章强;辛琦;强丽媛;程金平;

• 1:华东师范大学河口海岸学国家重点实验室
• 2:香港城市大学深圳研究院

摘要(Abstract)：

近年来工业和养殖业中铜和四环素的滥用,导致了一定程度的水环境污染问题。为探究铜与四环素对水生生物的毒害作用,选择斑马鱼作为受试生物,研究了铜及其与四环素的联合暴露对斑马鱼胚胎的毒性效应,并进一步探索了其中可能的致毒机制。结果表明:铜在低浓度下(10%致死浓度LC10=2.5μg·L~(-1),10%效应浓度EC_(10)=0.1μg·L~(-1))明显延迟了斑马鱼胚胎的孵化、卵黄囊吸收、头部、鱼鳔和体长等生长指标的发育,同时在心脏区域引起了明显的细胞凋亡效应。幼鱼体内总铜含量检测结果显示低浓度下铜的生物利用度相对更高。基因表达结果显示环境浓度的铜可能通过影响神经和心脏相关基因的表达引起斑马鱼胚胎的神经发育和心脏发育异常。铜和四环素的联合暴露实验结果表明二者的复合污染类型为拮抗作用,且两者相互作用可以形成络合物。综合以上结论,说明环境浓度的铜可能通过细胞凋亡、分子水平的变化等方式对水生生物的早期生长发育产生危害,如延迟生长发育、神经及心脏发育异常,另外铜可通过和四环素等环境中其他污染物的结合改变铜的生物有效性和毒性。

关键词(KeyWords)： 铜;四环素;络合物;斑马鱼胚胎;神经发育;心脏发育

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41101489);; 广东省自然科学基金(s2012010010847);; 教育部新世纪优秀人才计划项目(NECT-12-0181);; 河口海岸学国家重点实验室自主课题(2012RCDW-01)

作者(Author): 章强;辛琦;强丽媛;程金平;

Email:

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