三氯生对青海弧菌Q67和人乳腺癌细胞MCF-7的时间毒性Time-dependent Toxicities of Triclosan to Vibrio qinghaiensis sp.-Q67 and Human Breast Cancer Cell MCF-7
李晓磊;刘树深;朱祥伟;张瑾;葛会林;
摘要(Abstract):
三氯生(triclosan,TCS)是一种广谱性抗菌剂,2005年欧盟水框架指令将TCS列为一种新型污染物。目前对TCS的研究局限于急性毒性实验,关于TCS毒性随时间的变化以及不同溶解状态下TCS的毒性差异的研究却鲜有报道。应用以96孔微板为暴露反应载体的微板毒性分析法,添加氢氧化钠(NaOH)或使用二甲亚砜(DMSO)作为助溶剂溶解TCS,分别测定其对青海弧菌Q67的相对发光抑制毒性(15min急性毒性和时间毒性)和对人乳腺癌细胞MCF-7在不同暴露时间(24、48和72h)内的细胞增殖抑制毒性。Q67的急性毒性实验结果表明,碱性条件下TCS的毒性(EC50=3.97(10-8mol.L-1)大于DMSO作为助溶剂时的毒性(EC50=1.68(10-4mol.L-1)。无论碱性条件还是DMSO助溶,TCS在不同暴露时间内对Q67的时间毒性没有明显差异。在不同暴露时间下MCF-7增殖抑制率实验中,DMSO作为助溶剂时,TCS的最高实验浓度为1.46(10-3mol.L-1,随着暴露时间的延长,抑制率在24、48和72h时分别为27.8%、44.2%和62.4%;碱性环境时TCS的最高实验浓度为1.39(10-6mol.L-1,随着暴露时间的延长,抑制率在24、48和72h时分别为20.2%、55.8%和73.9%。研究表明,在DMSO和NaOH作为助溶剂的条件下,TCS对MCF-7均存在时间毒性差异,并且NaOH碱性溶液中TCS对MCF-7的毒性远大于DMSO作为助溶剂时的毒性。
关键词(KeyWords): 三氯生;青海弧菌;MCF-7细胞;时间毒性
基金项目(Foundation): 国家自然科学基金项目(20977065);; 国家高技术研究发展计划(863)项目(2007AA06Z417)
作者(Author): 李晓磊;刘树深;朱祥伟;张瑾;葛会林;
Email:
DOI:
参考文献(References):
- [1]McAvoy D C,Schatowitz B,Jacob M,et al.Measure-ment of triclosan in wastewater treatment systems[J].Environmental Toxicology and Chemistry,2002,21(7):1323-1329
- [2]Brun E M,Puchades R,Bonet E,et al.Selective en-zyme-linked immunosorbent assay for triclosan.Ap-plication to wastewater treatment plant effluents[J].Environmental Science&Technology,2008,42(5):1665-1672
- [3]Coogan M A,Edziyie R E,La Point T W,et al.Algal bioaccumulation of triclocarban,triclosan,and methyl-tri-closan in a North Texas wastewater,treatment plant re-ceiving stream[J].Chemosphere,2007,67(10):1911-1918
- [4]Kolpin D W,Furlong E T,Meyer M T,et al.Pharma-ceuticals,hormones,and other organic wastewater con-taminants in US streams,1999—2000:A national recon-naissance[J].Environmental Science&Technology,2002,36(6):1202-1211
- [5]Mezcua M,Gomez M J,Ferrer I,et al.Evidence of2,7/2,8-dibenzodichloro-p-dioxin as a photodegradation prod-uct of triclosan in water and wastewater samples[J].A-n alytica Chimica Acta,2004,524(1-2):241-247
- [6]Calafat A M,Ye X,Wong L Y,et al.Urinary concentra-tions of triclosan in the US population:2003—2004[J].Environmental Health Perspectives,2008,116(3):303-307
- [7]Nassef M,Matsumoto S,Seki M,et al.Acute effects of triclosan,diclofenac and carbamazepine on feeding per-formance of Japanese medaka fish(Oryzias latipes)[J].Chemosphere,2010,80(9):1095-1100
- [8]DeLorenzo M E,Keller J M,Arthur C D,et al.Toxicity of the antimicrobial compound triclosan and formation of the metabolite methyl-triclosan in estuarine systems[J].Environmental Toxicology,2008,23(2):224-232
- [9]Ying G G,Yang L H,Su H C,et al.Growth-inhibitingeffects of12antibacterial agents and their mixtures on the freshwater microalga Pseudokirchneriella subcapitata[J].Environmental Toxicology and Chemistry,2008,27(5):1201-1208
