邻苯二甲酸二乙基己酯(DEHP)对小鼠的氧化损伤及黄豆异黄酮对其保护作用的研究(英文)Oxidative Damage Induced by Di-(2-ethylhexyl) Phthalate and Protective Effects of Soybean Isoflavones in Mice
王莉;郭靖;韩冰;秦龙娟;杨旭;
摘要(Abstract):
邻苯二甲酸二乙基己酯(DEHP)是环境中广泛存在的污染物,作为一种内分泌干扰物,已严重威胁到人类健康和生态环境安全。每天用250、500和1000mg·kg-1·d-1的DEHP对雄性昆明小鼠进行灌胃处理,连续处理30d后,分别考察了小鼠的器官相对质量、丙二醛(MDA)和谷胱甘肽(GSH)的含量以及睾丸组织学等指标的变化。结果显示,小鼠的肝、肺组织肿大,睾丸严重萎缩,并呈剂量依赖性。肝脏MDA含量显著增加,肝脏和血液GSH水平显著下降。从睾丸组织学分析结果可见,精子生长发育条件已受到破坏,例如睾丸细胞坏死和凋亡,生精小管上皮细胞大量脱落等。DEHP对未成熟的雄性小鼠有氧化损伤作用和生殖毒性,特别是在较高剂量(500and1000mg·kg-1·d-1)时。为了探讨大豆异黄酮(SI)对DEHP诱导的氧化损伤和睾丸损伤的保护作用,将暴露于1000mg·kg-1·d-1DEHP的小鼠,用100mg·kg-1·d-1剂量的SI平行处理。结果显示,SI作为抗氧化剂和雌激素调节剂可明显减弱DEHP所致的氧化应激和睾丸毒性。
关键词(KeyWords): 邻苯二甲酸二乙基己酯(DEHP);氧化损伤;睾丸损伤;黄豆异黄酮;抗氧化剂;雌激素调节
基金项目(Foundation): 国家自然科学基金面上项目(51076079)
作者(Authors): 王莉;郭靖;韩冰;秦龙娟;杨旭;
参考文献(References):
- [1]Kavlock R,Barr D,Boekelheide K,et al.NTP-CERHR expert panel update on the reproductive and developmental toxicity of di-(2-ethylhexyl)phthalate[J].Reproductive Toxicology,2006,22(3):291-399
- [2]Tickner ScD J A,Schettler MD T,Guidotti MD T,et al.Health risks posed by use of di-2-ethylhexyl phthalate(DEHP)in PVC medical devices:A critical review[J].American Journal of Industrial Medicine,2001,39(1):100-111
- [3]Shea K M.Pediatric exposure and potential toxicity of phthalate plasticizers[J].Pediatrics,2003,111(6):1467-1474
- [4]Seo K W,Kim K B,Kim Y J,et al.Comparison of oxidative stress and changes of xenobiotic metabolizing enzymes induced by phthalates in rats[J].Food and Chemical Toxicology,2004,42(1):107-114
- [5]Latini G,Verrotti A,De Felice C.Di-2-ethylhexyl phthalate and endocrine disruption:A review[J].Current Drug Targets-Immune,Endocrine&Metabolic Disorders,2004,4(1):37-40
- [6]Dalgaard M,Nellemann C,Lam H R,et al.The acute effects of mono(2-ethylhexyl)phthalate(MEHP)on testes of prepubertal Wistar rats[J].Toxicology Letters,2001,122(1):69-79
- [7]Eveillarda A,Lasserrea F,de Tayrac M,et al.Identification of potential mechanisms of toxicity after di-(2-ethylhexyl)-phthalate(DEHP)adult exposure in the liver using a systems biology approach[J].Toxicology and Applied Pharmacology,2009,236(3):282-292
- [8]Dalsenter P R,Santana G M,Grande S W,et al.Phthalate affect the reproductive function and sexual behavior of male Wistar rats[J].Human&Experimental Toxicology,2006,25(6):297-303
- [9]Suna S,Yamaguchi F,Kimura S,et al.Preventive effect of d-psicose,one of rare ketohexoses,on di-(2-ethylhexyl)phthalate(DEHP)-induced testicular injury in rat[J].Toxicology Letters,2007,173(2):107-117
- [10]Latini G,Del Vecchio A,Massaro M,et al.Phthalate exposure and male infertility[J].Toxicology,2006,226(2-3):90-98
- [11]Reinli K,Block G.Phytoestrogen content of foods—A compendium of literature values[J].Nutrition and Cancer,1996,26(2):123-148
- [12]Akitha Devi M K,Gondi M,Sakthivelu G,et al.Functional attributes of soybean seeds and products,with reference to isoflavone content and antioxidant activity[J].Food Chemistry,2009,114(3):771-776
- [13]Hsieh H M,Wu W M,Hu M L.Soy isoflavones attenuate oxidative stress and improve parameters related to aging and Alzheimer's disease in C57BL/6J mice treated with D-galactose[J].Food and Chemical Toxicology,2009,47(3):625-632
- [14]Rimbach G,Boesch-Saadatmandi C,Frank J,et al.Dietary isoflavones in the prevention of cardiovascular disease—A molecular perspective[J].Food and Chemical Toxicology,2008,46(4):1308-1319
