铜暴露下赤子爱胜蚓(Eisenia foetida)活体基因的损伤研究Detection of DNA Damage in Earthworm(Eisenia foetida)in Vivo Exposure to Copper Ion
卜元卿;骆永明;滕应;李振高;
摘要(Abstract)：
通过碱性单细胞凝胶电泳法研究了Cu2+暴露剂量对赤子爱胜蚓(Eiseniafoetida)活体基因损伤的动态影响.结果显示:不添加Cu2+的对照组和添加Cu2+的处理组蚯蚓体腔细胞尾部DNA含量和尾长均呈非正态分布(p<0.05);在暴露72h时,125mg·L-1Cu2+处理组尾部DNA含量值最大,为41.44%,100mg·L-1Cu2+处理组尾长值最大,为33.79μm;随着Cu2+暴露剂量的增加,尾部DNA含量和尾长损伤频率增加;对照组和处理组的尾部DNA含量和尾长之间均存在显著性差异(p<0.05),Spearman非参数相关分析表明,尾部DNA百分含量和尾长之间呈显著相关(p<0.01,n=21),Cu2+暴露浓度与尾部DNA百分含量、尾长具有良好的剂量-构效关系(p<0.01).在125mg·L-1Cu2+浓度下暴露72h时蚯蚓的基因损伤程度达到最大,损伤程度为3级.可见,蚯蚓DNA生物标志物是重金属污染基因毒理诊断的重要指标,碱性SCGE试验是检测Cu2+暴露对赤子爱胜蚓活体基因损伤的有效手段.
关键词(KeyWords)： 碱性单细胞凝胶电泳;Cu2+;蚯蚓;基因损伤;生物标志物
基金项目(Foundation): 国家自然科学基金重点项目(No.40432005);; 国家重点基础研究发展规划项目(No.2002CB410810/9)
作者(Authors): 卜元卿;骆永明;滕应;李振高;
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