陈洁;张积仁;

• 1:南方医科大学珠江医院肿瘤中心
• 2:广东省靶向肿瘤干预与防控研究院

摘要(Abstract)：

大量流行病学研究和体内、体外检测分析表明,长期低剂量接触农药可以导致人体细胞和分子损伤,诱导细胞凋亡。农药致细胞及DNA损伤的机制主要与DNA加合物的形成、DNA单链和/或双链的断裂有关。此外,氧化应激参与农药致细胞及DNA损伤的过程,可能成为农药致细胞及DNA损伤的促发因素。从总体上看,其具体分子机制还不十分清楚,有待进一步研究。

关键词(KeyWords)： 农药;细胞毒性;基因毒性;氧化应激

Abstract:

Keywords:

基金项目(Foundation):

作者(Author): 陈洁;张积仁;

Email:

DOI:

参考文献(References)：

• [1]Ecobichon D J.Pesticide use in developing countries[J].Toxicology,2001,160(1-3):27-33
• [2]Choi S.Critical review on the carcinogenic potential of pesticides used in Korea[J].Asian Pacific Journal of Cancer Prevention:APJCP,2014,15(15):5999-6003
• [3]Huang J,Hu R,Qiao F,et al.Impact of insect-resistant GM rice on pesticide use and farmers'health in China[J].Science China Life Sciences,2015,58(5):466-471
• [4]Pingali P L,Marquez C,Pallis F G.Pesticides and Philippine rice farmer health:A medical and economic analysis[J].American Journal of Agricultural Economics,1994,76(3):587-592
• [5]Dabrowski J M,Shadung J M,Wepener V.Prioritizing agricultural pesticides used in South Africa based on their environmental mobility and potential human health effects[J].Environment International,2014,62:31-40
• [6]Belle R,Le Bouffant R,Morales J,et al.Sea urchin embryo,DNA-damaged cell cycle checkpoint and the mechanisms initiating cancer development[J].Journal de la Societe de Biologie,2007,201(3):317-327
• [7]汤艳,张青碧,甘仲霖,等.杀虫剂诱导人外周血淋巴细胞DNA损伤[J].现代预防医学,2006,33(8):1342-1343Tang Y,Zhang Q B,Gan Z L,et al.Pesticides induced DNA damage to human peripheral lymphocytes in vitro[J].Modern Preventive Medicine,2006,33(8):1342-1343(in Chinese)
• [8]Undeger U,Basaran N.Effects of pesticides on human peripheral lymphocytes in vitro:Induction of DNA damage[J].Archives of Toxicology,2005,79(3):169-176
• [9]Nakadai A,Li Q,Kawada T.Chlorpyrifos induces apoptosis in human monocyte cell line U937[J].Toxicology,2006,224(3):202-209
• [10]Ramos-Chavez L A,Sordo M,Calderon-Aranda E,et al.A permethrin/allethrin mixture induces genotoxicity and cytotoxicity in human peripheral blood lymphocytes[J].Journal of Toxicology and Environmental Health Part A,2015,78(1):7-14
• [11]Hreljac I,Zajc I,Lah T,et al.Effects of model organophosphorous pesticides on DNA damage and proliferation of Hep G2 cells[J].Environmental and Molecular Mutagenesis,2008,49(5):360-367
• [12]Bumroongkit K,Rannala B,Traisathit P,et al.TP53 gene mutations of lung cancer patients in upper northern Thailand and environmental risk factors[J].Cancer Genetics and Cytogenetics,2008,185(1):20-27
• [13]Spiewak R.Pesticides as a cause of occupational skin diseases in farmers[J].Annals of Agricultural and Environmental Medicine:AAEM,2001,8(1):1-5
• [14]Kapka-Skrzypczak L,Cyranka M,Skrzypczak M,et al.Biomonitoring and biomarkers of organophosphate pesticides exposure-State of the art[J].Annals of Agricultural and Environmental Medicine:AAEM,2011,18(2):294-303
• [15]Collins A R,Oscoz A A,Brunborg G,et al.The comet assay:Topical issues[J].Mutagenesis,2008,23(3):143-151
• [16]许杭杰,李婧,张全,等.农药与DNA相互作用的光谱法分析[J].湖北农业科学,2014,53(1):5-8Xu H J,Li J,Zhang Q,et al.Spectroscopic analyses on the interaction of pesticides and DNA[J].Hubei Agricultural Sciences,2014,53(1):5-8(in Chinese)
