常青;郑宇铎;高娜娜;陈莉;丁书茂;

• 1:华中师范大学生命科学学院遗传调控与整合生物学湖北省重点实验室

摘要(Abstract)：

为了初步探讨邻苯二甲酸二乙基己酯(Di-(2-ethylhexyl)phthalate,DEHP)对植物的毒害作用,采用不同浓度的DEHP溶液对蚕豆根部进行处理(终含量0、0.2、2、20、200mg·kg-(1细沙)),检测根尖微核率和对幼苗茎、叶SOD活性的影响.结果表明:对蚕豆根尖进行短期(6h)处理后,各DEHP处理组微核率随DEHP含量的升高呈显著上升趋势(与对照比较,p<0.05或p<0.01).处理7d后,随DEHP含量的升高(0～20mg·kg-(1细沙)),蚕豆幼苗茎、叶SOD活性均呈逐渐升高趋势;DEHP含量在20～200mg·kg-(1细沙)时,SOD活性均略有下降.以上结果表明DEHP对蚕豆种子具有遗传毒性,且能够对蚕豆幼苗产生氧化损伤.

关键词(KeyWords)： 邻苯二甲酸二乙基己酯;蚕豆根尖细胞;微核率;超氧化物歧化酶

Abstract:

Keywords:

基金项目(Foundation): 国家科技支持计划项目(No.2006BAJ02A10)

作者(Author): 常青;郑宇铎;高娜娜;陈莉;丁书茂;

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DOI:

参考文献(References)：

• Chen L,Li X B,Yang G T,Deng L F,Ding S M.2008.DNA damage in brain cells of gold crucian carp induced by di-(2-ethylhexyl)phthalate(DEHP)[J].Asian Journal of Ecotoxicology,3:144-148(in Chinese)
• Chen Q,Sun H W,Wang B,Hu G C.2004.Effects of di(2-ethylhexyl)phthalate(DEHP)on microorganisms and animals insoil[J].Journal of Agro-Environment Science,23:1156-1159(in Chinese)
• Chen Z L,Mo D L,Qiu R L.2001.Biological damage of soilcadmium(Cd)pollution and its control[J].EnvironmentalProtection Science,27:37-39(in Chinese)
• Cong H F,Wang L X,Shao L.2008.Determination of phthalatesin vegetables by liquid chromatography-electrospray ionizationmass spectrometry[J].Food Science and Technology,(8):222-224(in Chinese)
• Fei W,Chen H Y,Cao Z,Liu Y.2005.Effects of salinitystress on physiological characteristics of tomato seeding[J].Journal of Shanghai Jiaotong University(Agricultural Science),23:5-9(in Chinese)
• Helm D.2007.Correlation between production amounts of DEHPand daily intake[J].Science of the Total Environment,388:389-391
• Hu C L,Zhong L F.2007.Genotoxic effects of di(2-ethylhexyl)phthalate in humans and animals[J].Journal ofDalian Medical University,29:185-190(in Chinese)
• Kou L J,Zhang Y Q,Li L X,Kong W C,Xie H,Wang ML.2007.Matrix solid-phase dispersion for the HPLC determinationof phthalic acid esters invegetables[J].Food and FermentationIndustries,33:120-122(in Chinese)
• Ministry of Environmental Protection of the People’s Republic ofChina.1986.Technical Specifications for Environmental Monitoring[Z].Beijing:Ministry of Environmental Protection of the People’sRepublic of China
• Ministry of Environmental Protection of the People’s Republic ofChina.2002.Analytical Method of the Water and Wastewater(4th Edition)[M].Beijing:China Environmental Science Press.769-772(in Chinese)
• Pang J M,Duan Y L,Chi B L,Fan X W,Du R P,Wang LJ.1995.Residue and toxicity of DEHP in the soil and Chinesecabbage[J].Environmental Chemistry,14:239-242(in Chinese)
• Xu G,Li F S,Wang Q H.2008.Occurrence and degradationcharacteristics of dibutyl phthalate(DBP)and di-(2-ethylhexyl)phthalate(DEHP)in typical agricultural soils of China[J].Science of the Total Environment,393:333-340
• Yin R,Lin X G,Wang S G,Zhang H Y,Chen R R,Wang JH.2004a.Influence of DBP/DEHP pollution in soil on vegetablequality[J].Journal of Agro-Environmental Science,23:1-5(inChinese)
• Yin R,Zhang H Y,Wang S G,Chen R R,Lin X G.2004b.The dynamics of di-(2-ethylhexyl)phthalate in tomato-rhizosphericsoil[J].Acta Scientiae Circumstantiae,24:444-449(in Chinese)
• Zeng Q Y,Mo C H,Cai Q Y,Wu Q T.2007.Accumulationfeatures of d(i2-ethylhexy)phthalate in various genotypes of Brassicaparachinensis-soil systems[J].Journal of Agro-Environment Science,26:2239-2244(in Chinese)
• Zhang Z L,Qu W J.2003.The Experimental Guide for PlantPhysiology[M].Beijing:Higher Education Press.268-269(inChinese)
• 陈莉,李学彬,杨光涛,邓灵福,丁书茂.2008.邻苯二甲酸二乙基己酯(DEHP)对金鲫鱼脑细胞DNA的损伤[J].生态毒理学报,3:144-148
• 陈强,孙红文,王兵,胡国臣.2004.邻苯二甲酸二异辛酯(DEHP)对土壤中微生物和动物的影响[J].农业环境科学学报,23:1156-1159
• 陈志良,莫大伦,仇荣亮.2001.镉污染对生物有机体的危害及防治对策[J].环境保护科学,27:37-39
• 丛惠芳,王丽霞,邵蕾.2008.超声波萃取-液相色谱-质谱法测定蔬菜中的酞酸酯[J].食品科技,(8):222-224
• 费伟,陈火英,曹忠,刘杨.2005.盐胁迫对番茄幼苗生理特性的影响[J].上海交通大学学报(农业科学版),23:5-9
• 国家环境保护总局.1986.环境监测技术规范[Z].北京:国家环境保护总局
• 国家环境保护总局.2002.水和废水监测分析方法(第4版)[M].北京:中国环境科学出版社.769-772
• 胡存丽,仲来福.2007.邻苯二甲酸(2-乙基己基)酯遗传毒性研究进展[J].大连医科大学学报,29:185-190
• 寇立娟,张玉倩,李兰晓,孔维超,谢惠,王明林.2007.基质固相分散-液相色谱-质谱法测定蔬菜中的邻苯二甲酸酯[J].食品与发酵工业,33:120-122
• 庞金梅,段亚利,池宝亮,樊修武,杜荣平,王丽娟.1995.DEHP在土壤和白菜中的残留及毒性分析[J].环境化学,14:239-242
• 尹睿,林先贵,王曙光,张华勇,陈瑞蕊,王俊华.2004a.土壤中DBP/DEHP污染对几种蔬菜品质的影响[J].农业环境科学学报,23:1-5
• 尹睿,张华勇,王曙光,陈瑞蕊,林先贵.2004b.邻苯二甲酸二异辛酯在番茄根际土壤中的持留动态[J].环境科学学报,24:444-449
• 曾巧云,莫测辉,蔡全英,吴启堂.2007.不同基因型菜心-土壤系统中邻苯二甲酸二(2-乙基己基)酯的分布特征研究[J].农业环境科学学报,26:2239-2244
• 张志良,瞿伟菁.2003.植物生理学实验指导[M].北京:高等教育出版社,268-269