土壤酶和微生物量碳对土壤低浓度重金属污染的响应及其影响因子研究Response of Enzyme Activity and Microbial Biomass Carbon Content to Low Soil Heavy Metal Pollution and Soil Properties
线郁;王美娥;陈卫平;
摘要(Abstract):
土壤酶和微生物量碳是反映土壤健康的重要微生物性质指标。土壤重金属污染能够对土壤微生物性质产生影响,然而土壤酶和微生物量碳对土壤重金属污染的响应受到土壤本身理化性质的影响。通过北京市建成区233个样点的数据监测和实验室模拟,研究了土壤脲酶活性和微生物量碳对土壤低浓度重金属污染的响应以及受土壤理化性质的影响。研究结果表明:在野外主要重金属污染的浓度范围内(Cd 0.003~0.98μg·g-1,Cu 13.4~207.9μg·g-1,Zn 29.4~322μg·g-1,Pb 4.02~174μg·g-1),土壤脲酶活性、微生物量碳(MBC)和有机碳(SOC)的含量与土壤中Cd、Cu、Zn和Pb的浓度正相关,而微生物量碳占有机碳的比率(MBC/SOC)与重金属浓度负相关;脲酶活性、MBC/SOC与重金属浓度建立的相关关系只能解释总变异的5%~10%。实验室模拟试验表明,土壤酶活性受土壤重金属含量和土壤性质联合效应的影响;土壤有机质含量和pH是影响酶活性的主要土壤理化性质。引入土壤有机碳含量和pH两个参数,重新建立脲酶活性、MBC/SOC与土壤中重金属浓度的关系,建立的相关关系的决定系数变大,能够解释总变异的14%~17%。
关键词(KeyWords): 重金属污染;酶活性;微生物量碳;土壤有机质
基金项目(Foundation): 国家自然科学基金资助项目(41271503)
作者(Author): 线郁;王美娥;陈卫平;
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参考文献(References):
- [1]Balasubramanian A,Siddaramappa R,Rangaswami G.Effect of organic manuring on the activities of the enzymes hydrolysing sucrose and urea and on soil aggregation[J].Plant and Soil,1972,37(2):319-328
- [2]Brookes P C.The use of microbial parameters in monitoring soil pollution by heavy metals[J].Biology and Fertility of Soils,1995,19(4):269-279
- [3]Giller K E,Witter E,McGrath S P.Toxicity of heavy metals to microorganisms and microbial processes in agricultural soils:A review[J].Soil Biology and Biochemistry,1998,30(10-11):1389-1414
- [4]周启星,王美娥.土壤生态毒理学研究进展与展望[J].生态毒理学报2006,1(1):1-11Zhou Q X,Wang M E.Researching advancement and prospect of soil ecotoxicology[J].Asian Journal of Ecotoxicology,2006,1(1):1-11(in Chinese)
- [5]Chaperon S,Sauve S.Toxicity interaction of metals(Ag,Cu,Hg,Zn)to urease and dehydrogenase activities in soils[J].Soil Biology and Biochemistry,2007,39(9):2329-2338
- [6]Moreno J L,García C,Hernández T.Toxic effect of cadmium and nickel on soil enzymes and the influence of adding sewage sludge[J].European Journal of Soil Science,2003,54(2):377-386
- [7]Kandeler E,Tscherko D,Bruce K D,et al.Structure and function of the soil microbial community in microhabitats of a heavy metal polluted soi[J]l.Biology and Fertility of Soils,2000,32(5):390-400
- [8]Doelman P,Haanstra L.Short-and long-term effects of heavy metals on urease activity in soils[J].Biology and Fertility of Soils,1986,2(4):213-218
- [9]Sparling G.Ratio of microbial biomass carbon to soil organic carbon as a sensitive indicator of changes in soil organic matter[J].Soil Research,1992,30(2):195-207
- [10]Anderson T H,Domsch K H.Determination of ecophysiological maintenance carbon requirements of soil microorganisms in a dormant state[J].Biology and Fertility of Soils,1985,1(2):81-89
- [11]Mikanova O.Effects of heavy metals on some soil biological parameters[J].Journal of Geochemical Exploration,2006,88(1-3):220-223
- [12]Yang Y,Paterson E,Campbell C D.Urban soil microbial features and their environmental significance as exemplified by Aberdeen City,UK[J].Chinese Journal of Geochemistry,2001,20(1):34-44
- [13]Bhattacharyya P,Mitra A,Chakrabarti K,et al.Effect of heavy metals on microbial biomass and activities in century old landfill soil[J].Environmental Monitoring and Assessment,2008,136(1-3):299-306
- [14]Bastida F,Zsolnay A,Hernández T,et al.Past,present and future of soil quality indices:A biological perspective[J].Geoderma,2008,147(3 4):159-171
