章强;辛琦;强丽媛;程金平;

• 1:华东师范大学河口海岸学国家重点实验室
• 2:香港城市大学深圳研究院

摘要(Abstract)：

近年来工业和养殖业中铜和四环素的滥用,导致了一定程度的水环境污染问题。为探究铜与四环素对水生生物的毒害作用,选择斑马鱼作为受试生物,研究了铜及其与四环素的联合暴露对斑马鱼胚胎的毒性效应,并进一步探索了其中可能的致毒机制。结果表明:铜在低浓度下(10%致死浓度LC10=2.5μg·L~(-1),10%效应浓度EC_(10)=0.1μg·L~(-1))明显延迟了斑马鱼胚胎的孵化、卵黄囊吸收、头部、鱼鳔和体长等生长指标的发育,同时在心脏区域引起了明显的细胞凋亡效应。幼鱼体内总铜含量检测结果显示低浓度下铜的生物利用度相对更高。基因表达结果显示环境浓度的铜可能通过影响神经和心脏相关基因的表达引起斑马鱼胚胎的神经发育和心脏发育异常。铜和四环素的联合暴露实验结果表明二者的复合污染类型为拮抗作用,且两者相互作用可以形成络合物。综合以上结论,说明环境浓度的铜可能通过细胞凋亡、分子水平的变化等方式对水生生物的早期生长发育产生危害,如延迟生长发育、神经及心脏发育异常,另外铜可通过和四环素等环境中其他污染物的结合改变铜的生物有效性和毒性。

关键词(KeyWords)： 铜;四环素;络合物;斑马鱼胚胎;神经发育;心脏发育

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41101489);; 广东省自然科学基金(s2012010010847);; 教育部新世纪优秀人才计划项目(NECT-12-0181);; 河口海岸学国家重点实验室自主课题(2012RCDW-01)

作者(Author): 章强;辛琦;强丽媛;程金平;

Email:

DOI:

