溴阻燃剂对肝细胞的毒性Hepatotoxicity of Brominated Flame Retardants
那广水;孙丽娟;张雯雯;王惠国;
摘要(Abstract)：
为评价四溴联苯醚(2,2,4,4-tetrabromodipheny lether,PBDE-47)、五溴联苯醚(2,2',4,4',5-pentabromodiphenylether,PBDE-99)、九溴联苯醚(polybrominated dipheny lethers206,PBDE-206)、六溴环十二烷(hexabromocyclododecane,HBCD)、四溴双酚A(tetrabromobisphenol A,TBBPA)对人肝细胞(LO2)的体外细胞毒性。将5种溴阻燃剂与人肝细胞LO2共同培养,采用四甲基偶氮噻唑蓝比色法(MTT法)检测5种溴阻燃剂对LO2细胞的相对增殖率,并按照GB/T16886.5-2003/ISO10993-5:1999标准评价5种溴阻燃剂对LO2的细胞毒性。体外实验表明,5种溴阻燃剂对LO2细胞均具有抑制作用,其中PBDE-47、PBDE-99和HBCD呈现剂量依赖关系。
关键词(KeyWords)： 四溴联苯醚;五溴联苯醚;九溴联苯醚;六溴环十二烷;四溴双酚A;LO_2细胞
基金项目(Foundation): 海洋公益性科研专项(201105013)
作者(Author): 那广水;孙丽娟;张雯雯;王惠国;
Email:
DOI:
参考文献(References)：
- [1]黄家乐,朱军山,洪在地,等.电子电气设备中塑料阻燃PBDE使用的危害性[J].家电科技,2005,7:41-43
- [2]de Wit CA.An overview of brominated flame retard-ants in the environment[J].Chemosphere,2002,46(5):583-624
- [3]Darnerud P O,Wong J,Bergman A,et al.Common vi-ral infection affects pentabrominated diphenyl ether(PBDE)distribution and metabolic and hormonal activi-ties in mice[J].Toxicology,2005,210(2/3):159-167
- [4]Branchi F,Capone A,Vitalone F,et al.Early develop-mental exposure to BDE99or Aroclor1254affects neurobehavioral profile:Interference from the administ ration route[J].Neurotoxicology,2005,26(2):183-192
- [5]Roosens L,Abdallah M A,Harrad.Exposure to hexabro-mocylododecanes(HBCDs)via dust ingestion,but not diet,correlates with concentrations in human serum:Pre-liminary results[J].Environmental Health Perspective,2009,117(11):1707-1712
- [6]Szymanska J A,Piotrowski J K.Hepatotoxicity of tet-rabromobisphenol a in rats[J].Toxicology Letters,1998,95(Supplement1):163
- [7]Fukuda N,Ito Y,Yamaguchi M,et al.Unexpected nephrotoxicity induced by tetrabromobisphenol A in newborn rats[J].Toxicology Letters,2004,150(2):145-155
- [8]吕晓迎,Kappert H F.牙科材料细胞毒性评定的新方法[J].中华口腔医学杂志,1995,30(6):377-379
- [9]Wenning R J.Uncertainties and data needs in risk as-sessment of three commercial polybrominated diphenyl ethers:Probabilistic exposure analysis and comparison with European Commission results[J].Chemosphere,2002,46(5):779-796