卢春凤;杨玉;商宇;王培军;陈廷玉;王丽敏;朱秋双;张井凡;

• 1:佳木斯大学

摘要(Abstract)：

为探讨ROS介导的氧化应激在异烟肼(INH)诱导L-02细胞毒性中的作用及槲皮素的干预作用,建立体外培养INH诱导L-02细胞氧化损伤模型,实验分为对照组(A)、INH组(B)、槲皮素低剂量组(C)及槲皮素高剂量组(D)。采用生化分析法检测L-02细胞培养液中天冬氨酸氨基转移酶(AST)和丙氨酸氨基转移酶(ALT)的活性;利用荧光探针检测L-02细胞线粒体内活性氧(ROS)水平;应用比色法检测L-02细胞内丙二醛(MDA)、谷胱甘肽(GSH)的含量以及主要抗氧化物酶的活性。结果表明,与对照组相比,INH能显著增加L-02细胞培养液中AST和ALT的活性、细胞线粒体内ROS水平及细胞内MDA的含量(P<0.01),并显著减少L-02细胞内GSH的含量及超氧化物歧化酶(SOD)、谷胱甘肽过氧化物酶(GSH-Px)的活性(P<0.01)。与INH组比较,槲皮素低剂量组L-02细胞培养液中AST的活性、线粒体内ROS水平及细胞内MDA的含量明显降低(P<0.05),而细胞内SOD的活性明显增加(P<0.05);高剂量槲皮素能显著降低L-02细胞培养液中AST和ALT的活性、细胞线粒体内ROS水平及细胞内MDA的含量(P<0.01),并能显著增高L-02细胞内GSH的含量和主要抗氧化物酶的活性(P<0.01)。与槲皮素低剂量组相比,槲皮素高剂量组的保护效应更明显(P<0.05)。可见,ROS介导的氧化应激在INH诱导的L-02细胞毒性中发挥了重要作用,且槲皮素对INH诱导的L-02细胞氧化损伤具有保护作用。

关键词(KeyWords)： 槲皮素;异烟肼;L-02细胞;氧化应激

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金资助项目(81373497);; 黑龙江省自然科学基金资助项目(D201248);; 佳木斯大学科学技术研究项目(Sjz2012-09)

作者(Author): 卢春凤;杨玉;商宇;王培军;陈廷玉;王丽敏;朱秋双;张井凡;

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