张博文;赵甲亭;吴二威;李云云;李娜;李柏;乔秀文;吴刚;高愈希;

• 1:中国科学院高能物理研究所纳米生物效应及安全性重点实验室
• 2:包头医学院基础医学与法医学院
• 3:石河子大学化学化工学院新疆兵团化工绿色过程重点实验室

摘要(Abstract)：

为调查滏阳河水系的重金属污染状况,研究河流重金属污染对水生生物的毒性,根据河流结构、水文条件、排污口分布并考虑空间分布的均匀性,选取66个采样位点,采集河水及对应的表层沉积物样品,分析了样品中的重金属含量。用斜生栅藻和青海弧菌Q67作为模式生物,根据滏阳河水重金属污染较严重的邯郸近郊2号采样点采集水样的重金属含量配制系列重金属浓度的模拟河水进行重金属污染河水的生态毒性测试。结果表明,在全部66个采样点中,29个采样点河水重金属含量超过国家地表水III类水体重金属含量标准,主要污染元素是Hg、Pb、Cr、Zn。几乎所有采样点河水Mn和Fe含量都大大高于国家集中式生活饮用水地表水源地补充项目规定的标准限值。根据2号采样点河水样品中的重金属含量配成的模拟河水对青海弧菌Q67的EC_(50)值为6.65%,为毒性极强的污染物。模拟河水样品对斜生栅藻的抑制作用较小,在实验的最高浓度下(1 000倍河水重金属含量)暴露4 d尚未引起半数藻细胞死亡。随河水重金属浓度上升,斜生栅藻超氧化物歧化酶(SOD)活性总体上呈现先升高后降低的趋势,丙二醛(MDA)含量变化则与此相反,反映河水重金属污染可引起藻细胞的氧化损伤。叶绿素a和b含量则随暴露浓度的提高逐渐降低。在重金属浓度达到2号采样点河水的10%时,斜生栅藻叶绿素a含量已有显著降低,MDA含量显著升高,海河流域重金属污染对生态系统的影响应予以重视。河水发光菌Q67的生长抑制率、斜生栅藻的叶绿素a和MDA含量可以作为评估河流重金属污染生态危害的指标。

关键词(KeyWords)： 滏阳河;重金属;青海弧菌Q67;斜生栅藻;生态毒性

Abstract:

Keywords:

基金项目(Foundation): 国家水体污染控制与治理科技重大专项课题(2012ZX07203-006)

作者(Author): 张博文;赵甲亭;吴二威;李云云;李娜;李柏;乔秀文;吴刚;高愈希;

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DOI:

