成团泛菌(Pantoea agglomerans)对镉胁迫下蒌蒿耐受性的影响Effects of Pantoea agglomerans on Tolerance of Artemisia selengensis under Cadmium Stress
周小梅;赵运林;董萌;库文珍;梁曾恩妮;
摘要(Abstract):
为探讨功能微生物对镉(Cd)胁迫下蒌蒿耐受性的影响,以成团泛菌(Pantoea agglomerans)J2为对象,通过盆栽试验,研究不同Cd处理水平下J2菌对蒌蒿生长、生理、Cd富集转运与根际微生态的影响。结果表明:J2菌可显著促进蒌蒿株高、地上部干重、地下部干重、总生物量及根冠比的增加。在J2菌的作用下,蒌蒿叶绿素a、叶绿素b和总叶绿素含量以及超氧化物歧化酶(SOD)、过氧化物酶(POD)和过氧化氢酶(CAT)活性均显著增加,丙二醛(MDA)含量明显减少。J2菌可显著促进蒌蒿对Cd的富集转运,蒌蒿地上部、地下部与总Cd含量均显著增加,地上部生物富集系数(BCF)明显大于地下部,转运系数(TF)相比于对照增加了7.5%~37.4%。J2菌可显著增加蒌蒿根际土壤细菌、放线菌数量及微生物总量,明显减少真菌数量。研究表明成团泛菌J2对蒌蒿生长、生理、Cd富集转运与根际微生态可产生积极影响,在一定程度上可提高蒌蒿植株对Cd的耐受性。
关键词(KeyWords): 成团泛菌;镉胁迫;蒌蒿;耐受性
基金项目(Foundation): 湖南省自然科学基金(2015JJ4012,2016JJ4015);; 湖南省教育厅优秀青年项目(15B045)
作者(Author): 周小梅;赵运林;董萌;库文珍;梁曾恩妮;
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参考文献(References):
- [1]董萌,赵运林,雷存喜,等.洞庭湖湿地Cd富集植物蒌蒿(Artemisia selengensis)的耐性生理机制研究[J].生态毒理学报,2013,8(1):111-120Dong M,Zhao Y L,Lei C X,et al.Physiological mechanism of Cd-tolerance of Artemisia selengensis grown in Dongting Lake Wetland[J].Asian Journal of Ecotoxicology,2013,8(1):111-120(in Chinese)
- [2]Zhang X X,Li C J,Nan Z B.Effects of cadmium stress on growth and anti-oxidative systems in Achnatherum inebrians symbiotic with Neotyphodium gansuense[J].Journal of Hazardous Materials,2010,175:703-709
- [3]Sheng X F,Sun L N,Huang Z,et al.Promotion of growth and Cu accumulation of bio-energy crop(Zea mays)by bacteria:Implications for energy plant biomass production and phytoremediation[J].Journal of Environmental Management,2012,103:58-64
- [4]Ma Y,Rajkumar M,Freitas H.Improvement of plant growth and nickel uptake by nickel resistant-plant growth promoting bacteria[J].Journal of Hazardous Materials,2009,166:1154-1161
- [5]Luo S L,Chen L,Chen J L,et al.Analysis and characterization of cultivable heavy metal-resistant bacterial endophytes isolated from Cd-hyperaccumulator Solanum nigrum L.and their potential use for phytoremediation[J].Chemosphere,2011,85:1130-1138
- [6]Zhang B L,Shang S H,Zhang H T,et al.Sodium chlorideenhances cadmium tolerance through reducing cadmium accumulation and increasing anti-oxidative enzyme activity in tobacco[J].Environmental Toxicology and Chemistry,2013,32(6):1420-1425
- [7]Sun L N,Zhang Y F,He L Y,et al.Genetic diversity and characterization of heavy metal-resistant-endophytic bacteria from two copper-tolerant plant species on copper mine wasteland[J].Bioresource Technology,2010,101:501-509
- [8]韩坤,田曾元,刘珂,等.具有ACC脱氨酶活性的海滨锦葵(Kosteletzkya pentacarpos)内生细菌对小麦耐盐性的影响[J].植物生理学报,2015,51(2):212-220Han K,Tian Z Y,Liu K,et al.Effect of endophytic bacteria with ACC deaminase activity in Kostel etzkya pentacarpos on wheat salt tolerance[J].Plant Physiology Journal,2015,51(2):212-220(in Chinese)
- [9]赵青云,赵秋芳,王辉,等.根际促生菌Bacillus subtilis Y-IVI在香草兰上的应用效果研究[J].植物营养与肥料学报,2015,21(2):535-540Zhao Q Y,Zhao Q F,Wang H,et al.Beneficial effects of plant growth promoter rhizobacteria on vanil a(Vanil a planifolia Ames.)growth[J].Journal of Plant Nutrition and Fertilizer,2015,21(2):535-540(in Chinese)
- [10]周小梅,赵运林,胥正钢,等.分泌吲哚乙酸的蒌蒿内生耐镉细菌的筛选与鉴定[J].生态环境学报,2014,23(12):1980-1985Zhou X M,Zhao Y L,Xu Z G,et al.Screening and identification of indole acetic acid-producing cadmium-resistant endophytic bacteria from Artemisia selengensis[J].Ecology and Environmental Sciences,2014,23(12):1980-1985(in Chinese)
- [11]梁泰帅,刘昌欣,康靖全,等.硫对镉胁迫下小白菜镉富集、光合速率等生理特性的影响[J].农业环境科学学报,2015,34(8):1455-1463Liang T S,Liu C X,Kang J Q,et al.Effects of sulfur on cadmium accumulation,photosynthesis and some other physiological characteristics of pakchoi(Brassica chinensis L.)under cadmium stress[J].Journal of Agro-Environment Science,2015,34(8):1455-1463(in Chinese)
- [12]李合生.植物生理生化实验原理和技术[M].北京:高等教育出版社,2000:165-170
