多壁碳纳米管存在环境下Pb、Zn对斑马鱼毒性的变化The Change of the Toxicity of Pb and Zn on Zebrafish in the Case of the Existence of Multi-Walled Carbon Nanotubes
刘信勇;朱琳;
摘要(Abstract)：
研究了重金属铅、锌对斑马鱼的急性毒性效应及在人工碳纳米材料——多壁碳纳米管存在环境下,铅、锌对斑马鱼毒性的变化.结果显示,随着铅(Pb(NO3)2)、锌(ZnSO4)浓度的增加以及染毒时间的延长,斑马鱼死亡率逐渐增加;单一多壁碳纳米管悬液(10mg·L-1)对斑马鱼无明显毒性效应.Pb和Zn对斑马鱼24h、48h和96h的LC50分别为5.38mg·L-1、3.99mg·L-1、3.83mg·L-1和26.37mg·L-1、21.39mg·L-1、20.62mg·L-1;在多壁碳纳米管存在条件下,二者对斑马鱼24h、48h和96h的LC50分别为2.74mg·L-1、2.26mg·L-1、2.15mg·L-1和21.85mg·L-1、17.17mg·L-1、16.77mg·L-1.多壁碳纳米管存在条件下铅、锌对斑马鱼的LC50显著降低,提示碳纳米材料可能会增加重金属对水生生物的毒性.
关键词(KeyWords)： 壁碳纳米管;斑马鱼;铅;锌;毒性效应;LC50
基金项目(Foundation): 高等学校博士学科点专项科研基金(No.20070055033)
作者(Author): 刘信勇;朱琳;
Email:
参考文献(References)：
- Brumfiel G.2003.Nanotechnology:A little knowledge[J].Nature,424(6946):246-248
- Colvin V L.2003.The potential environmental impact ofengineered nanomaterials[J].Nature Biotechnology,21(10):1166-1170
- General Administration of Quality Supervision,Inspection andQuarantine of the People’s Republic of China,Ministry ofEnvironmental Protection of the People’s Republic of China.1991.GB/T13267-1991Water Quality-Determination of theAcute Toxicity of Substance to a Freshwater Fish(Brachydaniorerio namilton-Buchanan)[S].Beijing:China Standards Press(in Chinese)
- Gong X Z,Tang J N,Gu K M,Yang Q P.2005.Study ofdispersion of carbon nanotubes[J].Guangdong ChemicalIndustry,(4):7-9(in Chinese)
- Health and Safety Executive(UK).2004.Nanomaterials—a Riskto Health at Work[OL].2007-06-22.http://www.hsl.gov.uk
- Hyung H,Fortner J D,Hughes J B,Kim J H.2007.Naturalorganic matter stabilizes carbon nanotubes in the aqueous phase[J].Environmental Science&Technology,41(1):179-184
- Kong Z M.2006.Environmental Toxicology[M].Nanjing:Nanjing University Press(in Chinese)
- Maynard A D,Aitken R J,Butz T,Colvin V,Donaldson K,Oberdrster G,Philbert M A,Ryan J,Seaton A,Stone V,Tinkle S S,Tran L,Walker N J,Warheit D B.2006.Safehandling of nanotechnology[J].Nature,444:267-269
- Moore M N.2006.Do nanoparticles present ecotoxicologicalrisks for the health of the aquatic environment-[J].EnvironmentInternational,32(8):967-976
- Nel A,Xia T,Mdler L,Li N.2006.Toxic potential ofmaterials at the nanolevel[J].Science,311(5761):622-627
- Oberdrster E.2004.Manufactured nanomaterials(fullerenes,C60)induce oxidative stress in the brain of juvenile largemouth bass[J].Environmental Health Perspectives,112(10):1058-1062
- Roberts A P,Mount A S,Seda B,Souther J,Qiao R,Lin S,Ke P C,Rao A M,Klaine S J.2007.In vivo biomodificationof lipid-coated carbon nanotubes by Daphnia magna[J].Environmental Science&Technology,41(8):3025-3029
- Service R F.2003.Nanomaterials show signs of toxicity[J].Science,300(5617):243
- Service R F.2005.Nanotechnology.Calls rise for more researchon toxicology of nanomaterials[J].Science,310(5754):1609
- Stone V,Donaldson K.2006.Signs of stress[J].NatureNanotechnology,1(1):23-24
- Swiss Reinsurance Company(Swiss).2004.Nanotechnology-smallmatters,many unknows[OL].2007-06-22.http://www.swissre.com
- Tremblay J F.2002.Mitsubishi chemical aims at breakthrough[J].Chemical&Engineering News,80(49):16-17
- U.S.EPA.2007.U.S.Environmental Protection Agency FinalNanotechnology White Paper[OL].2007-03-09.http://www.epa.gov/osa/Pdfs/nanotech/epa-nanotechnology-white-paper-final-february-2007.pdf
- UK Government Research(UK).2006.Characterizing thepotential risks posed by engineered nanoparticles.progress report[OL].2007-06-22.http://www.defra.gov.uk
- Wang B,Feng W Y,Zhao Y L,Xing G M,Chai Z F,WangH F,Jia G.2005.Research progress on biological effect andtoxicology of nanometer materials[J].Science in China(Ser.B),35(1):1-10(in Chinese)
- Xi Z G,Lin Z Q.2006.Researching advancements andperspectives of the influences of nanoscale substances on ecologicalenvironment and biological safety[J].Asian Journal ofEcotoxicology,1(3):203-208(in Chinese)
- Zhou G X,Zhang Y T.2005.A study of the even distributionof the carbon nanotubes[J].Journal of Huaibei Industry TeachersCollege(Natural Science Edition),26(3):13-16(in Chinese)
- Zhu S,Oberdrster E,Haasch M L.2006.Toxicity of anengineered nanoparticle(fullerene,C60)in two aquatic species,Daphnia and fathead minnow[J].Marine Environmental Research,62(Supple.1):S5-S9
- Zhu X,Zhu L,Li Y,Duan Z,Chen W,Alvarez P J.2007.Developmental toxicity in zebrafish(Danio rerio)embryos afterexposure to manufactured nanomaterials:buckminsterfullereneaggregates(nC60)and fullerol[J].Environmental Toxicology andChemistry,26(5):976-979
- 龚晓钟,汤皎宁,古坤明,杨钦鹏.2005.碳纳米管分散性的研究[J].广东化工,(4):7-9
- 国家质量监督检验检疫总局,环境保护部.1991.GB/T13267-1991水质物质对淡水鱼(斑马鱼)急性毒性测定方法[S].北京:标准出版社
- 孔志明.2006.环境毒理学[M].南京:南京大学出版社
- 汪冰,丰伟悦,赵宇亮,邢更妹,柴之芳,王海芳,贾光.2005.纳米材料生物效应及其毒理学研究进展[J].中国科学(B辑),35(1):1-10
- 袭著革,林治卿.2006.纳米尺度物质对生态环境的影响及其生物安全性的研究进展与展望[J].生态毒理学报,1(3):203-208
- 周光茜,张英堂.2005.碳纳米管在水中均匀分散的研究[J].淮北煤炭师范学院学报(自然科学版),26(3):13-16