:东南大学发育与疾病相关基因教育部重点实验室

摘要(Abstract)：

模式动物秀丽线虫(Caenorhabditis elegans)在环境科学与生态毒理学研究中发挥着越来越重要的作用,目前利用秀丽线虫已初步建立起较为完善的环境毒物毒性评估体系.该评估体系具体由如下一些指标构成:致死率、最长寿命与半数致死天数、细胞凋亡、个体发育、生殖、运动行为、乙酰胆碱酯酶活性、学习行为、记忆行为、转基因品系、突变体、基因表达模式.论文系统总结了近十多年来利用秀丽线虫进行环境毒物毒性评估的研究体系,并对各个指标的研究方法、研究现状、存在的问题及应用中需要注意的地方进行了讨论。

关键词(KeyWords)： 评估体系;评价指标;环境毒物;秀丽线虫

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(No.30771113);; 教育部新世纪优秀人才资助项目

作者(Author): 张燕芬;王大勇;

Email:

DOI:

参考文献(References)：

• Anderson G L,Boyd W A,Williams P L.2001.Assessment ofsublethal endpoints for toxicity testing with the nematodeCaenorhabditis elegans[J].Environmental Toxicology andChemistry,20:833-838
• Anderson G L,Cole R D,Williams P L.2003.Assessingbehavioral toxicity with Caenorhabditis elegans[J].EnvironmentalToxicology and Chemistry,23:1235-1240
• Barsyte D,Lovejoy D A,Lithgow G J.2001.Longevity andheavy metal resistance in daf-2and age-1long-lived mutants ofCaenorhabditis elegans[J].The FASEB Journal,15:627-634
• Boyd W A,Cole R D,Anderson G L,Williams P L.2003.The effects of metals and food availability on the behavior ofCaenorhabditis elegans[J].Environmental Toxicology and Chemistry,22:3049-3055
• Boyd W A,Williams P L.2003.Availability of metals to thenematode Caenorhabditis elegans:Toxicity based on totalconcentrations in soil and extracted fractions[J].EnvironmentalToxicology and Chemistry,22:1100-1106
• Cai K Z,Liu X L,Guo Q Z,Xu Y J,Chen H,Yu Z L.2007.Effects of exposure to electrically generated negative airions(ENIs)on the nematode Caenorhabditis elegans[J].AsianJournal of Ecotoxicology,2:396-401(in Chinese)
• Cheng Q,Valmas N,Reilly P E B,Collins P J,Kopittke R,Ebert P R.2003.Caenorhabditis elegans mutants resistant tophosphine toxicity show increased longevity and cross-resistanceto the synergistic action of oxygen[J].Toxicological Sciences,73:60-65
• Chu K W,Chan S K W,Chow K L.2005.Improvement ofheavy metal stress and toxicity assays by coupling a transgenicreporter in a mutant nematode strain[J].Aquatic Toxicology,74:320-332
• Chu K W,Chow K L.2002.Synergistic toxicity of multipleheavy metals is revealed by a biological assay using a nematodeand its transgenic derivative[J].Aquatic Toxicology,61:53-64
• Cole R D,Anderson G L,Williams P L.2004.The nematodeCaenorhabditis elegans as a model of organophosphate-inducedmammalian neurotoxicity[J].Toxicology and Applied Pharmacology,194:248-256
• Cui Y X,McBride S J,Boyd W A,Alper S,Freedman J H.2007.Toxicogenomic analysis of Caenorhabditis elegans revealsnovel genes and pathways involved in the resistance to cadmiumtoxicity[J].Genome Biology,8:R122
• Daniells C,Duce I,Thomas D,Sewell P,Tattersall J,dePomerai D.1998.Transgenic nematodes as biomonitors ofmicrowave-induced stress[J].Mutation Research/Fundamental andMolecular Mechanisms of Mutagenesis,399:55-64
• Deng Y B,Yang Y R.2007.Use of Caenorhabditis elegans asa pollution biosensor in Xiamen seawater[J].Acta ScientiaeCircumstantiae,27:1034-1037(in Chinese)
• Dengg M,Van Meel J C A.2004.Caenorhabditis elegans asmodel system for rapid toxicity assessment of pharmaceuticalcompounds[J].Journal of Pharmacological and ToxicologicalMethods,50:209-214
• Dennis J L,Mutwakil M H A Z,Lowe K C,de Pomerai D I.1997.Effects of metal ions in combination with a non-ionicsurfactant on stress responses in a transgenic nematode[J].Aquatic Toxicology,40:37-50
• Dhawan R,Dusenbery D B,Williams P L.2000.A comparisonof metal-induced lethality and behavioral responses in thenematode Caenorhabditis elegans[J].Environmental Toxicologyand Chemistry,19:3061-3067
• Dong J,Boyd W A,Freedman J H.2008.Molecularcharacterization of two homologs of the Caenorhabditis eleganscadmium-responsive gene cdr-1:cdr-4and cdr-6[J].Journal ofMolecular Biology,376:621-633