参考文献(References)：

• [1]潘科,朱艾嘉,徐志斌,等.中国近海和河口环境铜污染的状况[J].生态毒理学报,2014,9(4):618-631Pan K,Zhu A J,Xu Z B,et al.Copper contamination in coastal and estuarine waters of China[J].Asian Journal of Ecotoxicology,2014,9(4):618-631(in Chinese)
• [2]Flemming C,Trevors J.Copper toxicity and chemistry in the environment:A review[J].Water,Air,and Soil Pollution,1989,44(1-2):143-158
• [3]Xu L,Wang T Y,Ni K,et al.Metals contamination along the watershed and estuarine areas of southern Bohai Sea,China[J].Marine Pollution Bulletin,2013,74(1):453-463
• [4]Pujin V,Djukie N,Maletin S,et al.Content of heavy metals in some fish species in the section of the Danube flowing through Voiv dina[J].Water Science&Technology,1990,22(5):79-85
• [5]Stohs S J,Bagchi D.Oxidative mechanisms in the toxicity of metal ions[J].Free Radical Biology Medicine,1995,18(2):321-336
• [6]De Boeck G,Meeu W,De Coen W,et al.Tissue-specific Cu bioaccumulation patterns and differences in sensitivity to waterborne Cu in three freshwater fish:Rainbow trout(Oncorhynchus mykiss),common carp(Cyprinus carpio)and gibel carp(Carassius auratus gibelio)[J].Aquatic Toxicology,2004,70(3):179-188
• [7]Wang W X,Yang Y,Guo X,et al.Copper and zinc contamination in oysters:Subcellular distribution and detoxification[J].Environmental Toxicology and Chemistry,2011,30(8):1767-1774
• [8]Abdel-Tawwab M,Mousa M A A,Abbass F E.Growth performance and physiological reponse of African catfish,Clarias gariepinus(B.)fed organic selenium prior to the exposure to environmental copper toxicity[J].Aquaculture,2007,272(1-4):335-345
• [9]Johnson A,Carew E,Sloman K A.The effects of copper on the morphological and functional development of zebrafish embryos[J].Aquatic Toxicology,2007,84(4):431-438
• [10]Westernhagen von H.Sublethal effects of pollutants on fish eggs and larvae[D].New York,USA:Academic Press,1988:253-234
• [11]姚纪花,周平凡.铜、锌和甲胺磷对大鳞副泥鳅胚胎发育和仔鱼成活的影响[J].上海水产大学学报,1997,6(1):11-16Yao J H,Zhou P F.Effects of copper,zinc and methamidophos on the embryo development and larva survival of loach Paramisgurns dabyanus sauage[J].Journal of Shanghai Fisheries University,1997,6(1):11-16(in Chinese)
• [12]柳敏海,陈波,罗海忠.五种重金属对早繁鮸鱼胚胎和仔鱼的毒性效应[J].海洋渔业,2007,29(1):57-62Liu M H,Chen B,Luo H Z.Toxic effects of five heavymetals on the embryos and larvae of Miichthys miiuyBasilewsky during early developing stages[J].Marine Fisheries,2007,29(1):57-62(in Chinese)
• [13]吴鼎勋,洪万树.四种重金属对鮸状黄姑鱼胚胎和仔鱼的毒性[J].台湾海峡,1999,18(2):186-190Wu D X,Hong W S.Study on toxicity of four heavy metals to embryo and larval of Nibea miichthioides[J].Journal of Oceangraphy in Taiwan Strait,1999,18(2):186-190(in Chinese)
• [14]柳学周,徐永江,兰功刚.几种重金属离子对半滑舌鳎胚胎发育和仔稚鱼的毒性效应[J].海洋水产研究,2006,27(2):33-42Liu X Z,Xu Y J,Lan G G.Toxic effects of several heavy metals on the embryos,larvae of Cynoglossus semilaevis Günther[J].Marine Fisheries Research,2006,27(2):33-42(in Chinese)
• [15]Koivisto S,Ketola M.Effects of copper on life-history traits of Daphnia pulex and Bosmina longirostris[J].Aquatic Toxicology,1995,32(2/3):255-269
• [16]Reichelt-Brushett A J,Harrison P L.The effect of selected trace metals on the fertilization success of several scleractinian coral species[J].Coral Reefs,2005,24:524-534
• [17]Garnacho E,Tyler P A,Peck L S.Reproduction,seasonality,and copper toxicity in the coastal mysid Praunus flexuosus[J].Journal of the Marine Biological Association of the United Kingdom,2001,81:433-440
• [18]Sadiq M.Toxic Metal Chemistry in Marine Environments[M].1992
• [19]王红艳,郜洪文,周丹红.氯磺酚偶氮罗丹宁分光光度法测定天然水中痕量铜(Ⅱ)[J].光谱实验室,2010,27(3):1172-1177Wang H Y,Hao H W,Zhou D H.Determination of trace amounts of copper(II)in natural water by spectrophotometry with sulfochlorophenolazorhodanine[J].Chnese Journal of spectroscopy Laboratory,2010,27(3):1172-1177(in Chinese)
• [20]沈群辉,冀秀玲,傅淑珺,等.黄浦江水域抗生素及抗性基因污染初步研究[J].生态环境学报,2012,21(10):1717-1723Shen Q H,Ji X L,Fu S J,et al.Preliminary studies on the pollution levels of antibiotic and antibiotic resistance genes in Huangpu River,China[J].Ecology and Environmental Sciences,2012,21(10):1717-1723(in Chinese)
