唐天乐;杨洋;陈亚文;朱小乔;唐文浩;刁晓平;

• 1:海南医学院热带医学与检验医学院
• 2:海南大学环境与植物保护学院
• 3:海南大学海口市环境毒理学重点实验室

摘要(Abstract)：

为评价双酚AF(BPAF)的甲状腺毒性,以雄性斑马鱼成鱼为受试模式生物,采用半静态染毒的方式对雄性斑马鱼成鱼染毒14 d(0、5、50和500μg·L~(-1)),分别设置7 d、14 d 2个时间节点,通过酶联免疫法(ELISA)分别检测斑马鱼血浆中总三碘甲腺原氨酸(TT3)、总甲状腺素(TT4)、游离三碘甲腺原氨酸(FT3)、游离甲状腺素(FT4)的含量水平,并对14 d暴露后斑马鱼甲状腺进行组织学检测分析。结果显示随着暴露浓度的提高,出现甲状腺滤泡上皮细胞增生,滤泡组织变形明显,细胞胶质缺失等病理学变化。雄性斑马鱼在BPAF暴露情况下,血浆中TT3、TT4、FT3、FT4含量随着暴露浓度的提升和时间的延长出现显著上升,呈现出甲状腺功能亢奋的干扰效应。

关键词(KeyWords)： 双酚AF;斑马鱼;甲状腺组织;甲状腺激素水平

Abstract:

Keywords:

基金项目(Foundation): 海南省科协青年科技人才学术创新计划项目(No.201513);; 海南医学院科研培育基金(No.HY2014-013)

作者(Author): 唐天乐;杨洋;陈亚文;朱小乔;唐文浩;刁晓平;

Email:

DOI:

参考文献(References)：

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