铅(Pb~(2+))致高血压大鼠胸主动脉病变的相关基因及信号转导途径The Related Genes and Possible Signal Transduction Paths in the Pathological Changes of the Thoracic Aortae of the Hypertensive Rats Induced by Exposure to Lead(Pb~(2+))
张乐丰;王生;彭双清;董延生;韩刚;
摘要(Abstract)：
采用表达芯片研究铅(Pb2+)致高血压大鼠模型病变主动脉的基因表达谱,以及可能涉及的信号转导途径.结果表明,Pb2+致高血压大鼠和对照组相比,在3463个已知基因和表达序列标签(EST)中,有24个基因和1个EST表达上调幅度≥2倍;有2个基因和1个EST表达下调幅度≥2倍.在差异表达≥2倍的26个基因中,有13个上调的基因与细胞凋亡、局部粘附、细胞生长、增殖、细胞迁移、平滑肌兴奋、胶原重构等生物学过程相关;2个下调基因与能量代谢有关.表达差异基因相对集中的信号途径是与细胞运动、增殖和存活关系密切的局部粘附信号途径.
关键词(KeyWords)： 高血压;基因芯片;铅(Pb~(2+));大鼠
基金项目(Foundation): 国家自然科学基金项目(No.39400113);; 军事医学科学院创新基金项目(No.2003A)
作者(Author): 张乐丰;王生;彭双清;董延生;韩刚;
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DOI:
参考文献(References)：
- Ashburner M,Ball C A,Blake J A,Botstein D,Butler H,Cherry J M,Davis A P,Dolinski K,Dwight S S,Eppig J T,Harris M A,Hill D P,Issel-Tarver L,Kasarskis A,Lewis S,Matese J C,Richardson J E,Ringwald M,Rubin G M,Sherlock G.2000.Gene ontology:Tool forthe unification of biology.The Gene Ontology Consortium[J].NatGenet,25:25-29
- Blaschke F,Bruemmer D,Law R E.2004.Egr-1is a major vascularpathogenic transcription factor in atherosclerosis and restenosis[J].Rev Endocr Metab Disord,5:249-254
- Courtois E,Marques M,Barrientos A,Casado S,Lopez-Farre A.2003.Lead-induced downregulation of soluble guanylate cyclase in isolatedrat aortic segments mediated by reactive oxygen species andcyclooxygenase-2[J].J Am Soc Nephrol,14:1464-1470
- Fujiwara Y,Kaji T.1999.Possible mechanism for lead inhibition ofvascular endothelial cell proliferation:A lower response to basicfibroblast growth factor through inhibition of heparan sulfate synthesis[J].Toxicology,133:147-157
- Gunthert A R,Grundker C,Hollmann K,Emons G.2002.Luteinizing hormone-releasing hormone induces JunD-DNA binding and extendscell cycle in human ovarian cancer cells[J].Biochem Biophys ResCommun,294:11-15
- Khachigian L M,Fahmy R G,Zhang G,Bobryshev Y V,Kaniaros A.2002.c-Jun regulates vascular smooth muscle cell growth andneointima formation after arterial injury.Inhibition by a novel DNAenzyme targeting c-Jun[J].J Biol Chem,277:22985-22991
- Kitamura A,Kagami S,Urushihara M,Kondo S,Yoshizumi M,TamakiT,Kuroda Y.2002.Endothelin-1is a potent stimulator of alpha1beta1integrin-mediated collagen matrix remodeling by rat mesangial cells[J].Biochem Biophys Res Commun,299:555-561
- Nawrot T S,Staessen J A.2006.Low-level environmental exposure tolead unmasked as silent killer[J].Circulation,114:1347-1349
- Song W.1997.Vessel smooth muscle[A]//Han Q D,Wen Y Y(Eds.).Vessel biology[C].Beijing:Associated Press of Beijing MedicalUniversity and Peking Union Medical College,3-23(in Chinese)
- Unoki H,Furukawa K,Yonekura H,Ueda Y,Katsuda S,Mori M,Nakagawara K,Mabuchi H,Yamamoto H.2003.Up-regulation of cyr61in vascular smooth muscle cells of spontaneously hypertensive rats[J].Lab Invest,83:973-982
- Vaziri N D,Ding Y.1999.Nitric oxide synthase expression in thecourse of lead induced hypertension[J].Hypertension,34:558-562
- 宋为.1997.血管平滑肌[A]//韩启德,文允镒(主编).血管生物学[C].北京:北京医科大学中国协和医科大学联合出版社,3-23