- [15]Karaca A,Cetin S C,Turgay O C,et al.Effects of heavy metals on soil enzyme activities[J].Soil Heavy Metals,2010,237-262
- [16]Moreno J L,Bastida F,Ros M,et al.Soil organic carbon buffers heavy metal contamination on semiarid soils:Effects of different metal threshold levels on soil microbial activity[J].European Journal of Soil Biology,2009,45(3):220-228
- [17]Kzlkaya R,Ak?n T,BayraklB,et al.Microbiological characteristics of soils contaminated with heavy metals[J].European Journal of Soil Biology,2004,40(2):95-102
- [18]Gillman G P,Bruce R C,Davey B G,et al.A comparison of methods used for determination of cation exchange capacity[J].Communications in Soil Science and Plant Analysis,1983,14(11):1005-1014
- [19]Nam J J,Thomas G O,Jaward F M,et al.PAHs in background soils from Western Europe:Influence of atmospheric deposition and soil organic matter[J].Chemosphere,2008,70(9):1596-1602
- [20]Frankeberger W,Johanson J.Method of measuring invertase activity in soils[J].Plant and Soil,1983,74(3):301-311
- [21]Gosewinkel U,Broadbent F.Conductimetric determination of soil urease activity[J].Communications in Soil Science&Plant Analysis,1984,15(11):1377-1389
- [22]Tabatabai M,Bremner J.Arylsulfatase activity of soils[J].Soil Science Society of America Journal,1970,34(2):225-229
- [23]Johnson J L,Temple K L.Some variables affecting the measurement of"catalase activity"in soil1[J].Soil Science Society of America Journal,1964,28(2):207-209
- [24]Stevenson I L.Dehydrogenase activity in soil[J].Canadian Journal of Microbiology,1959,5(2):229-235
- [25]Kramer M,Yerdei G.Application of the method of phosphatase activity determination in agricultural chemistry[J].Soviet Soil Science,1959,9:1100-1103
- [26]Vance E D,Brookes P C,Jenkinson D S.An extraction method for measuring soil microbial biomass C[J].Soil Biology and Biochemistry,1987,19(6):703-707
- [27]Wang M E,Markert B,Chen W P,et al.Identification of heavy metal pollutants using multivariate analysis and effects of land uses on their accumulation in urban soils in Beijing,China[J].Environmental Monitoring and Assessment,2012,184:5889-5897
- [28]Chander K,Brookes P C.Effects of heavy metals from past applications of sewage sludge on microbial biomass and organic matter accumulation in a sandy loam and a silty loam UK soil[J].Soil Biology and Biochemistry,1991,23(10):927-932
- [29]Zhang Y,Zhang H W,Su Z C,et al.Soil microbial characteristics under long-term heavy metal stress:A case study in Zhangshi Wastewater Irrigation Area,Shenyang[J].Pedosphere,2008,18(1):1-10
- [30]Yang R,Tang J,Chen X,et al.Effects of coexisting plant species on soil microbes and soil enzymes in metal lead contaminated soils[J].Applied Soil Ecology,2007,37(3):240-246
- [31]Khan S,Cao Q,Hesham A E-L,et al.Soil enzymatic activities and microbial community structure with different application rates of Cd and Pb[J].Journal of Environmental Sciences,2007,19(7):834-840
- [32]Wyszkowska J,Kucharski J,Lajszner W.The effects of copper on soil biochemical properties and its interaction with other heavy metals[J].Polish Journal of Environmental Studies,2006,15(6):927-934
- [33]Yang Z,Liu S,Zheng D,et al.Effects of cadium,zinc and lead on soil enzyme activities[J].Journal of Environmental Sciences,2006,18(6):1135-1141