刘晓晖;李双月;刘琦;朴丰源;邵静;李亚晨;薛鹏;

• 1:大连医科大学公共卫生学院

摘要(Abstract)：

三邻甲苯磷酸酯(tri-o-cresyl phosphate,TOCP)是一种有机磷酸酯类化合物,具神经毒性作用。研究表明星形胶质细胞是有机磷化合物(organophosphorus compounds,OPs)神经毒性作用的靶点之一。为了探讨TOCP对星形胶质细胞的毒性作用,采用大鼠C6星形胶质细胞分别经0.1、0.3、1.0和3.0 mmol·L-1TOCP染毒处理24 h,应用MTT比色法和乳酸脱氢酶(LDH)活力分析法检测细胞活力,在电子相差显微镜下观察细胞形态,二硫代二硝基苯甲酸(DNTB)比色法测定谷胱甘肽(GSH)含量和谷胱甘肽过氧化物酶(GSH-Px)活性,流式细胞仪检测分析细胞周期。结果显示,经TOCP处理24 h后,大鼠C6星形胶质细胞存活率降低,LDH释放增加,细胞形态也发生了明显的变化。GSH含量和GSH-Px活性降低,G1期细胞数量也逐渐增加。上述结果表明,TOCP对星形胶质细胞具有毒性作用,引起氧化损伤和细胞周期阻滞。

关键词(KeyWords)： 三邻甲苯磷酸酯;大鼠;星形胶质细胞;细胞毒性;氧化应激;细胞周期阻滞

Abstract:

Keywords:

基金项目(Foundation): 辽宁省自然科学基金(2013023054);; 大连医科大学本科教学改革研究项目(DYLX13036)

作者(Author): 刘晓晖;李双月;刘琦;朴丰源;邵静;李亚晨;薛鹏;

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DOI:

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