叶婷;康美;黄乾生;董四君;

• 1:中国科学院城市环境研究所中国科学院城市环境与健康重点实验室

摘要(Abstract)：

为探讨邻苯二甲酸二(2-乙基己)酯(DEHP)和邻苯二甲酸单乙基己基酯(MEHP)长期暴露对海水生物的内分泌干扰效应及机制,将孵化后1周的海洋青鳉(Oryzias melastigma)分别暴露于DEHP(0.1 mg·L-1和0.5 mg·L-1)和MEHP(0.1 mg·L-1和0.5 mg·L-1)6个月。结果显示:DEHP显著增加了雌性和雄性海洋青鳉的肝指数,而MEHP只在高剂量时显著增加雄性青鳉的肝指数。对于雌性青鳉,DEHP暴露后肝脏雌激素相关基因ERα、ERβ、ERγ、VTG1、VTG2、ChgH和ChgL的表达水平显著上调,而对于雄性青鳉,DEHP暴露后,只有肝脏ERβ的表达水平显著上调。相比之下,MEHP暴露对雌性和雄性青鳉肝VTG和Chg基因表达无显著影响。DEHP激活了雌性和雄性青鳉的肝过氧化物增殖激活受体PPARα和PPARγ,而MEHP只在低剂量时上调了雄性青鳉PPARγ的表达。在雌性和雄性青鳉体内,VTG和Chg的表达与ERα和ERγ的表达显著相关,并且ER与PPAR也显著相关。研究表明,DEHP长期暴露可通过激活肝性激素受体调控肝雌激素响应基因(VTG和Chg)和过氧化物增殖激活受体(PPARα和PPARγ)的表达而对海洋青鳉产生内分泌干扰效应,并且显示出性别特异性。MEHP对海洋青鳉的内分泌干扰效应弱于DEHP。

关键词(KeyWords)： 海洋青鳉(Oryzias melastigma);邻苯二甲酸二(2-乙基己)酯(DEHP);邻苯二甲酸单乙基己基酯(MEHP);雌激素受体(ER);卵黄蛋白原(VTG);卵膜蛋白原(Chg);过氧化物增殖激活受体(PPAR)

Abstract:

Keywords:

基金项目(Foundation): 国家自然科学基金(21277137,21107107,21207127);; 福建省自然科学基金(2011J05117)

作者(Authors): 叶婷;康美;黄乾生;董四君;

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