• [17]卢国良,夏昭林.DNA加合物检测方法研究进展[J].职业卫生与应急救援,2005,23(1):18-20Lu G L,Xia Z L.Advance in detecting methods of DNAadduct[J].Occupational Health and Emergency Rescue,2005,23(1):18-20(in Chinese)
• [18]Prins J M,Chao C K,Jacobson S M,et al.Oxidative stress resulting from exposure of a human salivary gland cells to paraoxon:An in vitro model for organophosphate oral exposure[J].Toxicology in Vitro:An International Journal Published in Association with BIBRA,2014,28(5):715-721
• [19]黄哲玮,孙皎,孟爱英.两种体外细胞毒性检测方法的比较研究[J].上海生物医学工程,2005,26(4):205-207Huang Z W,Sun J,Meng A Y.Study on comparison of two in vitro cytotoxicity tests[J].Shanghai Journal of Biomedical Engineering,2005,26(4):205-207(in Chinese)
• [20]邵华,师以康.紫外光谱法测定混配农药的DNA加合作用[J].中国公共卫生,2003,19(1):81-82Shao H,Shi Y K.Determination of adduct actions of mixed pesticides on DNA by ultraviolet spectral method[J].China Public Health,2003,19(1):81-82(in Chinese)
• [21]孙英,张立金,闵顺耕,等.三种氨基甲酸酯类农药化合物对DNA的潜在损伤作用[J].农业环境科学学报,2004,23(3):464-466Sun Y,Zhang L J,Min S G,et al.The potentia1 trauma of three kinds of carbamate pesticides to DNA[J].Journal of Agro-environment Science,2004,23(3):464-466(in Chinese)
• [22]刘伟,朱鲁生,王军,等.利用吸收光谱法和微核法测定3种农药对DNA损伤的作用[J].农业环境科学学报,2006,25(2):531-534Liu W,Zhu L S,Wang J,et al.Determination of the damage of three pesticides on DNA by absorption spectrophotometry and micronucleus test[J].Journal of Agro-Environment Science,2006,25(2):531-534(in Chinese)
• [23]Kashanian S,Gholivand M B,Ahmadi F,et al.Interaction of diazinon with DNA and the protective role of selenium in DNA damage[J].DNA and Cell Biology,2008,27(6):325-332
• [24]Hedli C C,Snyder R,Kinoshita F K,et al.Investigation of hepatic cytochrome P-450 enzyme induction and DNAadduct formation in male CD/1 mice following oral administration of toxaphene[J].Journal of Applied Toxicology:JAT,1998,18(3):173-178
• [25]Suman G,Naravaneni R,Jamil K.In vitro cytogenetic studies of cypermethrin on human lymphocytes[J].Indian Journal of Experimental Biology,2006,44(3):233-239
• [26]Ojha A,Gupta Y.Evaluation of genotoxic potential of commonly used organophosphate pesticides in peripheral blood lymphocytes of rats[J].Human&Experimental Toxicology,2015,34(4):390-400
• [27]Zhao F,Wang B,Zhang X,et al.Induction of DNA base damage and strand breaks in peripheral erythrocytes and the underlying mechanism in goldfish(Carassius auratus)exposed to monocrotophos[J].Fish Physiology and Biochemistry,2015,41(3):613-624
• [28]Hossain M M,Richardson J R.Mechanism of pyrethroid pesticide-induced apoptosis:Role of calpain and the ERstress pathway[J].Toxicological Sciences:An Official Journal of the Society of Toxicology,2011,122(2):512-525
• [29]郑荣梁,Lesko S A,Ts'o P O P.活性氧引起哺乳动物细胞DNA损伤[J].中国科学(B辑化学生物学农学医学地学),1988(4):378-386Zheng R L,Lesko S A,Ts'o P O P.DNA damage of mammal induced by reactive oxygen[J].Scientia Sinica,1988(4):378-386(in Chinese)
• [30]褚启龙,杨克敌,王爱国.氧化应激与细胞凋亡关系的研究进展[J].卫生研究,2003,32(3):276-279Chu Q L,Yang K D,Wang A G.Research progress on oxidative stress and apoptosis[J].Journal of Hygiene Research,2003,32(3):276-279(in Chinese)
• [31]Lu X T,Ma Y,Wang C,et al.Cytotoxicity and DNAdamage of five organophosphorus pesticides mediated by oxidative stress in PC12 cells and protection by vitamin E[J].Journal of Environmental Science and Health Part B,Pesticides,Food Contaminants,and Agricultural Wastes,2012,47(5):445-454