- [10]Zhang Y H,Liu S S,Song X Q,et al.Prediction for the mixture toxicity of six organophosphorus pesticides to the luminescent bacterium Q67[J].Ecotoxicology and Environmental Safety,2008,71(3):880-888
- [11]Andersen H R,Vinggaard A M,Rasmussen T H,et al.Effects of currently used pesticides in assays for estroge-nicity,androgenicity,and aromatase activity in vitro[J].Toxicology and Applied Pharmacology,2002,179(1):1-12
- [12]刘保奇,葛会林,刘树深.测定环境污染物对青海弧菌发光强度抑制的微板发光方法研究[J].生态毒理学报,2006,1(2):186-191Liu B Q,Ge H L,Liu S S.Microplate luminometry for toxicity bioassay of environmental pollutant on a new type of fresh water luminescent bacterium(Vibrio-qing-haiensissp.-Q67)[J].Asian Journal of Ecotoxicology,2006,1(2):186-191(in Chinese)
- [13]Zhang J,Liu S S,Liu H L,et al.A novel method depend-ent only on the mixture information(MIM)for evaluating the toxicity of mixture[J].Environmental Pollution,2011,159(7):1941-1947
- [14]Dou R N,Liu S S,Mo L Y,et al.A novel direct equipar-tition ray design(EquRay)procedure for toxicity interac-tion between ionic liquid and dichlorvos[J].Environmen-tal Science and Pollution Research,2011,18(5):734-742
- [15]Zhang J,Liu S S,Liu H L.Effect of ionic liquid on the toxicity of pesticide to Vibrio-qinghaiensis sp.-Q67[J].Journal of Hazardous Materials,2009,170(2-3):920-927
- [16]Fan F,Rozman K K.Short-term and long-term biochemi-cal effects of2,3,7,8-tetrachlorodibenzo-P-dioxin in fe-male long-evans rats[J].Toxicology Letters,1995,75(1-3):209-216
- [17]Backhaus T,Froehner K,Altenburger R,et al.Toxicity testing with Vibrio fischeri:A comparison between the long term(24h)and the short term(30min)bioassay[J].Chemosphere,1997,35(12):2925-2938
- [18]Schmitt H,Haapakangas H,van Beelen P.Effects of anti-biotics on soil microorganisms:Time and nutrients influ-ence pollution-induced community tolerance[J].Soil Bi-ology&Biochemistry,2005,37(10):1882-1892
- [19]Zhu X W,Liu S S,Ge H L,et al.Comparison between the short-term and the long-term toxicity of six triazine herbicides on photobacteria Q67[J].Water Research,2009,43(6):1731-1739
- [20]李林朋,马慧敏,胡俊杰,等.三氯生和三氯卡班对人体肝细胞DNA损伤的研究[J].生态环境学报,2010,19(12):2897-2901Li L P,Ma H M,Hu J J,et al.The genotoxicity of tri-closan and triclocarban in human hepatocyte L02cell[J].Ecology and Environmental Sciences,2010,19(12):2897-2901(in Chinese)
- [21]Bhargava H N,Leonard P A.Triclosan:Applications and safety[J].American Journal of Infection Control,1996,24(3):209-218
- [22]Malich G,Markovic B,Winder C.The sensitivity and specificity of the MTS tetrazolium assay for detecting the in vitro cytotoxicity of20chemicals using human cell lines[J].Toxicology,1997,124(3):179-192
- [23]Tanos V,Brzezinski A,Drize O,et al.Synergistic inhibi-tory effects of genistein and tamoxifen on human dys-plastic and malignant epithelial breast cells in vitro[J].European Journal of Obstetrics Gynecology and Repr-o ductive Biology,2002,102(2):188-194