• Donkin S G,Dusenbery D B.1993.A soil toxicity test using the nematode Caenorhabditis elegans and an effective method ofrecovery[J].Archives of Environmental Contamination andToxicology,25:145-151
• Donkin S G,Williams P L.1995.Influence of developmentalstage,salts and food presence on various end points usingCaenorhabditis elegans for aquatic toxicity testing[J].EnvironmentalToxicology and Chemistry,14:2139-2147
• Gerhardt A,Schmidt S,H!ss S.2002.Measurement ofmovement patterns of Caenorhabditis elegans(Nematoda)withthe Multispecies Freshwater Biomonitor R(MFB):a potential newmethod to study a behavioral toxicity parameter of nematodes insediments[J].Environmental Pollution,120:513-516
• Graves A L,Boyd W A,Williams P L.2005.Using transgenicCaenorhabditis elegans in soil toxicity testing[J].Archives ofEnvironmental Contamination and Toxicology,48:490-494
• Guven K,Duce J A,de Pomerai D I.1994.Evaluation of astress-inducible transgenic nematode strain for rapid aquatictoxicity testing[J].Aquatic Toxicology,29:119-137
• Harada H,Kurauchi M,Hayashi R,Eki T.2007.Shortenedlifespan of nematode Caenorhabditis elegans after prolongedexposure to heavy metals and detergents[J].Ecotoxicology andEnvironmental Safety,66:378-383
• Hitchcock D R,Black M C,Williams P L.1997.Investigationsinto using the nematode Caenorhabditis elegans for municipal andindustrial wastewater toxicity testing[J].Archives of EnvironmentalContamination and Toxicology,33:252-260
• Hughes S,Stürzenbaum S R.2007.Single and double metallothioneinknockout in the nematode C.elegans reveals cadmium dependentand independent toxic effects on life history traits[J].EnvironmentalPollution,145:395-400
• Kim J Y,Sharma R P.2004.Calcium-mediated activation of c-JunNH2-terminal kinase(JNK)and apoptosis in response to cadmiumin murine macrophages[J].Toxicological Sciences,81:518-527
• Mizuno T,Hisamoto N,Terada T,Kondo T,Adachi M,NishidaE,Kim D H,Ausubel F M,Matsumoto K.2004.TheCaenorhabditis elegans MAPK phosphatase VHP-1mediates anovel JNK-like signaling pathway in stress response[J].TheEMBO Journal,23:2226-2234
• Mutwakil M H A Z,Reader J P,Holdich D M,Smithurst P R,Candido E P M,Jones D,Stringham E G,de Pomerai D I.1997.Use of stress-inducible transgenic nematodes as biomarkersof heavy metal pollution in water samples from an English riversystem[J].Archives of Environmental Contamination andToxicology,32:146-153
• Peredney C L,Williams P L.2000.Utility of Caenorhabditiselegans for assessing heavy metal contamination in artificial soil J].Archives of Environmental Contamination and Toxicology,39:113-118
• Power R S,de Pomerai D I.1999.Effect of single and pairedmetal inputs in soil on a stress-inducible transgenic nematode[J].Archives of Environmental Contamination and Toxicology,37:503-511
• Roh J Y,Choi J.2007.Ecotoxicological evaluation of chlorpyrifos exposure on the nematode Caenorhabditis elegans[J].Ecotoxicologyand Environmental Safety,doi:10.1016/j.ecoenv.2007.11.007
• Roh J Y,Lee J,Choi J.2006.Assessment of stress-related geneexpression in the heavy metal-exposed nematode Caenorhabditiselegans:A potential biomarker for metal-induced toxicity monitoringand environmental risk assessment[J].Environmental Toxicologyand Chemistry,25:2946-2956
• Sakashita T,Hamada N,Ikeda D D,Suzuki M,Yanase S,IshiiN,Kobayashi Y.2008.Locomotion-learning behavior relationshipin Caenorhabditis elegans following gamma-ray irradiation[J].Journal of Radiation Research(Tokyo),49:285-291
• SochováI,Hofman J,Holoubek I.2007.Effects of sevenorganic pollutants on soil nematode Caenorhabditis elegans[J].Environment International,33:798-804
• Stringham E G,Candido E P M.1994.Transgenic hsp16-lacZstains of the soil nematode Caenorhabditis elegans as biologicalmonitors of environmental stress[J].Environmental Toxicologyand Chemistry,13:1211-1220