- [15]Watanabe S,Uesugi S,Kikuchi Y.Isoflavones for prevention of cancer,cardiovascular diseases,gynecological problems and possible immune potentiation[J].Biomedicine&Pharmacotherapy,2002,56(6):302-312
- [16]Occhiuto F,Zangla G,Samperi S,et al.The phytoestrogenic isoflavones from Trifolium pratense L.(Red clover)protects human cortical neurons from glutamate tox-icity[J].Phytomedicine,2008,15(9):676-682
- [17]Faqi A S,Johnson W D,Morrissey R L,et al.Reproductive toxicity assessment of chronic dietary exposure to soy isoflavones in male rats[J].Reproductive Toxicology,2004,18(4):605-611
- [18]Guan L,Huang Y,Chen Z Y.Developmental and reproductive toxicity of soybean isoflavones to immature SD rats[J].Biomedical and Environmental Sciences,2008,21(3):197-204
- [19]Cederroth C R,Nef S.Soy,phytoestrogens and metabolism:A review[J].Molecular and Cellular Endocrinology,2009,304(1-2):30-42
- [20]Kavlock R,Boekelheide K,Chapin R,et al.NTP Center for the evaluation of risks to human reproduction:Phthalates expert panel report on the reproductive and developmental toxicity of di(2-ethylhexyl)phthalate[J].Reproductive Toxicology,2002,16(5):529-653
- [21]Ishihara M,Itoh M,Miyamoto K,et al.Spermatogenic disturbance induced by di-(2-ethylhexyl)phthalate is significantly prevented by treatment with antioxidant vitamins in the rat[J].International Journal of Andrology,2000,23(2):85-94
- [22]Ruiz-Larrea M B,Mohan A R,Paganda G,et al.Antioxidant activity of phytoestrogenic isoflavones[J].Free Radical Research,1997,26(1):63-70
- [23]Patel R P,Boersma B J,Crawford J H,et al.Antioxidant mechanism of isoflavones in lipid system:Paradoxical effects of peroxyl radical scavenging[J].Free Radical Biology and Medicine,2001,31(12):1570-1581
- [24]Kireev R A,Tresguerres A F,Vara E,et al.Effect of chronic treatments with GH,melatonin,estrogens,and phytoestrogens on oxidative stress parameters in liver from aged female rats[J].Biogerontology,2007,8(5):469-482
- [25]Hwang J,Sevanian A,Hodis H N,et al.Synergistic inhibition of LDL oxidation by phytoestrogens and ascorbic acid[J].Free Radical Biology and Medicine,2000,29(1):79-89
- [26]Park J D,Habeebu S S M,Klaassen C D.Testicular toxicity of di-(2-ethylhexyl)phthalate in young Sprague-Dawley rats[J].Toxicology,2002,171(2-3):105-115
- [27]Svechnikov K,Svechnikova I,Sder O.Inhibitory effects of mono-ethylhexyl phthalate on steroidogenesis in immature and adult rat Leydig cells in vitro[J].Reproductive Toxicology,2008,25(4):485-490
- [28]Kasahara E,Sato E F,Miyoshi M,et al.Role of oxidative stress in germ cell apoptosis induced by di(2-ethylhexyl)phthalate[J].Biochemical Journal,2002,365(3):849-856
- [29]Lambrinidis G,Halabalaki M,Katsanou E S,et al.The estrogen receptor and polyphenols:Molecular simulation studies of their interactions,a review[J].Environmental Chemistry Letters,2006,4(3):159-174
- [30]Barone M,Tanzi S,Lofano K,et al.Estrogens,phytoestrogens and colorectal neoproliferative lesions[J].Genes&Nutrition,2008,3(1):7-13
- [31]Wiseman H.The bioavailability of non-nutrient plants factors:Dietary flavonoids and phyto-oestrogens[J].Proceedings of the Nutrition Society,1999,58(1):139-146
- [32]Xu X,Harris K S,Wang H J,et al.Bioavailability of soybean isoflavones depends upon gut microflora in women[J].Journal of Nutrition,1995,125(9):2307-2315