孙凯峰;刘莉莉;孙东;刘伟杰;何宁;段舜山;

• 1:暨南大学水生生物研究中心
• 2:环境保护部华南环境科学研究所
• 3:安徽大学江淮学院

摘要(Abstract)：

分离筛选11种微藻开展微藻对NP暴露的响应特征和去除能效研究。NP对叶绿素a含量的96 h半抑制效应浓度(EC50)介于0.60~3.33 mg·L~(-1)之间,EC50由小到大依次为:短棘盘星藻<羊角月牙藻<小球藻<衣藻<斜生栅藻<肥壮蹄形藻<卷曲纤维藻<二尾栅藻<惠氏微囊藻<四尾栅藻<蛋白核小球藻。除斜生栅藻、羊角月牙藻、肥壮蹄形藻、卷曲纤维藻之外,微藻的生长速率与EC50呈显著的正相关关系。EC50与细胞粒径、表面积以及体积总体上呈负相关关系,与微藻对NP的最大去除速率和半饱和常数呈正相关关系。NP的藻类去除过程符合一级反应动力学方程,且NP的半衰期与微藻起始总表面积呈显著的负相关关系。NP对微藻群落的影响与其浓度相关,其选择性干扰效应主要与微藻耐受性相关。NP耐受性高的种类多表现出高生长速率和去除能力。

关键词(KeyWords)： 壬基酚;微藻;急性毒性;生物去除

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金项目(21307140,41476099);; 中央级公益性科研院所基本科研业务专项(PM-zx703-201602-043,PM-zx021-201211-123)

作者(Author): 孙凯峰;刘莉莉;孙东;刘伟杰;何宁;段舜山;

Email:

