6种苯系物对球等鞭金藻和新月菱形藻的生长抑制Inhibitory Effect of Six Kinds of BTEXs on Growth of the Chrysophyceae Isochrysis galbana and the Diatom Nitzschia closterium
王摆;高士博;董颖;王笑月;周遵春;
摘要(Abstract):
海洋环境中苯系物污染主要来源于海洋溢油事故以及沿海石油化工企业的废水排放。为探究苯系物对海洋微藻的毒性作用,选择球等鞭金藻和新月菱形藻作为受试生物,分别考察了苯、甲苯、乙基苯、邻-二甲苯、间-二甲苯和对-二甲苯6种苯系物对2种海洋微藻生长的影响。结果表明,在0.25~64.0 mg·L-1暴露浓度下,6种苯系物对2种微藻生长具有显著的抑制作用,随着暴露浓度的升高,抑制作用明显增强。苯、甲苯、乙基苯、邻-二甲苯、间-二甲苯、对-二甲苯对球等鞭金藻的24 h的半数效应浓度(24 h-EC50)分别为:17.07、12.88、7.58、0.55、0.36、0.27 mg·L-1;对新月菱形藻的24 h-EC50值分别为:1.03、0.68、0.46、0.40、0.42、0.38 mg·L-1。上述研究结果为确定苯系物海洋环境质量标准、保护海洋生态环境提供了基础数据。
关键词(KeyWords): 苯系物;球等鞭金藻;新月菱形藻;生长抑制;毒性作用
基金项目(Foundation): 海洋公益性行业科研专项(201205012-7);; 大连市科学技术基金(2012J21DW029)
作者(Author): 王摆;高士博;董颖;王笑月;周遵春;
Email:
DOI:
参考文献(References):
- [1]李学峰,周启星.BTEX的环境质量标准研究进展[J].生态学杂志,2011,30(2):369-375Li X F,Zhou Q X.Environmental quality standard of BTEX:A review[J].Chinese Journal of Ecology,2011,30(2):369-375(in Chinese)
- [2]白红妍,韩彬,陈军辉,等.静态顶空气相色谱-质谱联用法快速测定海水中13种苯系物[J].色谱,2012,30(5):474-479Bai H Y,Han B,Chen J H,et al.Rapid determination of benzene series in seawater by gas chromatography-mass spectrometry with static headspace extraction[J].Chinese Journal of Chromatography,2012,30(5):474-479(in Chinese)
- [3]Jiang Z B,Huang Y J,Xu X Q.Advance in the toxic effects of petroleum water accommodated fraction on marine plankton[J].Acta Ecologica Sinica,2010,30(1):8-15
- [4]李艳梅,曾文炉,余强,等.海洋溢油污染的生态与健康危害[J].生态毒理学报,2011,6(4):345-351Li Y M,Zeng W L,Yu Q,et al.Adverse effect of marine oil spills on human health and ecosystem:A Review[J].Asian Journal of Ecotoxicology,2011,6(4):345-351(in Chinese)
- [5]国家海洋局.2011年中国海洋环境状况公报[EB/OL].(2012-06-30)[2013-03-20].http://www.soa.gov.cn/zwgk/hygb/zghyhjzlgb/201211/t20121107_5528.htm
- [6]屠庆华.对我国对二甲苯产业发展的若干建议[J].化学工业,2011,29(7):18-21Tu Q H.Suggestions on China PX industry development[J].Chemical Industry,2011,29(7):18-21(in Chinese)
- [7]吴金浩,王摆,王召会,等.海水中痕量苯系物的吹扫捕集/气相色谱质谱联用测定[J].分析测试学报,2012,31(12):67-71Wu J H,Wang B,Wang Z H,et al.Determination of BTEX in seawater by purge and trap gas chromatographymass spectrometry[J].Journal of Instrumental Analysis,2012,31(12):67-71(in Chinese)
- [8]Chary N S,Fernandez-Alba A R.Determination of volatile organic compounds in drinking and environmental waters[J].Trends in Analytical Chemistry,2012,32:60-75
- [9]Cavalcante R M,de Andrade M V F,Marins R V,et al.Development of a headspace-gas chromatography(HSGC-PID-FID)method for the determination of VOCs in environmental aqueous matrices:Optimization,verification and elimination of matrix effect and VOC distribution on the Fortaleza Coast,Brazil[J].Microchemical Journal,2010,96(2):337-343
- [10]朱四喜,周唯,杨红丽,等.浙江舟山潮间带沉积物中二甲苯的检测[J].广东化工,2010,37(2):125-127Zhu S X,Zhou W,Yang L H,et al.Determination of xylene in interdial sediments in Zhoushan,Zhejiang Province[J].Guangdong Chemical Industry,2010,37(2):125-127(in Chinese)