- [13]Cakmak I,Marschner H.Magnesium deficiency and high light intensity enhance activities of superoxide dismutase,ascorbate peroxidase and glutathione reductase in bean leaves[J].Journal of Plant Physiology,1992,98:1222-1227
- [14]李振高,骆永明,滕应.土壤与环境微生物研究法[M].北京:科学出版社,2008:90-92
- [15]金香琴,杨萌尧,何春光,等.菲胁迫下湿生植物美人蕉(Canna indica)对斜生栅藻(Scenedesmus obliquus)生长的影响[J].生态毒理学报,2015,10(2):290-296Jin X Q,Yang M Y,He C G,et al.Effect of wetland plants Canna indica on the growth of Scenedesmus obliquus under phenanthrene stress[J].Asian Journal of Ecotoxicology,2015,10(2):290-296(in Chinese)
- [16]Gao Y G,Liu Q,Zang P,et al.An endophytic bacterium isolated from Panax ginseng C.A.Meyer enhances growth,reduces morbidity,and stimulates ginsenoside biosynthesis[J].Phytochemistry Letters,2015,11:132-138
- [17]刘俊祥,孙振元,勾萍,等.镉胁迫下多年生黑麦草的光合生理响应[J].草业学报,2012,21(3):191-197Liu J X,Sun Z Y,Gou P,et al.Response of photosynthetic physiology of perennial ryegrass(Lolium perenne)to Cd2+stress[J].Acta Prataculturae Sinica,2012,21(3):191-197(in Chinese)
- [18]Baryla A,Carrier P,Franck F,et al.Leaf chlorosis in oil seed rape plant(Brassica napus)grown on cadmium pol uted soil:Caused and consequences for photosynthesis and growth[J].Planta,2001,212:696-709
- [19]张晖,宋圆圆,吕顺,等.香蕉根际促生菌的抑菌活性及对作物生长的促进作用[J].华南农业大学学报,2015,36(3):65-70Zhang H,Song Y Y,Lv S,et al.The antifungal activity and crop growth stimulation of growth-promoting rhizobacteria from banana rhizosphere soil[J].Journal of South China Agricultural University,2015,36(3):65-70(in Chinese)
- [20]冯世鑫,莫长明,唐其,等.枯草芽孢杆菌肥在罗汉果上应用的效应分析[J].广西植物,2015,35(6):807-811Feng S X,Mo C M,Tang Q,et al.Effects of the bio-fertilizer of Bacillus subtilis on the application of Siraitia grosvenorii[J].Guihaia,2015,35(6):807-811(in Chinese)
- [21]贾新平,邓衍明,孙晓波,等.盐胁迫对海滨雀稗生长和生理特性的影响[J].草业学报,2015,24(12):204-212Jia X P,Deng Y M,Sun X B,et al.Impacts of salt stress on the growth and physiological characteristics of Paspalum vaginatum[J].Acta Prataculturae Sinica,2015,24(12):204-212(in Chinese)
- [22]张银秋,台培东,李培军,等.镉胁迫对万寿菊生长及生理生态特征的影响[J].环境工程学报,2011,5(1):195-200Zhang Y Q,Tai P D,Li P J,et al.Effects of cadmium stress on the growth and physiological characteristics of Tagetes erecta L.[J].Chinese Journal of Environmental Engineering,2011,5(1):195-200(in Chinese)
- [23]张怀山,赵桂琴,栗孟飞,等.中型狼尾草幼苗对PEG、低温和盐胁迫的生理应答[J].草业学报,2014,23(2):180-188Zhang H S,Zhao G Q,Li M F,et al.Physiological responses of Pennisetum longissimum var.intermedium seedlings to PEG,low temperature and salt stress treatments[J].Acta Prataculturae Sinica,2014,23(2):180-188(in Chinese)
- [24]詹淑威,潘伟斌,赖彩秀,等.外源有机酸对小飞扬草(Euphorbia thymifolia L.)修复镉污染土壤的影响[J].环境工程学报,2015,9(10):5096-5102Zhan S W,Fan W B,Lai C X,et al.Effects of exogenous organic acids on phytoremediation of Cd-contaminated soil by Euphorbia thymifolia L.[J].Chinese Journal of Environmental Engineering,2015,9(10):5096-5102(in Chinese)
- [25]Sheng X F,He L Y,Wang Q Y,et al.Effects of inoculation of biosurfactant-producing Bacillus sp.J119 on plant growth and cadmium uptake in a cadmium-amended soil[J].Journal of Hazardous Materials,2008,155:17-22
- [26]Ma Y,Rajkumar M,Freitas H.Isolation and characterization of Ni mobilizing PGPB from serpentine soils and their potential in promoting plant growth and Ni accumulation by Brassica sp.[J].Chemosphere,2009,75:719-725
- [27]Yuan B C,Yue D X.Soil microbial and enzymatic activities across a chronosequence of Chinese pine plantation development on the Loess Plateau of China[J].Pedosphere,2012,22(1):1-12
- [28]Ma Y H,Yin S L,Zhang L P,et al.Effects of complex microbial agent on microorganism population and soil enzyme activity in the cucumber rhizosphere soil[J].Plant Protection,2012,38(2):46-50
- [29]Caravaca F,Maboreke H,Kurth F,et al.Synergists and antagonists in the rhizosphere modulate microbial communities and growth of Quercus robur L.[J].Soil Biology&Biochemistry,2015,82:65-73