参考文献(References)：

• [1]王金霞,黄季煜.滏阳河流域的水资源问题[J].自然资源学报,2004,19(4):424-429Wang J X,Huang J Y.Water issues in the Fuyang River basin[J].Journal of Natural Resources,2004,19(4):424-429(in Chinese)
• [2]李亚楠,孙宝盛,张燕.海河流域水质评价与预测[J].水土保持通报,2014,34(2):177-181Li Y N,Sun B S,Zhang Y.Evaluation and prediction of water quality in Haihe River basin[J].Bulletin of Soil and Water Conservation,2014,34(2):177-181(in Chinese)
• [3]Niu L L,Yang F X,Xu C,et al.Status of metal accumulation in farmland soils across China:From distribution to risk assessment[J].Environmental Pollution,2013,176:55-62
• [4]张淑娜,刘伟,王德龙.海河干流(市区段)表层沉积物重金属污染及变化趋势分析[J].干旱环境监测,2008,22(3):129-133Zhang S N,Liu W,Wang D L.Heavy metal pollution and their history trend in the surface sediment of Haihe River[J].Arid Environmental Monitoring,2008,22(3):129-133(in Chinese)
• [5]刘清,马梅,童中华,等.Cu、Zn、Cd、Hg对青海弧菌(Q67菌株)联合毒性作用的研究[J].中国环境科学,1997,17(4):301-303Liu Q,Ma M,Tong Z H,et al.Combined toxicity of Cu,Zn,Cd,Hg to Vibrio qinghaiensis sp.nov.(strain Q67)[J].China Environmental Science,1997,17(4):301-303(in Chinese)
• [6]刘保奇,葛会林,刘树深.测定环境污染物对青海弧菌发光强度抑制的微板发光法研究[J].生态毒理学报,2006,1(2):186-191Liu B Q,Ge H L,Liu S S.Microplate luminometry for toxicity bioassay of environmental pollutant on a new type of fresh water luminescent bacterium(Vibrio qinghaiensis sp.-Q67)[J].Asian Journal of Ecotoxicology,2006,1(2):186-191(in Chinese)
• [7]徐晓宇,魏颖,唐红枫,等.甲胺磷、氯氰菊酯及其混合物对斜生栅藻的毒性[J].农业环境科学学报,2007,26(2):718-722Xu X Y,Wei Y,Tang H F,et al.Combined toxicity effect of methamiclophos and cypermethrin to Scenedesmus obliquus[J].Journal of Agro-Environment Science,2007,26(2):718-722(in Chinese)
• [8]胡芹芹,熊丽,田裴秀子,等.邻苯二甲酸二丁酯(DBP)对斜生栅藻的致毒效应研究[J].生态毒理学报,2008,3(1):87-92Hu Q Q,Xiong L,Tianpei X,et al.Toxic effect s of dibutyl phthalate(DBP)on Scenedesmus obliquus[J].Asian Journal of Ecotoxicology,2008,3(1):87-92(in Chinese)
• [9]郎超,单保庆,李思敏,等.滏阳河表层沉积物重金属污染现状分析及风险评价[J].环境科学学报,2016,36(1):64-73Lang C,Shan B Q,Li S M,et al.Pollution analysis and ecological risk assessment of heavy metals in surface sediments of Fuyang River[J].Acta Scientiae Circumstantiae,2016,36(1):64-73(in Chinese)
• [10]李珊珊,单保庆,张洪.滏阳河河系表层沉积物重金属污染特征及其风险评价[J].环境科学学报,2013,33(8):2277-2284Li S S,Shan B Q,Zhang H.Characteristics and ecological risk assessment of heavy metal pollution in surface sediments of Fuyang River[J].Acta Scientiae Circumstantiae,2013,33(8):2277-2284(in Chinese)
• [11]Tang W Z,Zhang H,Shan B Q,et al.Accumulation and risk assessment of sedimentary trace metals in response to industrialization from the tributaries of Fuyang River system[J].Environmental Earth Sciences,2015,73:1975-1982
• [12]邓辅财,刘树深,刘海玲,等.部分重金属化合物对淡水发光菌的毒性研究[J].生态毒理学报,2007,2(4):402-408Deng F C,Liu S S,Liu H L,et al.Toxicities of selected heavy metal compounds and their mixtures to photobacteria(Vibrio qinghaiensis sp.-Q67)[J].Asian Journal of Ecotoxicology,2007,2(4):402-408(in Chinese)
• [13]Ma M,Tong Z,Wang Z,et al.Acute toxicity bioassay using the fresh water luminescent bacterium Vibrio qinghaiensis sp.nov.-Q67[J].Bulletin of Environmental Contamination and Toxicology,1999,62(3):247-253(in Chinese)
• [14]皇甫鑫,廖翀,杨坪,等.金属化合物对发光菌的毒性效应及不同发光菌的敏感度差异研究[J].环境科学学报,2010,30(9):1787-1792Huangfu X,Liao C,Yang P,et al.Toxicity of metal compounds to luminescent bacteria and sensitivity differences of different luminescent bacteria[J].Acta Scientiae Circumstantiae,2010,30(9):1787-1792(in Chinese)
• [15]周世明,赵清,舒为群.青海弧菌Q67新鲜培养菌液测试水中砷铬铅镉汞的急性毒性[J].预防医学情报杂志,2008,24(6):403-406Zhou S M,Zhao Q,Shu W Q.Use freshly prepared Vibrio qinghaiensis sp.-Q67 suspension to detect acute toxicity of metal chemicals in water[J].Journal of Preventive Medicine Information,2008,24(6):403-406(in Chinese)