• [21]Lunden T,Miettinen S,Lonnstroml L G,et al.Influence of oxytetracycline and oxolinic acid on the immune response of rainbow trout(Oncorhynchus mykiss)[J].Fish&Shellfish Immunology,1998,8(3):217-230
• [22]曲甍甍,孙立伟,陈鋆,等.兽药添加剂阿散酸和土霉素的毒理学研究[J].农业环境科学学报,2004,23(2):240-242Qu M M,Sun L W,Chen J,et al.Toxicological characters of arsanilic acid and oxytetracycline[J].Journal of AgroEnvironment Science,2004,23(2):240-242(in Chinese)
• [23]李兆利,陈海刚,徐韵,等.3种兽药及饲料添加剂对鱼类的毒理效应[J].生态与农村环境学报,2006,22(1):84-86Li Z L,Chen H G,Xu Y,et al.Toxicological effects of three veterinary drugs and feed additives on fish[J].Journal of Ecology and Rural Environment,2006,22(1):84-86(in Chinese)
• [24]刘臻.典型抗生素对热带爪蟾胚胎生态毒性的研究[D].上海:上海海洋大学,2012Liu Z.Ecological toxicity of selected antibiotics on the embryo of Xenopus tropicalis[D].Shanghai:Shanghai Marine University,2012(in Chinese)
• [25]Westerfield M.The Zebrafish Book[M].Eugene:University of Oregon Press,1993
• [26]Griffitt R J,Lavelle C M,Kane A S,et al.Chronic nanoparticulate silver exposure results in tissue accumulation and transcriptomic changes in zebrafish[J].Aquatic Toxicology,2013,130:192-200
• [27]Zeng C,Sun H,Xie P,et al.The role of apoptosis in MCLR-induced developmental toxicity in zebrafish embryos[J].Aquatic Toxicology,2014,149:25-32
• [28]张亚辉,刘征涛,王一喆,等.Cu2+和Cd2+对斑马鱼胚胎早期发育的联合毒性[J].环境科学研究,2010,23(11):1415-1420Zhang Y H,Liu Z T,Wang Y Z,et al.Joint toxicity of Cu2+and Cd2+to early development of zebrafish embryos[J].Research of Environmental Science,2010,23(11):1415-1420
• [29]张丽玉.重金属铜对斑马鱼的急性毒理试验[D].漳州:漳州师范学院,2004
• [30]Yuan G G,Wang Y M,Yuan X Y,et al.T-2 toxin induces developmental toxicity and apoptosis in zebrafish embryos[J].Journal of Environmental Sciences,2014,26(4):917-925
• [31]Zhang Y,Cai X Y,Lang X M,et al.Insights into aquatic toxicities of the antibiotics oxytetracycline and ciprofloxacin in the presence of metal:Complexation versus mixture[J].Environmental Pollution,2012,166:48-56
• [32]Luckenbach T,Kilian M,Triebakorn R,et al.Fish early life stage tests as a tool to assess embryo toxic potentials in small streams[J].Journal of Aquatic Ecosystem Stress Recovery,2001,8:355-370
• [33]Zhang W,Miao Y,Lin K,et al.Toxic effects of copper ion in zebrafish in the joint presence of Cd Te QDs[J].Environmental Pollution,2013,176:158-164
• [34]Yamagami K.Mechanisms of hatching in fish:Secretion of hatching enzyme and enzymatic choriolysis[J].American Zoologist,1981,21(2):459-471
• [35]Jnsson M E,Kubota A,Timme-Laragy A R,et al.Ahr2-dependence of PCB126 effects on the swim bladder in relation to expression of CYP1 and cox genes in developing zebrafish[J].Toxicology and Applied Pharmacology,2012,265(2):166-174
• [36]Luzio A,Monteiro S M,Fontaínhas-Fernandes A A,et al.Copper induced upregulation of apoptosis related genes in zebrafish(Danio rerio)gill[J].Aquatic Toxicology,2013,128-129:183-189
• [37]Tropepe V,Sive H L.Can zebrafish be used as a model to study the neurodevelopmental causes of autism?[J].Genes Brain and Behavior,2003,2:268-281
• [38]Barres B A.The mystery and magic of glia:A perspective on their roles in health and disease[J].Neuron,2008,60:430-440
• [39]Wilson S I,Edlund T.Neural induction:Toward a unifying mechanism[J].Nature Neuroscience,2001,4:1161-1168
• [40]Pashmforoush M,Lu J T,Chen H,et al.Nkx2-5 pathways and congenital heart disease;loss of ventricular myocyte lineage specification leads to progressive cardiomyopathy and complete heart block[J].Cell,2004,117:373-386
• [41]Zhang Y Y,Wang C G,Huang L X,et al.Low-level pyrene exposure causes cardiac toxicity in zebrafish(Danio rerio)embryos[J].Aquatic Toxicology,2012,114-115:119-124
• [42]Huang T C,Chuang A,Wu S J,et al.Relationships among the species and forms of copper and biomass along the Erhjin Chi coastal water[J].Acta Oceanographica Taiwanica,1990,25:65-76◆