参考文献(References)：

• [1]Kruger T,Long M,Bonefeld-Jorgensen C B.Plastic components affect the activation of the aryl hydrocarbon and the androgen receptor[J].Toxicology,2008,246:112-123
• [2]Bergman,Heindel J J,Kasten T,et al.The impact of endocrine disruption:A consensus statement on the state of the science[J].Environmental Health Perspectives,2013,121(4):104-106
• [3]Vandenberg L N,Colborn T,Hayes T B,et al.Regulatory decisions on endocrine disrupting chemicals should be based on the principles of endocrinology[J].Reproductive Toxicology,2013,38:1-15
• [4]Ying G G,Williams B,Kookana R.Environmental fate of alkylphenols and alkylphenol ethoxylates-A review[J].Environment International,2002,28:215-226
• [5]Sánehez-Avila J,Bonet J,Velasco G,et al.Determination and occurrence of phthalates,alkylphenols,bisphenol A,PBDEs,PCBs and PAHs in an industrial sewage grid discharging to a municipal wastewater treatment plant[J].Science of The Total Environment,2009,407(13):4157-4167
• [6]Li D H,Kim M,Shim W J,et al.Seasonal flux of nonylphenol in Han River,Korea[J].Chemosphere,2004,56(1):1-6
• [7]Stachel B,Ehrhorn U,Heemken O,et al.Xenoestrogens in the River Elbe and its tributaries[J].Environmental Pollution,2003,124(3):497-507
• [8]Heemken O P,Reincke H,Stachel B,et al.The occurrence of xenoestrogens in the Elbe River and the North Sea[J].Chemosphere,2001,45:245-259
• [9]Isobe T,Nishiyama H,Nakashima A,et al.Distribution and behavior of nonylphenol,octylphenol and nonylphenol monoethoxylate in Tokyo metropolitan area:Their association with aquatic particles and sedimentary distributions[J].Environmental Science&Technology,2001,35(6):1041-1049
• [10]Blackburn M,Kirby S,Waldock M.Concentrations of alkyphenol polyethoxylates entering UK estuaries[J].Marine Pollution Bulletin,1999,38(2):109-118
• [11]Jonkers N,Laane R,De Voogt P.Fate of nonylphenol ethoxylates and their metabolites in two Dutch estuaries:Evidence of biodegradation in the field[J].Environmental Science&Technology,2003,37(2):321-327
• [12]Ferguson P,Iden C,Brownawell B.Distribution and fate of neutral alkylphenol ethoxylate metabolites in a sewage-impacted urban estuary[J].Environmental Science&Technology,2001,35(12):2428-2435
• [13]段菁春,陈兵,麦碧娴,等.洪季珠江三角洲水系烷基酚污染状况研究[J].环境科学,2004,25(3):48-52Duan J C,Chen B,Mai B X,et al.Survey of alkyphenols in aquatic environment of Zhujiang Delta[J].Environmental Science,2004,25(3):48-52(in Chinese)
• [14]李正炎,李浩东.西瓦湖中壬基酚和双酚A的污染特征[J].青岛海洋大学学报,2003,33(6):847-853Li Z Y,Li H D.Distribution characteristics of nonylphenol and bisphenol A in Shihwa Lake[J].Journal of Ocean University of Qingdao,2003,33(6):847-853(in Chinese)
• [15]Xu J,Wang P,Guo W F,et al.Seasonal and spatial distribution of nonylphenol in Lanzhou Reach of Yellow River in China[J].Chemosphere,2006,65(9):1445-1451
• [16]傅明珠.烷基酚在近海海洋和河口环境中的浓度分布与初步生态风险评价[D].青岛:中国海洋大学,2007Fu M Z.Distribution characteristics of alkylphenols in coastal and estuary environment and the ecological risk assessment[D].Qingdao:Ocean University of China,2007(in Chinese)
• [17]沈钢,张祖麟,余刚,等.夏季海河与渤海湾中壬基酚和辛基酚污染的状况[J].中国环境科学,2005,25(6):733-736Shen G,Zhang Z L,Yu G,et al.The pollution state of nonylphenol and octylphenol in Haihe River and Bohai Bay in summer[J].Chinese Environmental Science,2005,25(6):733-736(in Chinese)
• [18]Gao Q T,Tam N F Y.Growth,photosynthesis and antioxidant responses of two microalgal species,Chlorella vulgaris and Selenastrum capricornutum,to nonylphenol stress[J].Chemosphere,2011,82:346-354
• [19]Perron M C,Juneau P.Effect of endocrine disrupters on photosystem II energy fluxes of green algae and cyanobacteria[J].Environmental Research,2011,111:520-529
• [20]谢永红,苏荣国,张丽笑,等.三丁基锡对中国近海常见海洋微藻的毒性效应[J].生态环境学报,2011,20(6-7):1075-1080Xie Y H,Su R G,Zhang L X,et al.The toxic effects of chloride tributyltin on the predominant marine algae in China coastal water[J].Ecology and Environmental Sciences,2011,20(6-7):1075-1080(in Chinese)
• [21]孟范平,李卓娜,赵顺顺,等.BDE-47对4种海洋微藻抗氧化酶活性的影响[J].生态环境学报,2009,18(5):1659-1664Meng F P,Li Z N,Zhao S S,et al.Effects of BDE-47on the antioxidase activities of four species of marine microalgae[J].Ecology and Environmental Sciences,2009,18(5):1659-1664(in Chinese)
• [22]王晓艳,蒋凤华,曹为,等.六溴环十二烷和四溴双酚A对4种海洋微藻的急性毒性[J].海洋环境科学,2013,32(6):831-835Wang X Y,Jiang F H,Cao W,et al.Acute toxic effect of hexabromocyclododecane and tetrabromobisphenol Aon four marine microalgae[J].Marine Environmental Science,2013,32(6):831-835(in Chinese)
• [23]邓结平,李赟,潘鲁青.四溴双酚A对7种海洋微藻的急性毒性[J].中国海洋大学学报,2015,45(2):54-59Deng J P,Li Y,Pan L Q.The acute toxicity of TBBPAon seven marine microalgae[J].Periodical of Ocean University of China,2015,45(2):54-59(in Chinese)
• [24]赵丽,刘征涛,冯流,等.单歧藻对烷基酚类化合物的生物降解性及QSBR研究[J].环境科学研究,2005,18(1):23-27Zhao L,Liu Z T,Feng L,et al.Study on biodegradability of alkylphenols bytolypothrix and QSBR[J].Research of Environmental Sciences,2005,18(1):23-27(in Chinese)
• [25]翟洪艳,孙红文.藻类对壬基酚微生物降解的影响[J].生态环境,2007,16(3):842-845Zhai H Y,Sun H W.The effect of algae on biodegradation of nonylphenol by microorganism[J].Ecology and Environment,2007,16(3):842-845(in Chinese)
• [26]彭章娥,杨海真,汪蓓蓓,等.淡水藻类对辛基酚的吸附行为研究[J].环境科学,2009,30(12):3652-3657Peng Z E,Yang H Z,Wang B B,et al.Study on the sorption of 4-octylphenol by freshwater algae[J].Environmental Sciences,2009,30(12):3652-3657(in Chinese)
• [27]Sun K F,Liu W J,Liu L L,et al.Ecological risks assessment of organophosphorus pesticides on bloom of Microcystis wesenbergii[J].International Biodeterioration&Biodegradation,2013,77:98-105
• [28]Liu Y,Guan Y T,Gao Q T,et al.Cellular responses,biodegradation and bioaccumulation of endocrine disrupting chemicals in marine diatom Navicula incerta[J].Chemosphere,2010,80:592-599
• [29]Correa-Reyes G,Viana M T,Marquez-Rocha F J,et al.Nonylphenol algal bioaccumulation and its effect through the trophic chain[J].Chemosphere,2007,68:662-670
• [30]Babu B,Wu J T.Biodegradation of phthalate eaters by cyanobacteria[J].Journal of Phycology,2010,46:1106-1113
• [31]Gao Q T,Wong Y S,Tam N F Y.Removal and biodegradation of nonylphenol by different Chlorella species[J].Marine Pollution Bulletin,2011,63:445-451
• [32]Gao Q T,Wong Y S,Tam N F Y.Removal and biodegradation of nonylphenol by immobilized Chlorella vulgaris[J].Bioresource Technology,2011,102:10230-10238
• [33]Baptista M,Stoichev T,Basto M C P,et al.Fate and effects of octylphenol in a Microcystis aeruginosa culture medium[J].Aquatic Toxicology,2009,92:59-64
• [34]Zhou G J,Peng F Q,Yang B,et al.Cellular responses and bioremoval of nonylphenol and octylphenol in the freshwater green microalga Scenedesmus obliquus[J].Ecotoxicology and Environmental Safety,2013,87:10-16
• [35]Peng Z E,Wu F,Deng N S.Photodegradation of bisphenol A in simulated lake water containing algae,humic acid and ferric ions[J].Environmental Pollution,2006,144:840-846
• [36]Nakajima N,Teramoto T,Kasai F,et al.Glycosylation of bisphenol A by freshwater microalgae[J].Chemosphere,2007,69:934-941