- [11]王树虎,史向阳,马万云,等.胶束电动毛细管色谱法测定海洋沉积物中的苯,甲苯和二甲苯[J].分析测试学报,2001,18(1):77-79Wang S H,Shi X Y,Ma W Y,et al.Determination of benzene homologues in marine sediment by micellar electrokinetic capillary chromatography[J].Journal of Instrumental Analysis,2001,18(1):77-79(in Chinese)
- [12]Roose P,Dewwulf J,Brinkman U A Th.Measurement of volatile organic compounds in sediments of the Scheldt Estuary and the Southern North Sea[J].Water Research,2001,35(6):1478-1488
- [13]王明清,迟晓元,秦松,等.海洋微藻总脂含量和脂肪酸组成的测定[J].中国油脂,2008,33(11):67-70Wang Q M,Chi X Y,Qin S,et al.Total lipids and fatty acid composition of marine microalgae[J].China Oils and Fats,2008,33(11):67-70(in Chinese)
- [14]汝少国,李永祺.十种有机磷农药对扁藻的毒性[J].环境科学学报,1996,16(3):337-341Ru S G,Li Y Q.Comparison Study on the toxicity of ten organophosphorus pesticides to Platymonas sp.[J].Acta Scientiae Circumstantiae,1996,16(3):337-341(in Chinese)
- [15]Levy J L,Stauber J L,Jolley D F.Sensitivity of marine microalgae to copper:The effect of biotic factors on copper adsorption and toxicity[J].Science of the Total Environment,2007,387(1):141-154
- [16]Sundb ck K,Alsterberg C,Larson F.Effects of multiple stressors on marine shallow-water sediments:Response of microalgae and meiofauna to nutrient-toxicant exposure[J].Journal of Experimental Marine Biology and Ecology,2010,388(1):39-50
- [17]唐学玺,李永祺.4种海洋微藻对久效磷的抗性与其抗氧化能力的相关性[J].海洋与湖沼,2000,31(4):414-418Tang X X,Li Y Q.Relationship between monocrotophos resistance and antioxidization in four species of marine microalgae[J].Oceanologia Et Limnologia Sinica,2000,31(4):414-418(in Chinese)
- [18]Torres M A,Barros M P,Campos S C G,et al.Biochemical biomarkers in algae and marine pollution:A review[J].Ecotoxicology and Environmental Safety,2008,71(1):1-15
- [19]江玉,吴志宏,韩秀荣,等.多环芳烃对海洋浮游植物的生物毒性研究[J].海洋科学,2002,26(1):46-50Jiang Y,Wu Z,Han X,et al.Toxicity of polycyclic aromatic hydrocarbons(PAHs)to marine algae[J].Marine Science,2002,26(1):46-50(in Chinese)
- [20]中华人民共和国国家质量监督检验检疫总局.中国国家标准化管理委员会.GB/T21805—2008化学品藻类生长抑制试验[S].北京:中国标准出版社,2008
- [21]李崇磊,王凡,刘薇,等.甲醛及苯系物混合暴露对小鼠肺脏的氧化损伤作用[J].生态毒理学报,2012,7(1):87-92Li C L,Wang F,Liu W,et al.Oxidative damage induced by formaldehyde and benzene series inhalation on mice lung[J].Asian Journal of Ecotoxicology,2012,7(1):87-92(in Chinese)
- [22]李崇磊,王凡,刘薇,等.挥发性有机物混合暴露对小鼠脑组织的氧化损伤及学习记忆能力的影响[J].生态毒理学报,2012,7(4):367-372Li C L,Wang F,Liu W,et al.Oxidative damage in brain tissue induced by exposure to mixture of volatile organic compounds and its effects on learning and memory abilities of mice[J].Asian Journal of Ecotoxicology,2012,7(4):367-372(in Chinese)
- [23]范亚维,周启星,王媛媛,等.水体BTEX污染对大型溞和霍普水丝蚓的毒性效应及水环境安全评价[J].环境科学学报,2009,29(7):1485-1490Fan Y W,Zhou Q X,Wang Y Y,et al.Toxic effects of BTEX in water on Daphnia magna and Limnodrilus hoffmeisteri and safety assessment of the aquatic environment[J].Acta Scientiae Circumstantiae,2009,29(7):1485-1490(in Chinese)
- [24]范亚维,周启星.水体甲苯、乙苯和二甲苯对斑马鱼的毒性效应[J].生态毒理学报,2009,4(1):136-141Fan Y W,Zhou Q X.Toxic effects of toluene,ethylbenzene and xylene in waters on zebrafish Brachydanio rerio[J].Asian Journal of Ecotoxicology,2009,4(1):136-141(in Chinese)