董雪纯;魏海峰;刘长发;

• 1:辽宁省近岸海洋环境科学与技术重点实验室
• 2:大连海洋大学

摘要(Abstract)：

海洋中的多环芳烃(PAHs)具有较强的生物毒性,且海洋动物早期发育阶段是对环境因素变化响应的最敏感阶段。为探究海洋多环芳烃类有机污染物对仿刺参(Apostichopus japonicus)早期发育阶段原肠胚的毒性影响,采用半静态毒性实验方法,分别考察了4种多环芳烃苯并[a]芘、3-甲基菲、惹烯及2-甲基蒽对仿刺参原肠胚的24、48、72、96 h急性毒性效应。结果表明,在10、50、100、200μg·L~(-1)暴露浓度下,随着暴露时间的延长和暴露浓度的升高,4种多环芳烃对仿刺参原肠胚产生不同程度的急性毒性效应,仿刺参原肠胚存活率与4种多环芳烃浓度之间分别存在显著的剂量-效应关系(P<0.05)。苯并[a]芘对仿刺参原肠胚在24、48 h的半致死浓度(LC_(50))分别为294.4、225.64 mg·L~(-1),3-甲基菲在24、48、72、96 h的LC_(50)分别为404.5、300.7、81.4、17.6mg·L~(-1),惹烯在24、48、72 h的LC_(50)分别为243.1、230、186 mg·L~(-1),2-甲基蒽在24、48、72、96 h的LC_(50)分别244、231.6、152.6、142.9 mg·L~(-1)。4种多环芳烃的安全浓度(SC)分别为39.76、49.8、61.8、62.6μg·L~(-1),其毒性大小顺序为苯并[a]芘>3-甲基菲>惹烯>2-甲基蒽。基于定量构效关系(QSAR)的研究结果可知多环芳烃化合物的毒性差异可能与分子结构等性质有关。该实验为深入研究多环芳烃对海洋环境的毒性效应提供了理论依据。

关键词(KeyWords)： 多环芳烃;仿刺参;急性毒性;半致死浓度;安全浓度

Abstract:

Keywords:

基金项目(Foundation): 海洋公益性行业科研专项经费(No:201305002);; 国家海洋局海洋溢油鉴别与损害评估技术重点实验室开放课题(No:201309,201809);国家海洋局近岸海域生态环境重点实验室(No:201013);; 辽宁省教育厅服务地方项目(2017);; 大连海洋大学农业农村部北方海水增养殖重点实验室基金(No:2018-KF-21)

作者(Author): 董雪纯;魏海峰;刘长发;

Email:

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