载带苯并[a]芘的纳米碳/二氧化硅颗粒引起大鼠氧化应激作用的研究Study on the Oxidative Stress of Rats Induced by Benzo[a]pyrene Coated onto Nano Carbon/Silicon Dioxide Particles
张华山;杨丹凤;袭著革;杨在明;刘焕亮;李君文;
摘要(Abstract)：
通过人工制备载带B[a]P的纳米碳(C)和纳米二氧化硅(SiO2)颗粒,采用气管滴注染毒方式,以7.5mg·kg-1(以体重计)的染毒剂量急性染毒大鼠,观察染毒24小时后载带B[a]P的纳米C/SiO2颗粒对机体产生氧化应激损伤的联合毒性效应.结果表明,在急性染毒后大鼠外周血中反映机体脂质过氧化损伤程度指标的丙二醛(MDA)含量表现为染毒组较对照组显著增加(p<0.05),表明纳米颗粒诱发机体发生了氧化应激反应.在急性染毒后各组大鼠肺泡灌洗液中谷胱苷肽过氧化物酶(GSH-PX)和超氧化物岐化酶(T-SOD)活力与对照组相比显著增加(p<0.05)(载带B[a]P的纳米SiO2组除外);载带B[a]P的纳米SiO2组肺泡灌洗液中GSH-PX活力与对照组相比无显著差异,而与单纯纳米SiO2组和B[a]P组比较显著降低,推测与抗氧化酶的一过性增高有关;载带B[a]P的纳米C组肺泡灌洗液T-SOD活力与其单纯纳米C组和单纯B[a]P组比较显著增加(p<0.05),由此表明载带B[a]P的复合纳米C/SiO2颗粒在致机体氧化损伤效应方面二者存在一定的协同作用.
关键词(KeyWords)： 纳米碳颗粒;纳米二氧化硅颗粒;苯并[a]芘;氧化应激
基金项目(Foundation): 国家863青年基金项目(No.2004AA649170)
作者(Author): 张华山;杨丹凤;袭著革;杨在明;刘焕亮;李君文;
Email:
DOI:
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