• Swain S C,Keusekotten K,Baumeister R,Stürzenbaum S R.2004.C.elegans metallothioneins:New insights into thephenotypic effects of cadmium toxicosis[J].Journal of MolecularBiology,341:951-959
• Traunspurger W,Haitzer M,H#ss S,Beier S,Ahlf W,SteinbergC.1997.Ecotoxicological assessment of aquatic sediments withCaenorhabditis elegans(Nematode)—a method for testing liquidmedium and whole-sediment samples[J].Environmental Toxicologyand Chemistry,16:245-250
• Vatamaniuk O K,Bucher E A,Sundaram M V,Rea P A.2005.CeHMT-1,a putative phytochelatin transporter,is required forcadmium tolerance in Caenorhabditis elegans[J].The Journal ofBiological Chemistry,280:23684-23690
• Wang D Y,Hu Y O,Xu X M.2007.Comparison of multi-biological toxicities between chromium exposed Caenorhabditiselegans and their progeny[J].Asian Journal of Ecotoxicology,2:298-303(in Chinese)
• Wang D Y,Shen L L,Wang Y.2007.The phenotypic andbehavioral defects can be transferred from zinc-exposed nematodesto their progeny[J].Environmental Toxicology and Pharmacology,24:223-230
• Wang D Y,Wang Y.2008a.Nickel sulfate induces numerousdefects in Caenorhabditis elegans that can also be transferred toprogeny[J].Environmental Pollution,151:585-592
• Wang D Y,Wang Y.2008b.Phenotypic and behavioral defectscaused by barium exposure in nematode Caenorhabditis elegans[J].Archives of Environmental Contamination and Toxicology,54:447-453
• Wang D Y,Yang P.2007a.Multi-biological defects caused bylead exposure exhibit transferable properties from exposed parentsto their progeny in Caenorhabditis elegans[J].Journal ofEnvironmental Sciences,19:1367-1372
• Wang D Y,Yang P.2007b.Silver exposure causes transferable defects of phenotypes and behaviors in nematode Caenorhabditiselegans[J].Environmental Bioindicators,2:89-98
• Wang S C,Tang M L,Pei B,Xiao X,Wang J,Hang H Y,Wu L J.2008.Cadmium-induced germline apoptosis inCaenorhabditis elegans:The roles of HUS1,p53,and MAPKsignaling pathways[J].Toxicological Sciences,102:345-351
• Wang X Y,Yu H X,Wang D Y.2007a.Evaluation oftoxification in acid and alkalescent industry liquid wastes frompaper mill using C.elegans[J].Environmental Science,28:876-880(in Chinese)
• Wang X Y,Yu H X,Wang D Y,Wang X R,Zhang X X,Cheng S P.2007b.Toxicity evaluation of purified terephthalicacid waste water by using model Caenorhabditis elegans[J].Chinese Journal of Applied Ecology,18:1669-1672(in Chinese)
• Wang Y,Wang D Y.2007.Copper exposure causes transferabledefects of phenotypes and behaviors in C.elegans[J].Journal ofSafety and Environment,7:10-14(in Chinese)
• Wang Y,Xie W,Wang D Y.2007.Transferable properties of multi-biological toxicity caused by cobalt exposure in Caenorhabditiselegans[J].Environmental Toxicology and Chemistry,26:2405-2412
• Williams P L,Dusenbery D B.1990.Aquatic toxicity testingusing the nematode Caenorhabditis elegans[J].EnvironmentalToxicology and Chemistry,9:1285-1290
• Yanase S,Hartman P S,Ito A,Ishii N.1999.Oxidative stresspretreatment increases the X-radiation resistance of the nematodeCaenorhabditis elegans[J].Mutation Research,426:31-39
• Ye H Y,Ye B P,Wang D Y.2008a.Trace administration ofvitamin E can retrieve and prevent3UV-irradiation-and metalexposure-induced memory deficits in nematode Caenorhabditiselegans[J].Neurobiology of Learning and Memory,90:10-18
• Ye H Y,Ye B P,Wang D Y.2008b.Evaluation of the long-termmemory for thermosensation regulated by neuronal calcium sensor-1in Caenorhabditis elegans[J].Neuroscience Bulletin,24:1-6
• 蔡克周,刘雪兰,郭巧芝,许永建,陈华,余增亮.2007.空气负离子暴露对秀丽隐杆线虫(Caenorhabditis elegans)的生物学效应[J].生态毒理学报,2:396-401
• 邓雅斌,杨玉荣.2007.利用秀丽小杆线虫Caenorhabditis elegans监测厦门岛海水水质[J].环境科学学报,27:1034-1037
• 汪洋,王大勇.2007.铜中毒引起的秀丽线虫世代间可传递的缺陷[J].安全与环境学报,7:10-14
• 王大勇,胡亚欧,许雪梅.2007.铬暴露导致的秀丽线虫多重毒性的世代间比较[J].生态毒理学报,2:298-303
• 王晓祎,于红霞,王大勇.2007a.利用模式动物线虫追踪造纸厂废水中酸碱类毒物的研究[J].环境科学,28:876-880
• 王晓祎,于红霞,王大勇,王晓蓉,张徐祥,程树培.2007b.利用模式生物线虫评价精对苯二甲酸废水的毒性[J].应用生态学报,18:1669-1672