吴丽华;陈燕飞;陈鹏;仪慧兰;

• 1:太原师范学院生物系
• 2:山西大学生命科学学院

摘要(Abstract)：

以模式生物酿酒酵母为材料,研究亚砷酸钠对细胞生长、抗氧化酶活性、丙二醛(MDA)含量及胞内活性氧(ROS)水平的影响。结果显示,加入亚砷酸钠(终浓度0.1~0.6 mmol·L~(-1))后,培养液在600 nm处的光密度值(OD600值)低于对照组,并呈浓度依赖性降低。经亚砷酸钠处理12 h后,酵母细胞中过氧化物酶(POD)、过氧化氢酶(CAT)、超氧化物歧化酶(SOD)和总抗氧化能力(T-AOC)活性均增高,但胞内ROS水平和MDA含量与对照组无显著差异。砷处理24 h后,POD在0.2 mmol·L~(-1)砷处理组中活性最高,而CAT、SOD和T-AOC活性呈浓度依赖性增高;胞内ROS水平和MDA含量在高浓度砷组(0.4和0.6 mmol·L~(-1))显著增高。结果表明,亚砷酸钠可抑制酵母细胞生长,改变细胞内抗氧化酶活性,较高浓度时可引起细胞氧化损伤。

关键词(KeyWords)： 亚砷酸钠;酵母菌;抗氧化物酶;丙二醛;ROS

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金青年基金(21307087);; 山西省高等学校大学生创新创业项目(2015363)

作者(Author): 吴丽华;陈燕飞;陈鹏;仪慧兰;

Email:

DOI:

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