• [32]Lavarias S,Garcia C,Crespo R,et al.Study of biochemical biomarkers in freshwater prawn Macrobrachium borellii(Crustacea:Palaemonidae)exposed to organophosphate fenitrothion[J].Ecotoxicology and Environmental Safety,2013,96:10-16
• [33]Edwards F L,Yedjou C G,Tchounwou P B.Involvement of oxidative stress in methyl parathion and parathion-induced toxicity and genotoxicity to human liver carcinoma(Hep G(2))cells[J].Environmental Toxicology,2013,28(6):342-348
• [34]Chen H M,Lee Y H,Wang Y J.ROS-triggered signaling pathways involved in the cytotoxicity and tumor promotion effects of pentachlorophenol and tetrachlorohydroquinone[J].Chemical Research in Toxicology,2015,28(3):339-350
• [35]Jin Y,Zheng S,Pu Y,et al.Cypermethrin has the potential to induce hepatic oxidative stress,DNA damage and apoptosis in adult zebrafish(Danio rerio)[J].Chemosphere,2011,82(3):398-404
• [36]El-Gohary M,Awara W M,Nassar S,et al.Deltamethrininduced testicular apoptosis in rats:The protective effect of nitric oxide synthase inhibitor[J].Toxicology,1999,132(1):1-8
• [37]Ben Amara I,Karray A,Hakim A,et al.Dimethoate induces kidney dysfunction,disrupts membrane-bound AT-Pases and confers cytotoxicity through DNA damage.Protective effects of vitamin E and selenium[J].Biological Trace Element Research,2013,156(1-3):230-242
• [38]Hsu C,Han B,Liu M,et al.Phosphine-induced oxidative damage in rats:Attenuation by melatonin[J].Free Radical Biology&Medicine,2000,28(4):636-642
• [39]Marques A,Guilherme S,Gaivao I,et al,Progression of DNA damage induced by a glyphosate-based herbicide in fish(Anguilla anguilla)upon exposure and post-exposure periods--Insights into the mechanisms of genotoxicity and DNA repair[J].Comparative Biochemistry and Physiology Toxicology&Pharmacology:CBP,2014,166:126-133
• [40]Guilherme S,Gaivao I,Santos M A,et al.DNA damage in fish(Anguilla anguilla)exposed to a glyphosate-based herbicide--Elucidation of organ-specificity and the role of oxidative stress[J].Mutation Research,2012,743(1-2):1-9
• [41]Moore P D,Yedjou C G,Tchounwou P B.Malathion-induced oxidative stress,cytotoxicity,and genotoxicity in human liver carcinoma(Hep G2)cells[J].Environmental Toxicology,2010,25(3):221-226
• [42]Saleh A M,Vijayasarathy C,Fernandez-Cabezudo M,et al.Influence of paraoxon(POX)and parathion(PAT)on apoptosis:A possible mechanism for toxicity in low-dose exposure[J].Journal of Applied Toxicology:JAT,2003,23(1):23-29
• [43]Ahmed T,Tripathi A K,Ahmed R S,et al.Assessment of phosphamidon-induced apoptosis in human peripheral blood mononuclear cells:Protective effects of N-acetylcysteine and curcumin[J].Journal of Biochemical and Molecular Toxicology,2010,24(5):286-292
• [44]Vaithinathan S,Saradha B,Mathur P P.Methoxychlor induces apoptosis via mitochondria-and Fas L-mediated pathways in adult rat testis[J].Chemico-Biological Interactions,2010,185(2):110-118
• [45]Benford D J,Price S C,Lawrence J N,et al.Investigations of the genotoxicity and cell proliferative activity of dichlorvos in mouse forestomach[J].Toxicology,1994,92(1-3):203-215
• [46]Kolaja K L,Stevenson D E,Johnson J T,et al.Subchronic effects of dieldrin and phenobarbital on hepatic DNAsynthesis in mice and rats[J].Fundamental and Applied Toxicology:Official Journal of the Society of Toxicology,1996,29(2):219-228