韩笑笑;赵建;王震宇;隋海君;徐立娜;

• 1:中国海洋大学环境科学与工程学院
• 2:青岛海洋科学与技术国家实验室海洋生态与环境科学功能实验室

摘要(Abstract)：

为探讨老化时间对TiO_2纳米颗粒(nanoparticles,NPs)生物有效性的影响,研究了不同老化时间的Ti O_2NPs(0~120 d)对玉米幼苗生长的影响、在玉米体内的吸收及其在植株不同部位的存在位点等。研究发现,不同浓度的TiO_2NPs(1 000 mg·kg~(-1)和2 000 mg·kg~(-1))加入到土壤中,对玉米幼苗干鲜重没有明显的影响,但老化时间小于60 d时,对玉米幼苗株高有一定的抑制效应,老化60 d之后,随着老化时间的继续延长,毒性逐渐降低,最后趋于稳定。老化60 d时,TiO_2NPs处理的玉米幼苗根冠增大,玉米幼苗体内产生H2O_2的累积。在Ti O_2老化土壤中生长的玉米幼苗根系和地上部均有Ti的累积,1 000 mg·kg~(-1)的TiO_2NPs在玉米幼苗根部的生物累积系数达到35.4%,在地上部为13.6%,在玉米植株体内的转运系数为0.38;通过TEM观察,TiO_2NPs可以进入到玉米幼苗体内,并存在于根细胞的细胞质和叶绿体膜上,在叶片细胞的液泡和细胞核中也发现有TiO_2NPs的存在。上述研究结果为客观评价TiO_2NPs的生态风险提供了有用信息。

关键词(KeyWords)： TiO2NPs;玉米幼苗;生长效应;吸收转运

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(41073067;41120134004)

作者(Author): 韩笑笑;赵建;王震宇;隋海君;徐立娜;

Email:

DOI:

参考文献(References)：

• [1]王震宇,赵建,李娜,等.人工纳米颗粒对水生生物的毒性效应及其机制研究进展[J].环境科学,2010,30(8):1409-1418Wang Z Y,Zhao J,Li N,et al.Review of ecotoxicity and mechanism of engineered nanoparticless to aquatic organisms[J].Environmental Science,2010,30(8):1409-1418(in Chinese)
• [2]白伟,张程程,姜文君,等.纳米材料的环境行为及其毒理学研究进展[J].生态毒理学报,2009,4(2):174-182Bai W,Zhao C C,Jiang W J,et al.Progress instudies on environmental behaviors and toxicological effects of nanomaterials[J].Asian Journal of Ecotoxicology,2009,4(2):174-182(in Chinese)
• [3]Lovern S,Klaper R.Daphnia magna mortality when exposed to titanium dioxide and fullerene(C60)nanoparticles[J].Environmental Toxicology and Chemistry,2006,25:1132-1137
• [4]Jiang W,Mashayekhi H,Xing B.Bacterial toxicity comparison between nano-and micro-scaled oxide particles[J].Environmental Pollution,2009,157:1619-1625
• [5]Hund-Rinke K,Simon M.Ecotoxic effect of photocatalytic active nanoparticles(Ti O2)on algae and daphnia[J].Environmental Science and Pollution Research,2006,13:225-232
• [6]Zhao J,Wang Z,Liu X,et al.Distribution of Cu O nanoparticles in juvenile carp(Cyprinus carpio)and their potential toxicity[J].Journal of Hazardous Materials,2011,197:304-310
• [7]金盛杨,王玉军,汪鹏,等.不同培养介质中纳米氧化铜对小麦毒性的影响[J].生态毒理学报,2010,5(6):842-848Jin S Y,Wang Y J,Wang P,et al.Influence of culturemedia on the phytotoxicity of Cu O nanoparticles to wheat(Triticum aestivum L.)[J].Asian Journal of Ecotoxicology,2010,5(6):842-848(in Chinese)
• [8]Zhao L,Sun Y,Hernandez-Viezcas J,et al.Monitoring the environmental effects of Ce O2and Zn O nanoparticles through the life cycle of corn(Zea mays.L)plants and in situμ-XRF mapping of nutrients in kernels[J].Environmental Science&Technology,2015,49(5):2921-2928
• [9]Wang Z,Xie X,Xing B,et al.Xylem-and phloem-based transport of Cu O nanoparticles in maize(Zea mays L.)[J].Environmental Science&Technology,2012,46(8):4434-4441
• [10]Pereira R,Rocha-Santos P,Antunes E,et al.Screening evaluation of the ecotoxicity and genotoxicity of soils contaminated with organic and inorganic nanoparticles:The role of aging[J].Journal of Hazardous Materials,2011,194:345-354
• [11]Lee W M,An Y J,Yoon H.Toxicity and bioavailability of copper nanoparticles to the terrestrial plant mung bean(Phaseolus radiatus)and w heat(Triticum aestivum):Plant agar test for w ater-insoluble nanoparticles[J].Environmental Toxicology and Chemistry,2008,27(9):1915-1921
• [12]Paula S,Cornelis A,Stephen L,et al.Metal-based nanoparticles in soil:Fate,behavior and effects on soil invertebrates[J].Environmental Toxicology and Chemistry,2012,31:1679–1692
• [13]Ma Y H,Kuang L L,He X,et al.Effects of rare earth oxide nanoparticles on root elongation of plants[J].Chemosphere,2010,78(3):273-279
• [14]王震宇,于晓莉,高冬梅,等.人工合成纳米Ti O2和MWCNTs对玉米生长及抗氧化系统的影响[J].环境科学,2010,31(2):480-487Wang Z Y,Yu X L,Gao D M,et al.Effect of nano-rutile Ti O2and multiwalled carbon nanotubes on growth of maize(Zea may L.)seedlings and the relevant antioxidant response[J].Environmental Science,2010,31(2):480-487(in Chinese)
• [15]陈花,吴俊林,李晓军.叶绿体中活性氧的产生和清除机制[J].现代生物医学进展,2008(8):1979-1983Chen H,Wu J L,Li X J.Generation and scavenging of reactive oxygen species in chloroplast[J].Progress in Modern Biomedicine,2008(8):1979-1983(in Chinese)
• [16]徐立娜,赵建,王震宇.Cu O纳米颗粒对拟南芥叶片生长及生理特性的影响[J].植物生理学报,2015,51(6):955-961Xu L N,Zhao J,Wang Z Y.Effect of Cu O nanoparticles on growth and physiological characteristics in leaf of Arabidopsis thaliana[J].Plant Physiology Journal,2015,51(6):955-961(in Chinese)
• [17]Du W C,Sun Y Y,Rong J,et al.Ti O2and Zn O nanoparticles negatively affect wheat growth and soil enzyme activities in agricultural soil[J].Journal of Environmental Monitoring,2011,13:822-828
• [18]Lin D H,Xing B S.Root uptake and phytotoxicity of Zn O nanoparticles[J].Environmental Science&Technology,2008,42(15):5580-5585